職場內 構成員의 不適應 現象에 對한 硏究 : 따돌림을 중심으로

申東業,朴高勳 木浦大學校 敎育硏究所 2000 교육연구 Vol.13 No.-

For the investigation on the alienation in a job site, the questionnaire of this paper was made with reference to the model of German Prof. Heinz Leyman's 45 mobbing actions. The subjects of this questionnaire are 187 staff members of 5 companies located in Kangju and Jeonnam Province. According to the feature of an analyzed item, x² namely t-value is produced in order to inspect meaningful differences with the three units( .001, .01, .05 ). The results of what this paper is analyzed into are as follows: First, the duty valuation of the office senior is comparatively fairly accomplished, and all the behaviors of the senior becomes good example by his junior members. Secondly, the daily facing questions of the staff members are economy, health, human relationships, the opposite sex, the education of their children, etc. Thirdly, Alienation takes place in the office, but doesn't still become serious social affairs. Fourthly, most people who are ostracized in the office are egoistic, selfish, and capable of doing anything to gain their promotion. Finally, the ways of ostracizing are mostly personal isolations: communication isolation, information interception, and exclusion from meetings.

BioSMACK: a Linux Live CD for genome-wide association analyses

( Chang Bum Hong ),( Young Jin Kim ),( Sang Hoon Moon ),( Young Ah Shin ),( Min Jin Go ),( Dong Joon Kim ),( Jong Young Lee ),( Yoon Shin Cho ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.1

Recent advances in high-throughput genotyping technologies have enabled us to conduct a genome-wide association study (GWAS) on a large cohort. However, analyzing millions of single nucleotide polymorphisms (SNPs) is still a difficult task for researchers conducting a GWAS. Several difficulties such as compatibilities and dependencies are often encountered by researchers using analytical tools, during the installation of software. This is a huge obstacle to any research institute without computing facilities and specialists. Therefore, a proper research environment is an urgent need for researchers working on GWAS. We developed BioSMACK to provide a research environment for GWAS that requires no configuration and is easy to use. BioSMACK is based on the Ubuntu Live CD that offers a complete Linux-based operating system environment without installation. Moreover, we provide users with a GWAS manual consisting of a series of guidelines for GWAS and useful examples. BioSMACK is freely available at http://ksnp.cdc. go.kr/biosmack. [BMB reports 2012; 45(1): 44-46]

Two-dimensional attention-based multi-input LSTM for time series prediction

Kim, Eun Been,Park, Jung Hoon,Lee, Yung-Seop,Lim, Changwon The Korean Statistical Society 2021 Communications for statistical applications and me Vol.28 No.1

Time series prediction is an area of great interest to many people. Algorithms for time series prediction are widely used in many fields such as stock price, temperature, energy and weather forecast; in addtion, classical models as well as recurrent neural networks (RNNs) have been actively developed. After introducing the attention mechanism to neural network models, many new models with improved performance have been developed; in addition, models using attention twice have also recently been proposed, resulting in further performance improvements. In this paper, we consider time series prediction by introducing attention twice to an RNN model. The proposed model is a method that introduces H-attention and T-attention for output value and time step information to select useful information. We conduct experiments on stock price, temperature and energy data and confirm that the proposed model outperforms existing models.

기포유동층 고분자 중합 반응기에서의 슬러그 특성

고은솔 ( Eun Sol Go ),강서영 ( Seo Yeong Kang ),서수빈 ( Su Been Seo ),김형우 ( Hyung Woo Kim ),이시훈 ( See Hoon Lee ) 한국화학공학회 2020 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.58 No.4

고체 입자들이 유체처럼 움직이는 유동층 공정은 에너지 전환 공정뿐만 아니라 범용 고분자 수지의 생산 공정에도 이용되고 있다. 범용 고분자 수지 중의 하나인 LLDPE(Linear low density polyethylene)도 기포 유동층 공정을 통해 전세계에서 생산되고 있다. 입자 크기에 비해 밀도가 낮은 LLDPE 입자들은 고분자 중합 반응을 위해 공급되는 수소에 의해서 유동화된다. 그러나 LLDPE 생산 공정은 기포유동층 공정임에도 불구하고 발생한 슬러그로 인하여 반응에 영향을 끼쳐 공정의 효율 저하를 불러올 수 있다. 이에 본 연구에서는 상용 고분자 반응기를 모사한 pilot 규모의 고분자 합성 반응기(0.38 m l.D., 4.4 m High)와 동일한 시뮬레이션 모델을 구축하여 LLDPE 입자의 유동화 상태를 고찰하였다. 특히 기체 유속(0.45-1.2 m/s), 고체 입자 밀도(900-1900 kg/㎥), 입자 구형도(0.5-1.0), 입자 크기(120-1230 μm)의 변화에 따른 슬러그 특성을 세밀하게 고찰하기 위하여 전산입자유체해석(Computational particle-fluid dynamics, CPFD)을 이용하였다. CPFD를 통해서 일부 실험자들만 고찰할 수 있었던 flat slug의 발생을 시각적으로 구현하였으며 밀도, 구형도, 크기 등의 고체의 물리적 특성을 변화시킴에 따라 슬러그 발생을 저감시킬 수 있음을 확인하였다. Fluidization processes in which solid particles vividly move like gas or liquid have been widely used in various industrial sectors, such as thermochemical energy conversion and polymerization processes for general purpose polymer resins. One of the general purpose polymer resins, LLDPE(Linear low-density polyethylene) resins have been produced in bubbling fluidized bed processes in the world. In a bubbling fluidization polymerization reactors, LLDPE particles with relatively larger particle size and low density are fluidized by hydrogen gas for polymerization reaction. Though LLDPE polymerization reactors are one of bubbling fluidization processes, slugs that have negative impact for reaction exist or occur in these processes. Therefore, the fluidization state of LLDPE particles was investigated in a simulation model similar to a pilot-scale polymerization reactor (0.38 m l.D., 4.4 m High). In particular, the effect of gas velocity (0.45-1.2 m/s), solid density (900-199 kg/㎥), solid sphericity (0.5-1.0), and average particle size (120-1230 μm), on bed height and fluidization state were measured by using a CPFD(Computational particle-fluid dynamics) method. With CPFD analysis, the occurrence of a flat slug was visualized. Also, the change in particle properties, such as particle density, sphericity, and size, could reduce the occurrence of slug and bed expansion.

Transcriptome analysis to identify long non coding RNA (lncRNA) and characterize their functional role in back fat tissue of pig

Kumar, Himansu,Srikanth, Krishnamoorthy,Park, Woncheol,Lee, Seung-Hoon,Choi, Bong-Hwan,Kim, Hana,Kim, Yong-Min,Cho, Eun-Seok,Kim, Jin Hyoung,Lee, Jang Hee,Jung, Ji Yeon,Go, Gwang-woong,Lee, Kyung-Tai Elsevier 2019 Gene Vol.703 No.-

<P><B>Abstract</B></P> <P>Long non coding RNAs (lncRNA) have been previously found to be involved in important cellular activities like epigenetics, implantation, cell growth etc. in pigs. However, comprehensive analysis of lncRNA in back fat tissues at different developmental stages in pigs is still lacking. In this study we conducted transcriptome analysis in the back fat tissue of a F1 crossbred Korean Native Pig (KNP) × Yorkshire Pig to identify lncRNA. We investigated their role in 16 pigs at two different growth stages; stage 1 (10 weeks, n = 8) and stage 2 (26 weeks, n = 8). After quality assessment of sequencing reads, we got a total of 1,641,165 assembled transcripts out of eight paired end read from each stage. Among them, 6808 lncRNA transcripts were identified by filtering on the basis of multiple parameters like read length ≥ 200 nucleotides, exon numbers ≥2, FPKM ≥0.5, coding potential score < 0 etc. PFAM and RFAM were used to filter out all possible protein coding genes and housekeeping RNAs respectively. A total of 103 lncRNAs and 1057 mRNAs were found to be differentially expressed (DE) between the two stages (|log2FC| > 2, q < 0.05). We also identified 306 genes located around 100 kb upstream and 234 genes downstream around these DE lncRNA transcripts. The expression of top eleven DE lncRNAs (COL4A6, LY7S, MYH2, OXCT1, SMPDL3A, TMEM182, TTC36, RFOOOO4, RFOOO15, RFOOO45, CADM2) had been validating by qRT-PCR. Pathway and GO terms analysis showed that, positive regulation of biosynthetic process, Wnt signaling pathway, cellular protein modification process, and positive regulation of nitrogen compound were differentially enriched. Our results suggested that, KEGG pathways such as protein digestion and absorption, Arrhythmogenic right ventricular cardiomyopathy (ARVC) to be significantly enriched in both DE lncRNAs as well as DE mRNAs and involved in back fat tissues development. It also suggests that, identified lncRNAs are involved in regulation of important adipose tissues development pathways.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Analysis of lncRNAs in back fat tissues at different developmental stages in the pigs </LI> <LI> A total of 103 lncRNAs and 1057 mRNAs were found to be differentially expressed. </LI> <LI> KEGG pathway and GO analysis for functional annotation of lncRNAs </LI> <LI> Top DE transcripts were validated by qRTPCR. </LI> <LI> Relation between back fat metabolism and lncRNAs were established. </LI> </UL> </P>

항암제 유발 신경독성을 관리하는 한의학 및 보완대체요법들과 임상시험 현황

박선주 ( Sun Ju Park ),고호연 ( Ho Yeon Go ),한유진 ( Yoo Jin Han ),고성규 ( Seong Gyu Ko ),김성훈 ( Sung Hoon Kim ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.4

Objectives: Cancer incidence is increasing in all countries and chemotherapy-induced peripheral neuropathy (CIPN) in patients undergoing chemotherapeutic agents have been a clinically serious problems. So far therapeutic options for CIPN patients are limited and no confirmed methods have yet been established for dealing with peripheral neuropathy. Therefore this review is to provide an evidence-based summary of oriental medicine and CAM (complementary and alternative medicine) neuroprotective and treatment therapies which have gone through clinical trials. Methods: An overview of the domestic and international papers of adult clinical trials relating management of only CIPN symptoms through 1990 to present were searched by electronic databases. Search key words were chemotherapy-induced neurotoxicity, chemotherapy-induced peripheral neuropathy, chemotherapy toxicity & herb, chemotherapy toxicity & acupuncture, chemotherapy toxicity & CAM. Only English and Korean written papers were reviewed. Total 25 papers were reviewed in this study, 18 papers were retrieved by electronic search. Results: Clinical studies of managing CIPN were rare, two acupuncture clinical studies and four herb medicinal studies were found. Rest of 19 papers were about other CAM clinical studies. Total 25 papers were analyzed, and all interventions were focused on their pain control efficacy. Other 24 trials of potential therapies except one proved to be effective for CIPN, however some described to be inadequate positive or sufficient negative. Conclusions: As most of the studies were pilot studies, interventions for the prevention and treatment of CIPN have to go through prospective confirmatory studies, such as larger scale randomized, double-blinded, placebo controlled clinical trials must be done for the safe and effective use of proposed therapies. Also standard measurement scales have to be developed for the better clinical study of CIPN.

태양열 하이브리드 공정을 위한 유동층 입자들의 마모 및 열전달 특성 연구

김형우(Hyung Woo Kim),이도연(Doyeon Lee),남형석(Hyungseok Nam),홍영완(Young Wan Hong),서수빈(Su Been Seo),고은솔(Eun Sol Go),강서영(Seo Yeong Kang),이시훈(See Hoon Lee) 한국청정기술학회 2020 청정기술 Vol.26 No.1

전기와 천연가스와 같이 안정적이며 신뢰할 수 있는 에너지를 현대 사회가 요구하기 때문에 재생에너지와 화석연료의 장점들을 모두 보유하고 있는 다양한 방식의 태양열 하이브리드 공정들이 세계 각국에서 개발되고 있다. 특히 고체 입자에 태양열을 저장하는 유동층 기반의 태양열 하이브리드 공정은 기존의 유동층 연소 및 가스화에 적용할 수 있을 것으로 기대받고 있다. 이에 본 연구에서는 ASTM D5757 반응기와 0.14 m의 직경과 2 m 높이의 유동층 반응기를 이용하여 태양열 하이브리드 공정의 유동층물질로서 검토되고 있는 실리콘 카바이드, 알루미나 입자들의 마모 및 열전달 특성을 고찰하였다. 특히 다양한 상업 유동층 반응기에서 유동층물질로 이용되는 모래와 비교하였다. 실리콘카바이드와 알루미나의 내마모성은 모래보다 우수하였으며 평균 열전달 계수도 125 ~ 152 W m<SUP>-2</SUP><SUP></SUP>K<SUP>-1</SUP> 범위를 가지는 것으로 고찰되었다. Various solar hybrid energy conversion processes, which have both the advantages of renewable energy sources and fossil energy sources, have been developed in the world because stable and predictable energy supplies, such as electricity and natural gas, are necessary for modern societies. In particular, a solar hybrid energy conversion process based on a dual fluidized bed process concept has been expected as the promising solution for sustainable energy supply via thermochemical conversions, such as pyrolysis, combustion, gasification, and so on, because solar thermal energy could be captured and stored in fluidized bed materials. Therefore, the attrition and heat transfer characteristics of silicon carbide and alumina particles used for fluidized bed materials for the solar hybrid energy conversion process were studied in an ASTM D5757 reactor and a bubbling fluidized bed reactor with 0.14m diameter and 2m height. These characteristics of novel fluidized bed materials were compared with those of sand particles which have widely been used as a fluidized bed material in various commercial fluidized bed reactors. The attrition resistances of silicon carbide and alumina particles were higher than those of sand particles while the average values of heat transfer coefficient in the bubbling fluidized bed reactor were in the range of 125 ~ 152 W m<SUP>-2</SUP>K<SUP>-1</SUP>.

Piperlongumine decreases cognitive impairment and improves hippocampal function in aged mice

Go, Jun,Park, Tae-Shin,Han, Geun-Hee,Park, Hye-Yeon,Ryu, Young-Kyoung,Kim, Yong-Hoon,Hwang, Jung Hwan,Choi, Dong-Hee,Noh, Jung-Ran,Hwang, Dae Youn,Kim, Sanghee,Oh, Won Keun,Lee, Chul-Ho,Kim, Kyoung-Sh D.A. Spandidos 2018 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.42 No.4

<P>Piperlongumine (PL), a biologically active compound from the <I>Piper</I> species, has been shown to exert various pharmacological effects in a number of conditions, including tumours, diabetes, pain, psychiatric disorders and neurodegenerative disease. In this study, we evaluated the therapeutic effects of PL on hippocampal function and cognition decline in aged mice. PL (50 mg/kg/day) was intragastrically administrated to 23-month-old female C57BL/6J mice for 8 weeks. Novel object recognition and nest building behaviour tests were used to assess cognitive and social functions. Additionally, immunohistochemistry and western blot analysis were performed to examine the effects of PL on the hippocampus. We found that the oral administration of PL significantly improved novel object recognition and nest building behaviour in aged mice. Although neither the percentage area occupied by astrocytes and microglia nor the level of 4-hydroxynonenal protein, a specific marker of lipid peroxidation, were altered by PL treatment, the phosphorylation levels of <I>N</I>-methyl-D-aspartate receptor subtype 2B (NR2B), calmodulin-dependent protein kinase II alpha (CaMKIIα) and extracellular signal-regulated kinase 1/2 (ERK1/2) were markedly increased in the hippocampus of aged mice following the administration of PL. We also found that PL treatment resulted in a CA3-specific increase in the phosphorylation level of cyclic AMP response element binding protein, which is recognized as a potent marker of neuronal plasticity, learning and memory. Moreover, the number of doublecortin-positive cells, a specific marker of neurogenesis, was significantly increased following PL treatment in the dentate gyrus of the hippocampus. On the whole, these data demonstrate that PL treatment may be a potential novel approach in the treatment of age-related cognitive impairment and hippocampal changes.</P>

Small heterodimer partner deficiency exacerbates binge drinking-induced liver injury via modulation of natural killer T cell and neutrophil infiltration

Go, Min-Jeong,Noh, Jung-Ran,Hwang, Jung Hwan,Kim, Kyoung-Shim,Choi, Dong-Hee,Lee, Jong-Soo,Kim, Yong-Hoon,Lee, Chul-Ho D.A. Spandidos 2018 MOLECULAR MEDICINE REPORTS Vol.17 No.4

<P>Binge drinking among alcohol consumers is a common occurrence, and may result in the development of numerous diseases, including liver disorders. It has previously been reported that natural killer T (NKT) cells induce alcohol-associated liver injury by promoting neutrophil infiltration. In the present study, the role of the orphan nuclear receptor small heterodimer partner (SHP), which is encoded by the NR0B2 gene, in acute binge drinking-induced liver injury was investigated. SHP-knockout (KO) and wild-type (WT) control mice were intragastrically administered single doses of alcohol. The plasma concentrations of alanine aminotransferase and aspartate aminotransferase in SHP-KO mice following alcohol treatment were significantly increased compared with WT mice. However, results of oil red O staining and 2′,7′-dichlorodihydrofluorescein diacetate staining indicated that levels of acute binge drinking-associated hepatic lipid accumulation and oxidative stress were not significantly different between WT and SHP-KO alcohol-treated mice. Notably, tumor necrosis factor-α mRNA expression in the liver of SHP-KO mice was significantly increased following alcohol administration, compared with WT mice. Furthermore, the mRNA expression levels of C-C motif chemokine ligand 2, C-X-C motif chemokine ligand 2 and interleukin-4, which are all potent chemoattractants of NKT cells, as well as neutrophil expression levels, were significantly increased in the livers of SHP-KO mice compared with WT mice following alcohol administration, as determined by reverse transcription-quantitative polymerase chain reaction and flow cytometry. Enhanced infiltration of NKT cells, determined by flow cytometry, was also demonstrated in the livers of SHP-KO mice following alcohol administration, compared with WT mice. The results of the present study indicate that SHP may be involved in liver-associated protective mechanisms, with regards to the attenuation of damage caused by acute binge drinking, via regulation of NKT cell and neutrophil migration to the liver. The modulation of SHP may be a novel therapeutic strategy for the treatment of acute binge drinking-induced liver injury.</P>

HPV-18 E7 Interacts with Elk-1 Leading to Elevation of the Transcriptional Activity of Elk-1 in Cervical Cancer

Go Sung-Ho,Rho Seung Bae,Yang Dong-Wha,Kim Boh-Ram,Lee Chang Hoon,Lee Seung-Hoon 한국응용약물학회 2022 Biomolecules & Therapeutics(구 응용약물학회지) Vol.30 No.6

The human papillomavirus (HPV)-18 E7 (E7) oncoprotein is a major transforming protein that is thought to be involved in the development of cervical cancer. It is well-known that E7 stimulates tumour development by inactivating pRb. However, this alone cannot explain the various characteristics acquired by HPV infection. Therefore, we examined other molecules that could help explain the acquired cancer properties during E7-induced cancer development. Using the yeast two-hybrid (Y2H) method, we found that the Elk-1 factor, which is crucial for cell proliferation, invasion, cell survival, anti-apoptotic activity, and cancer development, binds to the E7. By determining which part of E7 binds to which domain of Elk-1 using the Y2H method, it was found that CR2 and CR3 of the E7 and parts 1–206, including the ETS-DNA domain of Elk-1, interact with each other. As a result of their interaction, the transcriptional activity of Elk-1 was increased, thereby increasing the expression of target genes EGR-1, c-fos, and E2F. Additionally, the colony forming assay revealed that overexpression of Elk-1 and E7 promotes C33A cell proliferation. We expect that the discovery of a novel E7 function as an Elk-1 activator could help explain whether the E7 has novel oncogenic activities in addition to p53 inactivation. We also expect that it will offer new methods for developing improved strategies for cervical cancer treatment.