The Bacterial Surface Expression of SARS Viral Epitope using Salmonella typhi Cytolysin A

Piao, Hong-Hua,Seong, Ji-Hyoun,Song, Man-Ki,Kim, Youn-Uck,Shin, Dong-Jun,Choy, Hyon-E,Hong, Yeong-Jin The Korean Society for Microbiology 2009 Journal of Bacteriology and Virology Vol.39 No.2

The cytolysin A (ClyA) is a 34 kDa pore-forming cytotoxic protein and expressed by some enteric bacteria including Salmonella typhi. This toxin is transported on the bacterial surface and secreted without posttranslational modification. Using the surface display of ClyA, the expression vectors for 193-aa immunogenic antigen of spike protein (termed S1E) from severe acute respiratory syndrome coronavirus (SARS-CoV) were constructed. The vectors carried a gene encoding S. typhi ClyA conjugated to S1E at the C terminus (termed ClyA-S1E) and asd gene in pGEM-T and pBR322, named pGApLCS1E and pBApLCS1E, respectively. An asd-mutated E. coli transformed with these vectors could grow without diaminopimelic acid (DAP), indicating that they were stably maintained in such mutants. ClyA-S1E recombinant proteins from these vectors were expressed on the surface of the attenuated S. typhimurium deficient of global virulence gene regulator, ppGpp. However, they did not show the hemolytic activity on the blood agar plate and cytotoxicity against HeLa cells. To examine whether bacteria expressing ClyA-S1E induced the immune response against S1E, S. typhimurium deficient of ppGpp and Asd was transformed with these vectors and orally immunized in mice. In the western blotting against GST-conjugated S1E using the immunized mouse sera, it was shown that the significant band was detected in the mouse serum by the bacteria transformed with pGApLCS1E but not with pBApLCS1E. It indicates that the immune response producing antibody was dependent on the expression level of ClyA-S1E. Therefore, ClyA delivery system can be used for SARS vaccine development.

화력발전소의 대기오염물질 배출계수 산정 연구

김대곤,엄윤성,홍지형,이석조,석광설,이대균,이은정,방선애 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.3

The main purpose of this study was to characterize the air pollutants emission factors in electric power plant (EPP) using fossil fuels. The electric power plant is a major air pollution source, thus knowing the emission characteristics of electric power plant is very important to develop a control strategy. The major air pollutants of concern from EPP stacks are particulate matter (PM), sulfur oxides (SOx), nitrogen oxides (NOx), carbon monoxide (CO) and heavy metals. Throughout the study. the following results arc estimated: - PM : 8.671E-05∼8.724E+01 PM emission (kg) per fuel burned (ton) - SOx: 4.749E-04∼7.877E+01 SOx emission (kg) per fuel burned (ton) - NOx : 1.578E-02∼9.857E+00 NOx emission (kg) per fuel burned (ton) - CO : 3.800E-04∼1.291E+00 CO emission (kg) per fuel burned (ton) - Hg : 1.220E+01∼3.108E+02 Hg emission (mg) per fuel burned (ton) From the statistical analysis by Wilcoxon signed ranks test between the emission factors of ours and U.S. EPA's. we can yielded that: p > 0.05.

철강산업 용융로의 대기오염물질 배출계수 산정 연구

석광설,방선애,홍지형,이석조,김대곤,이대균,허정숙,이은정 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.4

The purpose of this study is 10 estimate of emission factors of the air pollutants for the melting furnaces for the iron and steel industry. The result of this study is able to obtain the emission factor of particulate matters (PM), sulfur dioxide, nitrogen oxides for melting furnace. The emission factors of each pollutants were as follows: - the emission factor varied between 6.13E-03 ∼ 6.12E-01kg/ton for PM - 1.59E-01 ∼ 2.45E+00kg/ton for S0₂ - 6.82E-02 ∼ 6.88E-01kg/ton for NOx, respectively. Analysis of the differences in the emission factors of ours and U.S. EPA's yielded the following results for the Wilcoxon method: p>0.05. The statistical analysis showed no differences in the our emission factors and U.S. EPA's

腦炎發生豫測에 對한 病毒學的 昆蟲學的 調査硏究(1972)

申鶴均,李漢一,朴基德,洪漢基,鄭義範,金薄榮,李溫永,金慶浩 國立保健硏究院 1974 국립보건연구원보 Vol.11 No.-

Effects of estrogen and estrogenic compounds, 4-tert-octylphenol, and bisphenol A on the uterine contraction and contraction-associated proteins in rats

An, B.S.,Ahn, H.J.,Kang, H.S.,Jung, E.M.,Yang, H.,Hong, E.J.,Jeung, E.B. North-Holland 2013 Molecular and cellular endocrinology Vol.375 No.1

We examined the effects of estradiol (E2), 4-tert-octylphenol (OP), and bisphenol A (BPA) on uterine contractions in immature rats. The expression and localization of contraction-associated proteins (CAPs), and contractility of rat uterus with a collagen gel contraction assay were analyzed. E2, OP, and BPA all increased oxytocin (OT)-related pathway, while the prostaglandin-related signaling was reduced. Interestingly, E2 and estrogenic compounds showed distinct effects on the contractile activity of uterine cells. E2 enhanced the contractility, while OP and BPA significantly decreased it. Immunohistochemical analysis of CAPs showed distinct regulation of prostaglandin F receptor localization by E2 and estrogenic compounds, which may explain the different contractile activities of those reagents. In summary, we demonstrate that E2, OP, and BPA regulate CAP expression in a similar manner in the immature rat uterus, however, the effects on contractile activity were modulated differently. These findings suggest that OP and BPA interfere with uterine contractility.

Parasiticidal activity of a novel synthetic peptide from the core α-helical region of NK-lysin

Lee, S.H.,Lillehoj, H.S.,Tuo, W.,Murphy, C.A.,Hong, Y.H.,Lillehoj, E.P. Elsevier Scientific Pub. Co 2013 Veterinary parasitology Vol.197 No.1

NK-lysin is an anti-microbial peptide that plays a critical role in innate immunity against infectious pathogens through its selective membrane disruptive property. We previously expressed and purified a full-length chicken NK-lysin (cNKL) recombinant protein, and demonstrated its in vitro anti-parasitic activity against the apicomplexan protozoan, Eimeria, the etiologic agent of avian coccidiosis. This study evaluated the in vitro and in vivo anti-parasitic properties of a synthetic peptide (cNK-2) incorporating a predicted membrane-permeating, amphipathic α-helix of the full-length cNKL protein. The cNK-2 peptide exhibited dose- and time-dependent in vitro cytotoxic activity against E. acervulina and E. tenella sporozoites. The cytotoxic activity of 1.5μM of cNK-2 peptide against E. acervulina following 6h incubation was equal to that of 2.5μM of melittin, the principal active component of apitoxin (bee venom) that also exhibits anti-microbial activity. Even greater activity was detected against E. tenella, where 0.3μM of cNK-2 peptide was equivalent to 2.5μM of melittin. Against Neospora caninum tacyzoites, however, the cytotoxic activity of cNK-2 peptide was inferior to that of melittin. Transmission electron microscopy of peptide-treated E. tenella sporozoites revealed disruption of the outer plasma membrane and loss of intracellular contents. In vivo administration of 1.5μM of cNK-2 peptide increased protection against experimental E. acervulina infection, as measured by greater body weight gain and reduced fecal oocyst shedding, compared with saline controls. These results suggest that the cNK-2 synthetic peptide is a novel anti-infective peptide that can be used for protection against avian coccidiosis during commercial poultry production.

SAHA, an HDAC inhibitor, overcomes erlotinib resistance in human pancreatic cancer cells by modulating E-cadherin

Park, S. J.,Kim, S. M.,Moon, J. H.,Kim, J. H.,Shin, J. S.,Hong, S. W.,Shin, Y. J.,Lee, D. H.,Lee, E. Y.,Hwang, I. Y. Springer Science + Business Media 2016 Tumour biology Vol.37 No.4

<P>Pancreatic cancer is one of the most lethal cancers and remains a major unsolved health problem. Less than 20 % of patients are surgical candidates, and the median survival for non-resected patients is approximately 3 to 4 months. Despite the existence of many conventional cancer therapies, few targeted therapies have been developed for pancreatic cancer. Combination therapy using erlotinib and gemcitabine is an approved standard chemotherapy for advanced pancreatic cancer, but it has marginal therapeutic benefit. To try to improve the therapeutic outlook, we studied the efficacy of another combination treatment and the relevance to E-cadherin in human pancreatic cancer cells. We treated two human pancreatic cancer cell lines with the histone deacetylase inhibitor (HDACi) SAHA. Interestingly, in these Panc-1 and Capan1 cells, we observed that the expression levels of E-cadherin and phosphorylated EGFR were gradually upregulated after treatment with SAHA. Furthermore, these cells underwent induced cell death after exposure to the combination treatment of SAHA and erlotinib. In Panc-1 cells, overexpression of E-cadherin activated the phosphorylation of EGFR and increased the cell sensitivity to erlotinib. In Capan1 cells, knocking down E-cadherin decreased the expression of phosphorylated EGFR, and these cells did not respond to erlotinib. Therefore, we demonstrated the efficacy of the combined treatment with SAHA and erlotinib in human pancreatic cancer cells, and we determined that the increased efficacy was due, at least in part, to the effects of SAHA on the expression of E-cadherin. Our studies suggest that E-cadherin may be a potent biomarker for pancreatic cancer.</P>

Small-scale effects of thermal inflation on halo abundance at high- z , galaxy substructure abundance, and 21-cm power spectrum

Hong, Sungwook E.,Zoe, Heeseung,Ahn, Kyungjin American Physical Society 2017 Physical Review D Vol.96 No.10

<P>We study the impact of thermal inflation on the formation of cosmological structures and present astrophysical observables which can be used to constrain and possibly probe the thermal inflation scenario. These are dark matter halo abundance at high redshifts, satellite galaxy abundance in the Milky Way, and fluctuation in the 21-cm radiation background before the epoch of reionization. The thermal inflation scenario leaves a characteristic signature on the matter power spectrum by boosting the amplitude at a specific wave number determined by the number of e-foldings during thermal inflation (Nbc), and strongly suppressing the amplitude for modes at smaller scales. For a reasonable range of parameter space, one of the consequences is the suppression of minihalo formation at high redshifts and that of satellite galaxies in the Milky Way. While this effect is substantial, it is degenerate with other cosmological or astrophysical effects. The power spectrum of the 21-cm background probes this impact more directly, and its observation may be the best way to constrain the thermal inflation scenario due to the characteristic signature in the power spectrum. The Square Kilometre Array (SKA) in phase 1 (SKA1) has sensitivity large enough to achieve this goal for models with N-bc greater than or similar to 26 if a 10000-hr observation is performed. The final phase SKA, with anticipated sensitivity about an order of magnitude higher, seems more promising and will cover a wider parameter space.</P>

EPSTEIN-BARR VIRUS ANTIBODY LEVELS IN KOREANS

Hong, Chang Yee,Lee, Ho Sung,Henle, Werner,Henle, Gertrude E. 대한의학협회 1977 대한의학협회지 Vol.20 No.5

Dipeptidyl peptidase 4 inhibitor attenuates obesity-induced myocardial fibrosis by inhibiting transforming growth factor-βl and Smad2/3 pathways in high-fat diet-induced obesity rat model

Hong, S.K.,Choo, E.H.,Ihm, S.H.,Chang, K.,Seung, K.B. W.B.Saunders [etc.] 2017 clinical and experimental Vol.76 No.-

<P>Obesity-induced myocardial fibrosis may lead to diastolic dysfunction and ultimately heart failure. Activation of the transforming growth factor (TGF)-beta 1 and its downstream Smad2/3 pathways may play a pivotal role in the pathogenesis of obesity-induced myocardial fibrosis, and the antidiabetic dipeptidyl peptidase 4 inhibitors (DPP4i) might affect these pathways. We investigated whether DPP4i reduces myocardial fibrosis by inhibiting the TGF-beta 1 and Smad2/3 pathways in the myocardium of a diet-induced obesity (DIO) rat model. Eight-week-old male spontaneously hypertensive rats (SHRs) were fed either a normal fat diet (chow) or a high-fat diet (HFD) and then the HFD-fed SHRs were randomized to either the DPP4i (MK-0626) or control (distilled water) groups for 12 weeks. At 20 weeks old, all the rats underwent hemodynamic and metabolic studies and Doppler echocardiography. Compared with the normal fat diet (chow)-fed SHRs, the HFD-fed SHRs developed a more intense degree of hyperglycemia and dyslipidemia and showed a constellation of left ventricular (LV) diastolic dysfunction, and exacerbated myocardial fibrosis, as well as activation of the TGF-beta 1 and Smad2/3 pathways. DPP4i significantly improved the metabolic and hemodynamic parameters. The echocardiogram showed that DPP4i improved the LV diastolic dysfunction (early to late ventricular filling velocity [E/A] ratio, 1.49 +/- 0.21 vs. 1.77 +/- 0.09, p < 0.05). Furthermore, DPP4i significantly reduced myocardial fibrosis and collagen production by the myocardium and suppressed TGF-beta 1 and phosphorylation of Smad2/3 in the heart. In addition, DPP4i decreased TGF-beta 1-induced collagen production and TGF-beta 1-mediated phosphorylation and nuclear translocation of Smad2/3 in rat cardiac fibroblasts. In conclusion, DPP4 inhibition attenuated myocardial fibrosis and improved LV diastolic dysfunction in a DIO rat model by modulating the TGF-beta 1 and Smad2/3 pathways. (C) 2017 Elsevier Inc. All rights reserved.</P>