LC-ESI-MS/MS를 이용한 생체시료 중 브롬화피나베리움의 고감도 분석 및 이를 이용한 생체이용률 평가 : Applicability to Oral Bioavailability Determination

박석,이예리,김호현,이희주,김윤균,염정록,한상범 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.6

A sensitive method for quantification of pinaverium bromide in human plasma was established using liquid chromatography-electrospray ionization tandem mass spectrometrv(LC-ESI-MS/MS). Glimepiride was used as internal standard. Pinaverium bromide and internal standard in plasma sample were extracted using tert-but}lmethvlether(TBME). A centrifuged upper laver was then evaporated and reconstituted with mobile phase of acetonitrile-5 m1VI ammonium formate (8020. pH 3.0). The reconstituted samples were injected into a C_(18) reversed-phase column. Using MS/MS with multiple reaction monitoring (MRM) mode. pinaverium and glimepirde were detected without severe interference from human plasma matrix. Pinaverium produced a protonated precursor ion ([M+H]^(+)) at m/z 510.3 and a corresponding product ion at m/z 228.9. Internal standard produced a protonated precursor ion ([M+H] ^(+)) at m/z 491.5 and a corresponding product ion at m/z 352.0. Detection of pinaverium bromide in human plasma was accurate and precise. with limit of quantitation at 0.5 ng/ml. The method has been successfully applied to bioavailability study of pinaverium bromide tablet in Korean healthy male volunteers. Pharmacokinetic parameters such as AUCr. C_(max) T_(max). K_(el) and t_(l/2) were calculated.

Study on the Single Dose Toxicity of ShinEumHur Pharmacopuncture Injected into the Muscles of Rats

Jeong, Hohyun,Cha, Eunhye,Lee, Jongcheol,Lee, Seongjin,Park, Manyong,Kim, Dongwoung,Song, Bongkeun,Lee, Jongdeok,Kim, Sungchul KOREAN PHARMACOPUNCTURE INSTITUTE 2016 Journal of pharmacopuncture Vol.19 No.4

Objectives: This study was carried out to analyze the single dose toxicity of ShinEumHur (SEH) pharmacopuncture injected into the muscles of Sprague-Dawley rats. Methods: The SEH pharmacopuncture was made in a clean room at the Korean Pharmacopuncture Institute (K-GMP). After the mixing process with sterile distilled water had been completed, the pH was controlled to between 7.0 and 7.5. All experiments were conducted at Biotoxtech, an institution authorized to perform non-clinical studies under the Good Laboratory Practice (GLP) regulations. Sprague-Dawley rats were chosen for the pilot study. Doses of SEH pharmacopuncture, 0.25, 0.5 and 1.0 mL, were administered to the experimental groups, and a dose of normal saline solution, 1.0 mL, was administered to the control group. We examined the survival rate, weights, clinical signs, mean hematology parameters, mean clinical chemistry, necropsy and histopathological findings. This study was conducted under the approval of the Institutional Animal Ethics Committee. Results: No deaths or abnormalities occurred in any of the four groups. No significant changes in weight, hematological parameters or clinical chemistry between the control group and the experimental groups were observed. To check for abnormalities in organs and tissues, we used microscopy to examine representative histological sections of each specified organ; the results showed no significant differences in any of the organs or tissues. Conclusion: The above findings suggest that treatment with SEH pharmacopuncture is relatively safe. Further studies on this subject are needed to yield more concrete evidence.

Comparison the Mapping Accuracy of Construction Sites Using UAVs with Low-Cost Cameras

Jeong, Hohyun,Ahn, Hoyong,Shin, Dongyoon,Choi, Chuluong The Korean Society of Remote Sensing 2019 大韓遠隔探査學會誌 Vol.35 No.1

The advent of a fourth industrial revolution, built on advances in digital technology, has coincided with studies using various unmanned aerial vehicles (UAVs) being performed worldwide. However, the accuracy of different sensors and their suitability for particular research studies are factors that need to be carefully evaluated. In this study, we evaluated UAV photogrammetry using smart technology. To assess the performance of digital photogrammetry, the accuracy of common procedures for generating orthomosaic images and digital surface models (DSMs) using terrestrial laser scanning (TLS) techniques was measured. Two different type of non-surveying camera(Smartphone camera, fisheye camera) were attached to UAV platform. For fisheye camera, lens distortion was corrected by considering characteristics of lens. Accuracy of orthoimage and DSM generated were comparatively analyzed using aerial and TLS data. Accuracy comparison analysis proceeded as follows. First, we used Ortho mosaic image to compare the check point with a certain area. In addition, vertical errors of camera DSM were compared and analyzed based on TLS. In this study, we propose and evaluate the feasibility of UAV photogrammetry which can acquire 3 - D spatial information at low cost in a construction site.

Single Dose Toxicity of Chukyu (spine-healing) Pharmacopuncture Injection in the Muscle of Rats

Jeong, Hohyun,Cho, Seung-Hun,Lee, Eun-Yong,Lee, Seung-Deok,Ahn, Seong-Hun,Kim, Sungchul KOREAN PHARMACOPUNCTURE INSTITUTE 2014 Journal of pharmacopuncture Vol.17 No.1

Objectives: This study was performed to analyze the single dose toxicity of Chukyu (spine-healing) pharmacopuncture. Methods: All experiments were conducted at the Biotoxtech, an institution authorized to perform non-clinical studies under the regulations of Good Laboratory Practice (GLP) regulations. Sprague-Dawley rats were chosen for the pilot study. Doses of Chukyu (spine-healing) pharmacopuncture, 0.1, 0.5 and 1.0 mL, were administered to the experimental groups, and a dose of normal saline solution, 1.0 mL, was administered to the control group. This study was conducted under the approval of the Institutional Animal Ethic Committee. Results: No deaths or abnormalities occurred in any of the four groups. No significant changes in weight, hematological parameters or clinical chemistry between the control group and the experimental groups were observed. To check for abnormalities in organs and tissues, we used microscopy to examine representative histological sections of each specified organ; the results showed no significant differences in any of the organs or tissues except in one case, where interstitial infiltrating macrophages were found in one female rat in the 0.5-mL/animal experimental group. Conclusion: The above findings suggest that treatment with Chukyu (spine-healing) pharmacopuncture is relatively safe. Further studies on this subject are needed to yield more concrete evidence.

Feasibility of Using an Automatic Lens Distortion Correction (ALDC) Camera in a Photogrammetric UAV System

Jeong, Hohyun,Ahn, Hoyong,Park, Jinwoo,Kim, Hyungwoo,Kim, Sangseok,Lee, Yangwon,Choi, Chuluong 한국측량학회 2015 한국측량학회지 Vol.33 No.6

A Study on the Oral Toxicity of Mecasin in Rats

Jeong, Hohyun,Lee, Jongchul,Cha, Eunhye,Park, Manyong,Son, Ilhong,Song, Bongkeun,Kim, Sungchul KOREAN PHARMACOPUNCTURE INSTITUTE 2014 Journal of pharmacopuncture Vol.17 No.4

Objectives: In this study, we investigated the oral toxicity of Gami-Jakyak Gamcho buja Decoction (Mecasin) to develop safe treatments. Methods: All experiments were conducted at the Medvill, an institution authorized to perform non-clinical studies, under the Good Laboratory Practice (GLP) regulations. In order to investigate the oral toxicity of Mecasin, we administered Mecasin orally to rats. Sprague-Dawley rats were divided into four groups of five male and five female animals per group: group 1 being the control group and groups 2, 3, and 4 being the experimental groups. Doses of Mecasin, 500 mg/kg, 1,000 mg/kg and 2,000 mg/kg, were administered to the experimental groups, and a dose of normal saline solution, 10 mL/kg, was administered to the control group. We examined the survival rate, weight, clinical signs, and gross findings. This study was conducted under the approval of the Institutional Animal Ethics Committee. Results: No deaths or abnormalities occurred in any of the four groups. Although slight decreases in the weights of some female rats were noted on the third day, no significant changes in weights or gross findings between the control group and the experimental groups were observed. To check for abnormalities in organs, we used microscopy to examine representative histological sections of each specified organ; the results showed no significant differences in any of the organs. Conclusion: The results showed that administration of 500 - 2,000 mg/kg of Mecasin did not cause any changes in weight or in the results of necropsy examinations. It also did not result in any mortalities. The above findings suggest that treatment with Mecasin is relatively safe. Further studies on this subject are needed to yield more concrete evidence.

Comparison the Mapping Accuracy of Construction Sites Using UAVs with Low-Cost Cameras

Hohyun Jeong,Hoyong Ahn,Dongyoon Shin,ChulUong Choi 대한원격탐사학회 2019 大韓遠隔探査學會誌 Vol.35 No.1

The advent of a fourth industrial revolution, built on advances in digital technology, has coincided with studies using various unmanned aerial vehicles (UAVs) being performed worldwide. However, the accuracy of different sensors and their suitability for particular research studies are factors that need to be carefully evaluated. In this study, we evaluated UAV photogrammetry using smart technology. To assess the performance of digital photogrammetry, the accuracy of common procedures for generating orthomosaic images and digital surface models (DSMs) using terrestrial laser scanning (TLS) techniques was measured. Two different type of non-surveying camera(Smartphone camera, fisheye camera) were attached to UAV platform. For fisheye camera, lens distortion was corrected by considering characteristics of lens. Accuracy of orthoimage and DSM generated were comparatively analyzed using aerial and TLS data. Accuracy comparison analysis proceeded as follows. First, we used Ortho mosaic image to compare the check point with a certain area. In addition, vertical errors of camera DSM were compared and analyzed based on TLS. In this study, we propose and evaluate the feasibility of UAV photogrammetry which can acquire 3 - D spatial information at low cost in a construction site.

농촌지역 토지이용에 따른 인구 변화

정호현 ( Hohyun Jeong ),정남수 ( Namsu Jung ) 한국농공학회 2009 한국농공학회 학술대회초록집 Vol.2009 No.-

1960년대 국내 도시화율은 20% 수준에서 2007년도 현재 90.5%로 세계 최고수준에 달하고 있다(통계청, 2007). 도시화가 진행됨에 따라 농촌의 토지이용 또한 많은 변화를 겪고 있으며 토지는 인간과 자연의 조형물로서 토지의 이용은 한 지역의 인구 구조 및 형태, 산업 구조를 판단할 수 있는 하나의 척도가 된다(최운식, 1998). 출산율 저하와 기대수명의 증가에 따라 국내의 인구는 외부충격에 취약한 형태로 바뀌고 있는데 특히 농촌지역은 교육 및 노동인력의 유출이 심화됨에 따라 급격한 노령화가 진행되어 정주권의 악화 및 각종 개발 사업의 시행 시 지역개발의 효과가 미비한 실정이다. 2018년에는 노인인구 비율이 14%이상이 되어 고령사회에 진입하게 될 것으로 예상하고 있다 (통계청, 2009). 토지이용의 변화는 시도와 같은 광역단위 뿐만 아니라 시군 또는 읍면과 같은 지역에서도 자산가치 변화 등 지가변동에 따른 변화를 주도하므로 토지이용에 따른 인구의 변화를 파악한다면 향후 농촌지역의 토지이용을 적절히 관리함으로써 농촌의 유지와 지속가능한 성장에 기여할 수 있을 것으로 기대된다. 본 연구에서는 통계자료를 바탕으로 농촌 지역 토지이용과 인구 변화에 관하여 살펴보고자 한다. 연구지역은 예산군으로 선정하였다. 예산군은 충청남도의 북서부 지역 남북간 중간지역에 위치하며 북동쪽에는 천안시(38.8km), 아산시(22.2km)가 소재하는 지역으로 서해안 고속도로와 대전-당진간 고속도로가 부근에 위치하고 철도로는 장항선이 지나가고 있어 교통의 요충지로 행정의 중심지인 예산읍을 포함하여 2개읍, 10개면으로 이루어진 전형적인 농촌지역이다. 분석결과, 예산군의 토지 이용현황은 2000년 기준으로 논 189㎢(34.8%), 과수원 12㎢(2.1%), 임야 253(46.7%), 대지 15㎢(2.7%), 도로 15㎢(2.7%), 수역 40㎢(7.3%), 유지 11㎢(2%)에서 2005년도에는 논 187㎢ (34.4%), 과수원 11㎢(2.1%), 임야 252㎢(46.4%), 대지 15㎢(2.8%), 도로 15㎢(2.8%), 수역 40㎢(7.4%), 유지 11㎢(1.9%)로 논이 0.4%, 임야 0.3% 줄어들었으며, 대지, 도로, 수역이 각각 0.1%씩 증가하였다. 인구현황은 2000년도 기준으로 총인구 101,692명(남:50,919, 여:50,773)에서 2005년에는 총인구 91,452명(남:45,990, 여:45,462)로 약 10% 줄어들었음을 확인하였다. 인구구조는 2000년도 기준으로 15세 이하 16,700명, 노동인구(15세~69세) 76,556명, 노인인구(70세 이상) 8,436명에서 2005년에는 15세 이하 13,183명, 노동인구(15세~69세) 67,627명, 노인인구(70세 이상) 10,642명으로 15세 이하는 2%, 노동인구는 2% 줄어들었으며 반면에 노인 인구는 4% 늘어나 노령화가 진행되었다.

산지이용 실태조사(2011년)

정호현(Hohyun Jeong),이현승(Hyunseung Lee) 한국지역사회생활과학회 2013 한국지역사회생활과학회 학술대회 자료집 Vol.2013 No.4

Determination of Ambroxol in Human Plasma by Liquid Chromatography Tandem Mass Spectrometry

Han Sang Beom,Lee Hee Joo,Choi Mee Hee,Kwon Jeong Mi,Yoo Jeong-Yeon,Kim Hohyun 대한약학회 2002 大韓藥學會 總會 및 學術大會 Vol.2002 No.1