Protective Effect of Baicalin Against Carbon Tetrachloride–Induced Acute Hepatic Injury in Mice

Park, Sang-Won,Lee, Chan-Ho,Kim, Yeong Shik,Kang, Sam Sik,Jeon, Su Jin,Son, Kun Ho,Lee, Sun-Mee The Japanese Pharmacological Society 2008 JOURNAL OF PHARMACOLOGICAL SCIENCES Vol.106 No.1

<P>This study examined the effects of baicalin, a bioactive flavonoid isolated from <I>Scutellariae Radix</I>, on carbon tetrachloride (CCl<SUB>4</SUB>)-induced liver injury. Mice were treated intraperitoneally with 0.5 ml/kg CCl<SUB>4</SUB> and different groups of animals received 25, 50, 100, and 200 mg/kg baicalin. At 24 h after the CCl<SUB>4</SUB> treatment, the level of serum aminotransferases and lipid peroxidation was significantly elevated, whereas the hepatic glutathione content was decreased. These changes were attenuated by baicalin. The histological studies showed that baicalin inhibited the portal inflammation, centrizonal necrosis, and Kupffer cell hyperplasia, which are the three most common characteristics of CCl<SUB>4</SUB>-induced liver damage. The serum level and mRNA expression of tumor necrosis factor-α were markedly increased by the CCl<SUB>4</SUB> treatment but suppressed by baicalin. The mRNA and protein expression levels of inducible nitric oxide synthase and heme oxygenase-1 increased significantly at 24 h after the CCl<SUB>4</SUB> treatment. Baicalin attenuated the increase in the protein and gene expression of inducible nitric oxide synthase but augmented the increase in those of heme oxygenase-1. These findings suggest that baicalin protects hepatocytes from the oxidative damage caused by CCl<SUB>4</SUB>, and this protection is likely due to the induction of HO-1 expression and the inhibition of the proinflammatory mediators.</P>

Ortho-Chlorobenzylidene Malononitrile(CS) 이 흰쥐 피부조직에 미치는 조직학적 영향에 관한 연구

이우용,최봉근,김잉곤,엄기일,정호삼,정호삼,류재만 한양대학교 의과대학 1998 한양의대 학술지 Vol.18 No.1

This study was undertaken to describe the fine structural changes of cytoplasmic organelles in epidermal cells, and the changes of volume and localization of collagen type Ⅰ in the dermis of rat skin by CS(Ortho-chlorobenzylidene malononitrile) application. The experimental animals, 100∼150gm of body weight rats(Sprague-Dawley strain), were sacrificed at 12 hours, 24 hours, 3 day and 5 day after CS application on the hair-shaved back skin. The specimens were prepared for ultrastructural findings, ultrathin sections were stained with uranyl acetate and lead citrate double stain and observed with Hitachi-600 electron microscrope. The others were incubated with rabbit anti rat type Ⅰ collagen antibody as primary antibody, and Goat anti rabbit IgG antibodies as secondary antibody And advidin-biotin-conjugated(ABC) peroxidase procedure was used. All of specimens for immune reactions were observed with light microscope. The results obtained were as follows. 1 Keratinocytes appeared fully necrotized after 3 days of CS application. The epidermis showed degeneration of basal lamina, enlargement of intercellular spaces and necrotic keratinocytes with vacuole and vesicles. And large keratohyalin granules were seen in the shrunken epidermal cells. 2 At 3 or 5 day after CS treatment, the normal cytoplasmic organelles of keratinocytes began to appear on the basal and prickel cells. 3 At 12 hour an 24 hour after CS treatment. The immune reaction of type Ⅰ collagen showed weak reaction in reticular layer of dermis. But at 3 day and 5 day after CS treatment, moderate immune reaction were seen in throughout the dermis. These results suggest that CS application to rak skin may induce changes of keratinocytes and reductive changes of type Ⅰ collagen in the dermis.

병렬 GHT 모델을 이용한 다중형태인식

金鎭浩,趙三模,高昌林 慶一大學校 1993 論文集 Vol.9 No.1

A parallel GHT model is introduced which can detect multiple shapes simultaneously with an efficient use of Hough space and modification of its accumulation method. In this method, we separated the Hough space into two and accumulated the evidences of reference points in two directions of up and down. The proposed model is implemented on the Transputer system and the resulted system proves to have simultaneous rotation-and scaling-invariant detection capability for four different shapes.

흰쥐 肝臟의 組織發生에 관한 組織化學的 및 電子顯微鏡的 硏究

鄭鎬三,李圭植,張世榮 한양대학교 의과대학 1985 한양의대 학술지 Vol.5 No.1

Numerous workers have intensively studied for physological and histochemical aspects on the histogenesis and differentiation of mammalian livers focusing attention on its hematopoietic function. In early fetal life hepatic diverticulum formed caudal part of the foregut in separated into two parts by the mesodermal septum each being developed into primordia later. The author has undertaken histological and histochemical studies to persue the morphological and functional changes to occur in hepatic lobules during development and differential period. The pregnant albino rats, Wistar strain, were sacrificed every day starting from 15th to 20th day of gestation. The specimens obtained from the fetal liver were fixed with 10% formalin and stained with hematoxylin-eosin to observe the morphological development and differentiation of the liver. The activities of acid and alkaline phosphatase were observed by the Gomori's methods(1931, 1941) and ATPase activity by the Wachstein-Meisel's method(1957). And also another liver specimens fixed with 4% glutaraldehyde in 0.1M cacodylate buffer(pH 7.4) at 4℃ by the Eranko's method(1952) were examined with electron microscope to investigate phosphatase activities in the hepatic parenchymal cells. The results were as follows. 1. In the liver of the 15th day of the fetus, undifferentiated hepatic parenchymal cells were constituted and a few nucleated erythocytes were spread at the intercellular region, and at the 16th and 17th day of the fetus a number of hematocytoblast were distributed and primitive hepatic cord and hepatic sinusoid were formed. 2. In the Liver of the 18th, 19th and 20th day of the fetus, hematopoietic cells were reduced but the differentiation of he hepatic parenchymal cells, hepatic cord and hepatic sinusoid were observed. 3. The activity of the acid phosphatase increased from the 17th to 20th day of the fetus. High activity of the enzyme at the peripheral and central zone and trace activity at the lysosome and rough endoplasmic reticulum were observed. 4. Moderate activity of the alkaline phosphatase was observed in the liver of the 19th and 20th day of the fetus at the nuclear membrane of the parenchymal cell and at the villi of the bile canaliculi. 5. The activity of the adenosine triphosphatase was observed in the liver of the 18th day of the fetus, and positive activity at the plasma membrane and weak activity at the rough endoplasmic reticulum were observed at the 19th and 20th day of the fetus, respectively.

치수절단 후 접착레진 도포에 대한 잔존치수조직 반응에 관한 연구

임성삼,박동성,손호현 大韓齒科保存學會 1997 Restorative Dentistry & Endodontics Vol.22 No.2

This study was performed to observe the histopathological response to the bonding resin directly applied on the remaining pulp tissuse. 40 teeth from 3 adult dogs were pulpotomized with a sterile round but and sharp excarvater. In the control group, Ca(OH)_2 powder was applied on the pulp tissue and the cavities were sealed with IRM cement. In the experimental group 1, Superbond C&B was applied on the remaining pulp and the cavities conditioned with 10-3 solution were filled with the mixture of the MMA liquid, PMMA powder and Catalyst. Multi-purpose adhesive was used on the remaining pulp tissue in the experimental group 2 and Z-100 was filled in the cavities. In the experimental group 3, Clearfil photobond applied and directly photo=cured on the pulp tissue, then the cavitieswere treated wit CA agent (10% citric acid and 20% CaCl_2 aqueous solution) for 20 seconds, washed and applied with Clearfil photobond then filled with Protect liner. The experimental animals were sacrified at the 1st, 2nd, and 4th week. The specimens were routinely processed and stained with H-E for light microscopic observation. The results were as followed : 1. In the experimental group 1, the number and characteristics of the dentin bridge formation case was similar to those in the control group and less cases were observed in the experimental group 2 and 3 than experimental group 3. The inflammatory response in experimental group 1 was less than that in the control group at 1st week but there had been little difference at between 2nd and 4th week. 2. The number of the dentin bridge in experimental group 2 was less than that in control group and experimental group 1. The inflammatory response of the experimental group 1 was similar to that of experimental group 1 but less than that of the control group. A number of bleeding and vascular congestion were observed. The least inflammatory response was seen in the experimental group 2 among all groups. 3. In the experimental group 3. one case of the dentin bridge formation was observed and that was the same as that in the experimental group 2 but smaller than that of the control and experimental group 1. The inflammatory response of the experimental group 3 was least at the 1st week and most at the 4th week in the all group.

성견에서 Super EBA, Ketac Silver, MTA와 Emdogain을 이용한 치근분지부 천공치유에 관한 연구

임성삼,손호현,백승호 大韓齒科保存學會 2000 Restorative Dentistry & Endodontics Vol.25 No.2

地方工業立地를 통한 農村地域人口 定着에 관한 硏究 : 晋州一帶 工場從業員 調査를 中心으로 Based on the Result of Questionaire Survey to the Factory Employed in Chinju and its Vicinity

李成浩,崔三鎔 부산대학교 사회과학대학 1985 社會科學論叢 Vol.4 No.2

Recently, the drastic decrease of population in the rural areas has emerged as a serious issue. This trend, stemmed directly from the phenomena of urbanization following the high rise industrialization, can be to some extent blamed on the existing direction of both the economic and regional development policy. Namely, the development scheme, based on the manufacturing-led growth center model Korea has pursued since 1960s, has brought about the relative stagnation in the rural part of the nation, causing naturally the outmigration of rural population into the urban areas. The rural decline ensued from the successive population drain entails various aspects of the negative impacts, such as the wide range of interregional income disparities which may disrupt the national consensus of the nationhood and raise the dual structure in the physical land space. This paper, viewed from the above problem statement, is aimed at analyzing the effect of the case of inducing the factories into the rural areas as a development strategy for the efficient rural settlement of population and also investigating the status of the present rural manufacturing to identify what is required to support such project. The manufacturing employed from 10 factories in Chinju and its vicinity, consisting of 573 people, was sampled for conducting the case study. Questionnaire survey sheets were given to each respondent to obtain the relevant ranges of socio-economic data relating to his or her work-place and its condition; survey items contain such information as family status, dwelling and its tenure, education, income, transport means for journey to work, and conditions of workplace and jobs. Main findings confirmed through the analysis can be summarized as follows. First, as the result of the analysis on the past trend of population movement, it is identified that the phenomena of the increase and decrease of population has a close relation to the regional distribution of industries. Second, it is revealed that the male is aged higher than the female; the married has the higher portion than the non-married. Third, it is shown that most of the manufacturing workers have the low level of monthly income and educational background. Fourth, it is indicated that some variables such as the present address, the birth place, the place of final education, and the dwelling place before taking jobs have intimate relationships with each other; it also appears that in view of no evidence of taking the present job to be a factor of population movement, the setting up of factories in the rural areas can't be a component of population migration. Fifth, it is found that the level of satisfaction of the present job varies on the basis of job training experience and the level of monthly income. Accordingly, in order to encourage rural people to take a job in the present place, it is apparently necessary for creating the job training programs for procurement of the job-related technical license as well as taking some policy measure to improve the existing wage systems. Sixth, it is also found that the rural area has still the potentiality to provide quality of labor forces for its own manufacturing operation, that is an important factor for the rural base factories to be managed in the better direction. Seventh, it is identified that most of the respondents have the commuting distance of 12km or less and the time range of one hour or less, for journey to work; the best desirous mean of transport for factory workers can be the commuting by the walk, the bicycle-riding, and the commuting bus provided by the firm. Eighth, in conclusion it should be pointed out that the success of population settlement in the rural area through the localized manufacturing bases depends upon the common effort by the rural residents, the entrepreneurs, the central government, together with precarious choice of the relevant establishments at the proper scales of the operating units on which procurement of raw materials and search for markets are relied.

치근단 및 치수병변 환자에서의 TNF-α와 β, IL-1β및 TGF-β1 의 수준과 근관내 특정 black pigmented bacteria와의 연관성에 관한 연구

변호영,임성삼,박동성 大韓齒科保存學會 1999 Restorative Dentistry & Endodontics Vol.24 No.1

응급실에서 시행한 상용심전도의 효용성에 대한 고찰

도호석,이창현,이삼범,도병수 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Study objectives : To determine whether routine performance of an ECG could have been safely avoided in a subset of ED(emergency department) patients admitted to a university hospital. Design : Retrospective consecutive case series. Setting : University teaching hospital. Type of participants : All patients admitted through Yeungnam university hospital during a three month period. Methods and interventions : Acceptable indications for an admission ECG were prospectively developed. Charts of all patients were reviewed to determine whether any of these were present, whether an admission ECG performed, and whether an admission ECG resulted in a change in patients management or outcome. Measurements and main result : There were 984 admissions through ED during the study period. Of these 608 patients had an ECG performed at ED. Of the 608 patients 140(23.0%) had at least one acceptable indication and 468(77.0%) had no for an ECG. Among the 140 who had an indication for an ECG at ED, 56(40.0%) patients ECG were interpreted abnormal (95% confidence interval[CI], 32%∼48%). Among the 468 who had no indications, only 3(0.64%) patients ECG were interpreted abnormal (95% CI, 0%∼1.36%) and the ECG result in a change in management, but no change in outcome (95% CI, 0%∼0.64%). Conclusion : The routine or admission ECG could have been avoided in no acceptable ED indication. We should have to try continuously for making acceptable criteria of ECG in ED of Korea, and then we have to prevent needless ECG and cardiac work up that increasing the cost and evacuation time from ED.

Mitomycin이 Mouse의 肝細胞 내 Pyronin 好性顆粒에 미치는 影響

鄭鎬三,李圭植,白斗鎭 한양대학교 의과대학 1985 한양의대 학술지 Vol.5 No.1

Mitomycin, one of the mitosane antibiotics, is isolated from Streptomyces caespitosus which has anticancer effect. It is well known that mitomycin inhibites DNA synthesis in mammalian cells. The major machanism accounting for the inhibitory effect of mitomycin is interference of the template activities of the DNA by cross linking to DNA double helix. The auther has investigated the effect of mitomycin on the liver of mice, observing the distribution and disaggregation of the pyroninophilic granules in the hepatic parenchymal cells. A total of 52 healthy mice of ICR strain (the weight 20g) were divided into two groups; The control and the mitomycin treated groups. All experimental animals were given food and water ad libitum. The animals of the mitomycin treated groups were administered 4mg per kg of body weight of mitomycin diluted in the water for injection by intraperitoneal injection. The animals of the MC treated groups were sacrified at intervals of 6, 12, 24 and 36 hours after administration of mitomycin Histochemical preparations were stained with the methyl green-pyronin method for the pyroninophilic granules in the hepatic parenchymal cells. A marked reduction or disappearance of the pyroninophilic granules were observed in the entire hepatic lobule at 24 hours after administration of mitomycin. Consequently, it is suggested that mitomycin causes the loss of pyroninophilic granules in the hepatic parenchymal cells of the liver in the mitomycin treated mice.