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Soh, Ju-Ryoun,Kim, Nam-Seok,Oh, Chan-Ho,Oh, Suk-Heung,Cha, Youn-Soo The Korean Society of Food Science and Nutrition 2010 Preventive Nutrition and Food Science Vol.15 No.3
This study evaluated the effects of carnitine and/or GABA supplementation on immune function, lipid profiles and some vitamins in mice chronically administered alcohol. BALB/c mice were fed with either AIN-76 diet (N), control diet plus alcohol (4 g/kg bw, E), E plus 0.5 g/kg bw carnitine (EC), E plus 0.5 g/kg bw GABA (EG), or E plus 0.5 g/kg bw carnitine plus 0.5 g/kg bw GABA (ECG) for 6 weeks. Administrations of the carnitine and/or GABA prevented alcohol-induced increases in triglyceride concentrations in serum and liver. However, there was no difference among the supplemented groups. Serum vitamin E concentration was higher in mice supplemented with EC and EG, but not in mice given ECG. Phagocytic activity of peritoneal macrophages was increased in EG group compared with E group. The subpopulations of murine splenocyte's TH cells were increased significantly in EC and ECG groups. These data suggest that immune function, lipid profiles and some immune-related lipid soluble vitamins were positively changed by supplementation of carnitine or GABA, but do not show any synergistic effect of mixed supplementation.
Ho-Seob Soh,Young-Hee Han,Gee-Young Lee,Jae-Wook Lim,Bu-Young Yi,Youn-Hyung Lee,Geun-Won Choi,Young-Doo Park 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.1
The objective of this study was to generate a pest-resistant Chrysanthemum morifolium by transforming crylAc gene, which has resistant to pests belonging to Noctuidae family that causes problems that damage to leaves and flowers during cultivation, into Chrysanthemum morifolium. For plant regeneration, standard type Jinba (JB) and Suhonochikara Yellow (SY) cultivars cultured on the medium supplemented with NAA 1.0 ㎎ㆍℓ<SUP>-1</SUP> and BA 2.0 ㎎ㆍℓ<SUP>-1</SUP> combination and spray type EuroYellow (EY) cultivar supplemented with NAA 1.0 ㎎ㆍℓ<SUP>-1</SUP> and BA 1.0 ㎎ㆍℓ<SUP>-1</SUP> combination showed the highest regeneration rates, respectively. When selectable reagents were added to selective medium for leaf segment culture, regeneration was inhibited on concentrations of above 0.5 ㎎ㆍℓ<SUP>-1</SUP>, 5.0 ㎎ㆍℓ<SUP>-1</SUP> and 10.0 ㎎ㆍℓ<SUP>-1</SUP> hygromycin for SY, EY and JB cultivars, respectively, and 0.5 ㎎ㆍℓ<SUP>-1</SUP> phosphinothricin for all JB, SY and EY cultivars. When transgenic Chrysanthemum morifolium transformed by pGR-BT vector was analyzed by PCR, the expected size of 479 bp was observed on 29 JB lines, 13 SY lines and 5 EY lines. For pMJ-BT vector, 3 JB lines, 3 SY lines, and 1 EY line were selected as transformants. Finally, transformed lines selected by PCR analyses were inoculated with Spodoptera litura (tobacco cutworm) and showed that there was no larvae growth from 6 JB lines, 1 SY line and 1 EY line.