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        Bacterial Species and Biochemical Characteristic Investigations of Nostoc flagelliforme Concentrates during its Storage

        ( Lifang Yue ),( Hexin Lv ),( Jing Zhen ),( Shengping Jiang ),( Shiru Jia ),( Shigang Shen ),( Lu Gao ),( Yujie Dai ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.4

        Preservation of fresh algae plays an important role in algae seed subculture and aquaculture. The determination and examination of the changes of cell viability, composition, and bacterial species during storage would help to take suitable preservation methods to prolong the preservation time of fresh algae. Nostoc flagelliforme is a kind of edible cyanobacterium with important herbal and dietary values. This article investigated the changes of bacterial species and biochemical characteristics of fresh N. flagelliforme concentrate during natural storage. It was found that the viability of cells decreased along with the storage time. Fourteen bacteria strains in the algae concentrate were identified by PCR-DGGE and were grouped into four phyla, including Cyanobacteria, Firmicutes, Proteobacteria, and Bacteroidetes. Among them, Enterococcus viikkiensis may be a concern in the preservation. Eleven volatile organic compounds were identified from N. flagelliforme cells, in which geosmin could be treated as an indicator of the freshness of N. flagelliforme. The occurrence of indole compound may be an indicator of the degradation of cells.

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        Study on Multi-Field Coupled Evolution Mechanism of Laser Irradiated 40Cr Steel Quenching Process Based on Phase Change Induced Plasticity

        Chang Li,Hexin Gao,Xinxue Chen,Zhaotai Liu,Xing Han 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.8

        40Cr is essential to steel for the manufacture of hardened gears, and optimizing its laser quenching process will be of greatsignificance for gear wear and life extension. However, the complete reliance on the experimental trial-and-error approachdoes not effectively reveal the mechanism of the evolution of the laser quenching process in disk laser. Numerical simulationprovides an effective way to obtain the transient evolution of the laser quenching process. In this paper, the temperaturechanges physical parameters of the quenched material are calculated based on the CALPHAD method. Establish a thermoelastic–plastic multi-field coupling model for the laser hardening process of the disk laser 40Cr gear steel. Perform thenumerical calculations on the transient temperature, martensite phase fraction, and the transformation-induced plasticitystress evolution of the 40Cr laser hardening process. Focus on the mechanism of the mutual coupling between phase changebehavior and plastic strain. Calculations show that there is heat accumulation during quenching, with a peak temperatureof 1448 K. Plastic stress has a “hump” distribution, with a peak value of 834 MPa, which is 1.25 mm from the center ofthe focal point. The experimental analyses of the 40Cr gear steel quenching microstructure and hardness distribution stateby the Axioskop2 scanning electron microscope, the KEYENCE VH-Z100R super depth of field 3D microscope, and theQ10M microhardness tester reveal the mechanical behavior of the material and the phase hardening law during the quenchingprocess. That provided an essential theoretical basis for effective prediction on quenching residual stress and optimizingindustrial production parameters.

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