경골어류 카라신과 glow-light tetra의 난자형성과정

이규재,장병수,등영건,김동희 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.4

조기어강 (Actinopterygii), 카라신목 (Characiformes), 카라신과(Characidae)에 속하는 담수산 경골어류에 속하는 Glow-light tetra (Hemigrammus erythrozonus)의 난자형성과정을 다른 어류와의 차이점을 확인하기 위하여 광학현미경으로 관찰한 결과 난원세포의 세포질은 호염기성이었고 핵 내에 다수의 이형염색질들이 분포하고 있었다. 제1난모세포의 경우 난황포가 단지 난세포 가장자리에만 배열되어 있었고 난막의 형성은 관찰되지 않았다. 제2난모세포에서는 난막이 형성되었고 제1난모세포에 비해서 난황포가 점점 핵 쪽으로 증식된 경향을 보였다. 발생이 진행됨에 따라서 호염기성 물질들은 점점 감소하는 경향을 보였고 후에 난막 주위에만 국한적으로 분포하였으며, 난막의 두께와 난자의 크기는 점점 증가되었다. 이상과 같이 glow-light tetra의 난자형성과정은 난세포의 크기 증가, 난황낭의 축적, 염기성 물질의 감소, 난막발달 및 두께의 증가로 요약될 수 있으며 일반적인 다른 과의 담수경골어류와 큰 차이는 없는 것으로 확인되었다. 본 연구는 광학현미경상의 조직학적 결과이므로 앞으로 정확한 난자형성과정을 단계적으로 확인하기 위한 투과전자현미경을 이용한 미세구조적 연구가 필요하다. Glow-light tetra, Hemigrammus erythrozonus is a teleost belonging to Characidae. The natural habitate of this fish is the wild in the Essequibo river, Guyana and South America. The oogenesis of glow-light tetra was investigated by light microscopy to compare with those of other families. A pair of ovary was located between swim bladder and intestines. The ovary was of white color and ellipsoidal shape with the major axis 11 mm and the minor axis 4 mm. Cytoplasm of oogonia was basophilic and many nucleoli were located at inside of nuclear membrane. In primary oocytes, yolk vesicles were distributed only in the marginal area and egg envelope was not formed on the outside of an egg. In secondary oocytes, the egg envelope was formed and yolk vesicles in the cytoplasm were increased than the earlier stage. The amount of basophilic substance was decreased. In case of matured egg, thickness of egg envelope and size of egg were increased, basophilic substance was distributed in only around the egg envelope. The yolk vesicles were changed to yolk mass in accordance with development. In conclusion, the oogenesis of glow-light tetra, Hemigrammus erythrozonus was characterized by the increase in cell size, the formation and accumulation of yolk, and the decrease of basophilia a in the cytoplasm. The oogenesis of glowlight tetra seems to share common patterns in Characidae, teleost and have a similar pattern with other teleost.

전해환원수 음용이 마우스의 혈액과 장기조직에 미치는 영향

정한석,김동희,윤양숙,등영건,장병수,이규재 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.4

본 연구는 전해환원수 음용에 대한 안전성을 조사하기 위하여 실험동물을 이용하여 사망률 확인, 체중 비교, 혈액의 생화학적 변화, 염증, 면역세포의 비교, 내부 장기의 형태적 변화, 장기의 무게 변화 및 조직학적 검사를 통하여 전해환원수가 생체에 미치는 영향을 확인한 결과 다음과 같은 결론을 얻었다. 전해환원수를 급이한 실험군에서 사망, 행동 및 외형 이상 유무 관찰 결과 아무런 증상이 나타나지 않았으며, 체중은 감소하는 경향을 보였다. 실험군에서 혈액의 생화학적요소인 K^(+), Cl^(-),Ca^(2+), GPT, GOT, CRE 및 ALP에서는 유의한 수준의 변화가 관찰되지 않았으나 Na^(+)와 BUN에서는 실험군이 대조군에 비해 더 감소되었다. 혈액 내 면역세포를 비교한 결과 호염기구, 호산성구 및 단핵구 등은 큰 차이가 없으며, 실험군에서 호중구는 감소하였고, 림프구는 증가하는 경향을 보였다. 또한 위, 소장, 심장 및 간을 조직학적으로 관찰한 결과 모든 장기에서 병리적 이상 소견은 관찰되지 않았다. 따라서 본 연구를 통하여 전해환원수는 생체에서 큰 문제를 유발시키지 않는 안전한 물임을 알 수 있었고 국내에서 전해환원수에 대한 연구가 거의 없는 실정이기 때문에 전해환원수의 안전성을 입증하기 위한 기초자료 마련 및 제공에 의의가 있다고 할 수 있다. The main purpose of this study is to investigate the effects of electrolyzed reduced water (ERW) on blood components and electrolytes, as well as elected tissues on mice. The mice were supplied either tap water (control group) and ERW (experimental group) for two months. There were no significant different between two groups anatomically and physiologically. In the blood electrolyte study, the experimental group had less Na and BUN compared to the control group. In the blood component study, the experimental group had less neutrophiles and the control group had more lymphocytes. In histological study, no tissue changes were noticed in various organs, including the stomach, small intestine, heart, and liver tissues. In conclusion, ERW has no noticable side affects on blood and organ tissues, and might be safe to a living body.

Role of accidental torsion in seismic reliability assessment for steel buildings

Heui-Yung Chang,Jung-Yu Chen,Chu-Chieh Jay Lin,Ker-Chun Lin 국제구조공학회 2009 Steel and Composite Structures, An International J Vol.9 No.5

This study investigates the role of accidental torsion in seismic reliability assessment. The analyzed structures are regular 6-story and 20-story steel office buildings. The eccentricity in a floor plan was simulated by shifting the mass from the centroid by 5% of the dimension normal to earthquake shaking. The eccentricity along building heights was replicated by Latin hypercube sampling. The fragilities for immediate occupancy and life safety were evaluated using 0.7% and 2.5% inter-story drift limits. Two limit-state probabilities and the corresponding earthquake intensities were compared. The effect of ignoring accidental torsion and the use of code accidental eccentricity were also assessed. The results show that accidental torsion may influence differently the structural reliability and limit-state PGAs. In terms of structural reliability, significant differences in the probability of failure are obtained depending on whether accidental torsion is considered or not. In terms of limit-state PGAs, accidental torsion does not have a significant effect. In detail, ignoring accidental torsion leads to underestimates in low-rise buildings and at small drift limits. On the other hand, the use of code accidental eccentricity gives conservative estimates, especially in high-rise buildings at small drift limits.

A study on behavior of steel joints that combine high-strength bolts and fillet welds

Heui-Yung Chang,Ching-Yu Yeh 국제구조공학회 2019 Steel and Composite Structures, An International J Vol.31 No.4

In recent years, considerable attention has been paid to the research and development of high-strength steel plates, with particular emphasis on the enhancement of the seismic resistance of buildings and bridges. Many efforts have also been undertaken to improve the properties of high-strength bolts and weld materials. However, there are still different opinions on steel joints that combine high-strength bolts and fillet welds. Therefore, it is necessary to verify the design specifications and guidelines, especially for newly developed 1,400-MPa high-strength bolts, 570-MPa steel plates, and weld materials. This paper presents the results of literature reviews and experimental investigations. Test parameters include bolt strengths, weld orientations, and their combinations. The results show that advances in steel materials have increased the plastic deformation capacities of steel welds. That allows combination joints to gain their maximum strength before the welds have fracture failures. When in combination with longitudinal welds, high-strength bolts slip, come in contact with cover plates, and develop greater bearing strength before the joints reach their maximum strength. However, in the case of combinations with transverse welds, changes in crack angles cause the welds to provide additional strength. The combination joints can therefore develop strength greater than estimated by adding the strength of bolted joints in proportion to those of welded joints. Consequently, using the slip resistance as the available strength of high-strength bolts is recommended. That ensures a margin of safety in the strength design of combination joints.

한국형 출혈열 환자에서 병기에 따른 혈중 Atrial Natriuretic Polypeptide 농도의 변화

박정의(Jeong Euy Park),송치욱(Chi Wook Song),송관규(Gwan Gyu Song),표회정(Heui Jung Pyo),박승철(Seung Chull Park),이창홍(Chang Hong Lee),김대원(Dae Won Kim),이갑노(Kap Ro Lee),양영선(Yung Sun Yang),황흥곤(Heung Kon Hwang),문정식(Jung 대한내과학회 1987 대한내과학회지 Vol.33 No.6

N/A The plasma atrial naturiuretic polypeptide (ANP) was repeatedly measured in each clinical phases in 20 patients (20.8±7.2yr) with Korean Hemorrhagic Fever (KHF). The study purpose was to see whether there is any relationship between the clinical course of KHF and the changes of plasma level of ANP. In three patients the plasma ANP could be repeatedly measured from the early oliguric to diruetic phases. In these three patients the plasma level of renin activity and aldosterone were very high in the early oliguric phase. Then the plasma renin activity and aldosterone level decreased steeply in remarkable degree to near normal level while the patient is still in oliguric phase and stayed at this level during the diuretic phase. The plasma ANP level was low (30-80pg/ml) in the early oliguric phase. Then with the sudden remarkable decrease of plasma renin activity and aldosterone the plasma ANP increased rapidly to high level (230-280pg/ml), then was decreased to near normal level (90-190pg/ml) making a narrow peaked plasma ANP concentration curve, and then the level stayed at near normal level during the diuretic phase. Around the time when the plasma renin activity and aldosterone concentrations were decreased to their low level and the plasma ANP was increased to it's peak level the oliguric phase was changed to the diuretic phase. The changes of plasma cortisol level followed the pattern of changes of plasma renin and aldosterone. It is possible that the increase of plasma ANP level might have had some role in the initiation of diuretic phase in these patients. It is not certain whether these changes are the unique changes seen only in patients with KHF or the changes seen also in patients with acute renal failure of other causes.

Corrosion Behaviors of Structural Materialsin High Temperature S-CO2 Environments

( Ho Jung Lee ),( Hyunm Yung Kim ),( Chang Heui Jang ) 한국부식방식학회(구 한국부식학회) 2014 Corrosion Science and Technology Vol.13 No.2

The isothermal corrosion tests of several types of stainless steels, Ni-based alloys, and ferritic-martensitic steels (FMS) were carried out at the temperature of 550 and 650 oC in SFR S-CO2 environment (200 bar) for 1000 h. The weight gain was greater in the order of FMSs, stainless steels, and Ni-based alloys. For the FMSs (Fe-based with low Cr content), a thick outer Fe oxide, a middle (Fe,Cr)-rich oxide, and an inner (Cr,Fe)-rich oxide were formed. They showed significant weight gains at both 550 and 650 oC. In the case of austenitic stainless steels (Fe-based) such as SS 316H and 316LN (18 wt.% Cr), the corrosion resistance was dependent on test temperatures except SS 310S (25 wt.% Cr). After corrosion test at 650 oC, a large increase in weight gain was observed with the formation of outer thick Fe oxide and inner (Cr,Fe)-rich oxide. However, at 550 oC, a thin Cr-rich oxide was mainly developed along with partially distributed small and nodular shaped Fe oxides. Meanwhile, for the Ni-based alloys (16-28 wt.% Cr), a very thin Cr-rich oxide was developed at both test temperatures. The superior corrosion resistance of high Cr or Ni-based alloys in the high temperature S-CO2 environment was attributed to the formation of thin Cr-rich oxide on the surface of the materials.

당뇨병성 신경병증 진단에서 잔여 잠시의 의의

서정,박주현,정경희,장준영,최진홍,김용석 大韓再活醫學會 1998 Annals of Rehabilitation Medicine Vol.22 No.6

N-benzyl-N-methyldecan-1-amine, a phenylamine derivative isolated from garlic cloves, induces G2/M phase arrest and apoptosis in U937 human leukemia cells.

Jeong, Jin-Woo,Park, Sejin,Park, Cheol,Chang, Young-Chae,Moon, Dong-Oh,Kim, Sung Ok,Kim, Gi-Young,Cha, Hee-Jae,Kim, Heui-Soo,Choi, Young-Whan,Kim, Wun-Jae,Yoo, Young Hyun,Choi, Yung Hyun National Hellenic Research Foundation 2014 ONCOLOGY REPORTS Vol.32 No.1

<P>Epidemiological studies indicate that components of garlic (Allium sativum) have anti-proliferative effects against various types of cancer. In the present study, we investigated the effect of newly isolated phenylamine derivative N-benzyl-N-methyldecan-1-amine (NBNMA) from garlic cloves on the inhibition of the growth and apoptosis of human leukemia U937 cells and its potential anticancer mechanism. NBNMA exhibited an antiproliferative effect in U937 cells by inducing cell cycle arrest at the G2/M phase and apoptotic cell death. Western blot analyses revealed that NBNMA decreased the expression of the regulator genes of G2/M phase progression, cyclin dependent kinase (Cdk) 2 and Cdc2 and elevated the expression of the Cdk inhibitor p21WAF1/CIP1 in a p53-independent manner. In addition, NBNMA activated caspase-8 and caspase-9, initiator caspases of the extrinsic and intrinsic pathways of apoptosis, respectively, which led to activation of executioner caspase-3 along with degradation of poly(ADP-ribose) polymerase. NBNMA-induced apoptosis was observed in parallel with an increased ratio of pro-apoptotic Bax and Bad/anti-apoptotic Bcl-2 and Bcl-xL, and inhibition of inhibitor of apoptosis protein (IAP) family members XIAP and cIAP-1. Furthermore, NBNMA-treated cells displayed enhanced release of cytochrome c from the mitochondria into the cytosol concomitant with a loss of mitochondrial membrane potential and downregulation of Bid, suggesting that NBNMA-induced apoptosis occurred via the extrinsic and intrinsic apoptotic pathways with a possible link to Bid protein activity between the two pathways. These results indicate that NBNMA has promising potential to become a novel anticancer agent for the treatment of leukemia. We provide new insight into the mechanisms underlying the anticancer effect of NBNMA.</P>

경골어류 황어아과 버들치의 난자형성과정

김동희,장병수,정한석,등영건,김석,이규재,Kim, Dong-Heui,Chang, Byung-Soo,Jung, Han-Suk,Teng, Yung-Chien,Kim, Seok,Lee, Kyu-Jae 한국현미경학회 2009 Applied microscopy Vol.39 No.3

담수산 잉어과(Cyprinidae), 황어아과(Leuciscinae)에 속하는 버들치(Chinese minnow; Rhynchocypris oxycephalus)의 난자형성과정과 성숙난 난막의 미세구조를 광학현미경과 전자현미경으로 관찰한 결과는 다음과 같다. 난원세포의 세포질은 호염기성이었고 핵 내에 다수의 인들이 분포하고 있었다. 제1난모세포의 경우 난황포가 단지 난세포 가장자리에만 배열되어 있었고 난막의 형성은 관찰되지 않았다. 제2난모세포에서는 난막이 형성되었고 제1난모세포에 비해서 난황포가 점점 핵 쪽으로 증식된 경향을 보였다. 발생이 진행됨에 따라서 호염기성 물질들은 점점 감소하는 경향을 보였고 후에 난막 주위에만 국한적으로 분포하였으며, 난막의 두께와 난자의 크기는 점점 증가되었다. 배란된 성숙난 난막의 표면은 미세융모와 같은 구조물들에 의하여 덮혀 있었고 동물극 쪽에 깔떼기 형태의 난문이 발견되었다. 난막의 단면을 확인한 결과 부착성 외층과 섬유상 내층 모두 2층으로 구성되어 있었다. 이상과 같이 버들치의 난자형성과정은 난세포의 크기 증가, 난황낭의 축적, 염기성 물질의 감소, 난막발달 및 두께의 증가로 요약될 수 있으며 이 패턴은 잉어과의 공통적인 특징으로 생각된다. 그러나 배란된 성숙난 난막의 미세구조적 특징들은 이 종만이 가지는 종특이성으로 종을 분류하는 데 이용될 수 있다. Chinese minnow, Rhynchocypris oxycephalus is a teleost belonging to Leuciscinae, Cyprinidae. The oogenesis and ultrastructure of egg envelope in Chinese minnow were investigated by light and electron microscopes. The ovary was of white yellowish and ellipsoidal shape with the major axis 30 mm and the minor axis 7mm. Cytoplasm of oogonia was basophilic and many nucleoli were located at inside of nuclear membrane. In primary oocytes, yolk vesicles were distributed only in the marginal area and egg envelope was not formed on the outside of an egg. In secondary oocytes, the egg envelope was formed and yolk vesicles in the cytoplasm were increased than the earlier stage. The basophilic substance of cytoplasm was changed to acidic. In case of matured egg, thickness of egg envelope and size of egg were increased. The yolk vesicles were changed to yolk mass in accordance with development. The outer surface of egg envelope was covered by microvilli-structures, and had a micropyle on the area of animal pole. Egg envelope consisted with 2 layers, an adhesive outer layer with microvilli-structures and fibrillar inner layer. In conclusion, the oogenesis of Chinese minnow was characterized by the increase in cell size, the formation and accumulation of yolk, and the decrease of basophilic substance in the cytoplasm. The oogenesis of Chinese minnow seems to share common patterns in Cyprinidae, but these ultrastructural unique characters of egg envelope can be utilized in taxonomy of teleost.

경골어류 시클리드과 Cichlasoma managuensis의 수정란 난막 미세구조

김동희,장병수,등영건,김석,주경복,이규재,Kim, Dong-Heui,Chang, Byung-Soo,Teng, Yung-Chien,Kim, Seok,Joo, Kyung-Bok,Lee, Kyu-Jae 한국현미경학회 2009 Applied microscopy Vol.39 No.1

경골어류 시클리드과에 속하는 Cichlasoma managuense의 수정란 난막구조를 광학현미경과 전자현미경을 이용하여 관찰하였다. 수정란은 약황색의 불투명한 부착성 및 침란성란으로 장타원형이었으며 크기는 장축 $1.43{\pm}0.04\;mm$, 단축 $1.85{\pm}0.03\;mm$였다. 수정란의 동물극쪽에서 수정을 위한 정자의 통로로 생각되는 난문이 위치하고 있었고 위란강은 잘 발달되어 있지 않았다. 수정란 난막의 표면에는 부착성 망상구조물들이 덮고 있었고 난문의 형태는 깔때기 형태였다. 수정란 난막의 단면을 관찰한 결과 2층으로 구성되어 있었으며 외층은 부착성 층, 내층은 전자밀도가 서로 다른 $13{\sim}15$층의 층상구조를 하고 있었다. 수정란의 외형은 전형적인 시클리드과에 속하는 다른 어류와 동일한 형태를 하고 있어 과의 특성을 보이며 이들 수정란 난막에서 표면과 단면의 미세구조는 Cichlasoma managuense만이 가지고 있는 종특이성이 될 수 있기 때문에 경골어류의 종을 분류하는데 형태학적 형질로 사용될 수 있을 것으로 사료된다. The ultrastructure of the fertilized egg envelope in Cichlasoma managuensis belonging to Cichlidae were investigated by routine light and electron microscopes. The fertilized eggs of Cichlasoma managuensis was of the light yellow, non-transparent, ellipsoidal, adhesive and nonfloted type. The size of fertilized egg was the major axis $1.92{\pm}0.08\;mm$, the minor axis $1.43{\pm}0.04\;mm$. The egg envelopes have a single micropyle, which is thought to the pathway of sperm in the area of the animal pole. An outer surface of fertilized egg envelope was covered by a adhesive reticular structures and the fertilized egg envelopes consisted of two distinct layers; an outer adhesive layer and an inner layer of $13{\sim}15$ horizontal lower electron density lamellae alternating with interlamellae of higher electron density. The external shape of fertilized egg is common trait of fishes belonging to Cichlidae and these ultrastructural characters of fertilized egg envelope can be utilized in taxonomy of teleost.