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- 저자 : Hema Ramachandran (검색결과 1 건)
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Hema Ramachandran,A. A. Amirul 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.6
Discharging the unrefined glycerine, a by-productfrom biodiesel production is the simplistic solution adoptedfor its management which has led to its price reduction inthe market worldwide and created serious environmentalimpact. Therefore, we have explored the application ofunrefined glycerine pitch as direct fermentative substratein the biosynthesis of novel yellow-pigmented poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)]copolymer by Cupriavidus sp. USMAHM13 through onestagecultivation. Utilization of glycerine pitch (10 g/L)together with 1,4-butanediol (5 g/L) had resulted in thehighest achievement of 2.91 g/L of P(3HB-co-40%4HB)copolymer which was naturally dyed with the yellowpigment through the co-extraction process. Enhancementof 4HB monomer accumulation was also attained throughthe addition of ammonium acetate as nitrogen source. Itwas revealed that utilization of recovered crude glycerinefrom glycerine pitch was more preferred compared to theother recovered components. Utilization of glycerine pitchin the biosynthesis of P(3HB-co-4HB) copolymer wouldnot only contribute to the efficient waste management butalso would promote the development of cost-efficiencymicrobial fermentation.
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