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RISS 인기검색어
- 검색키워드
- 저자 : A. A. Amirul (검색결과 3 건)
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Zuriani R,Vigneswari S,Azizan M. N. M,Majid M. I. A,Amirul A. A 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.3
A rapid quantitative measurement of accumulated polyhydroxyalkanoate (PHA) is essential for rapid monitoring of PHA production by microorganisms. In the present study, a 96-well microplate was used as a high throughput means to measure the fluorescence intensity of the Nile red stained cells containing PHA. The linear correlation obtained between intracellular PHA concentration and the fluorescence intensity represents the potential of the Nile red method employment to determine PHA concentration. The optimal ranges of excitation and emission wavelengths were determined using bacterial cells containing different types of PHAs, of different co-monomers and compositions. Interestingly, in spite of different co-monomers compositions in each PHA, all tested PHAs fluoresced maximally at excitation wavelength between 520 and 550 nm,and emission wavelength between 590 and 630 nm. The developed staining method also had successfully demonstrated a good correlation between the amount of accumulated PHA based on the fluorescence intensity measurements and that from chromatographic analysis to evaluate poly(3-hydroxybutyrate) [P(3HB)], poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)], poly(3-hydroxybutyrateco-3-hydroxyvalerate) [P(3HB-co-3HV)] and poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate)[P(3HB-co-3HV-co-4HB)], using the same calibration curve,despite of different co-monomers that the PHA consist. Strongly supported by these experimental results, it can therefore be concluded that the developed staining method can be efficiently applied for rapid monitoring of PHA production.
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M. S. Salwa,M. N. Nur Asshifa,A. A. Amirul,Ahmad R. M. Yahya 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.6
A locally-isolated=Pseudomonas aeruginosa USM AR2 possessing the ability to produce glycolipid-type biosurfactant (rhamnolipid) was used in this research to explore fermentation technology for rhamnolipid production. Rhamnolipid concentration in 2.5 L fed-batch fermentation was improved from 0.173 to 8.06 g/L by manipulating the feeding strategy and cultivation protocol. The culture was fed with petroleum diesel and complex medium. The highest rhamnolipid concentration was achieved when the culture was initially fed with both petroleum diesel and complex medium, followed by feeding of petroleum diesel only at the end of the stationary phase. Severe foaming problem was resolved by modifying and integrating a foam recycler to the bioreactor. This successfully extended the cultivation period and increased the yield of final rhamnolipid. No antifoam agent was added as this modified bioreactor allowed cultivation to proceed even under foam generation. The viscosity measurement, surface tension activity test, and drop-collapse test were performed as an indirect measure of rhamnolipid presence A locally-isolated=Pseudomonas aeruginosa USM AR2 possessing the ability to produce glycolipid-type biosurfactant (rhamnolipid) was used in this research to explore fermentation technology for rhamnolipid production. Rhamnolipid concentration in 2.5 L fed-batch fermentation was improved from 0.173 to 8.06 g/L by manipulating the feeding strategy and cultivation protocol. The culture was fed with petroleum diesel and complex medium. The highest rhamnolipid concentration was achieved when the culture was initially fed with both petroleum diesel and complex medium, followed by feeding of petroleum diesel only at the end of the stationary phase. Severe foaming problem was resolved by modifying and integrating a foam recycler to the bioreactor. This successfully extended the cultivation period and increased the yield of final rhamnolipid. No antifoam agent was added as this modified bioreactor allowed cultivation to proceed even under foam generation. The viscosity measurement, surface tension activity test, and drop-collapse test were performed as an indirect measure of rhamnolipid presence
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Hema Ramachandran,A. A. Amirul 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.6
Discharging the unrefined glycerine, a by-productfrom biodiesel production is the simplistic solution adoptedfor its management which has led to its price reduction inthe market worldwide and created serious environmentalimpact. Therefore, we have explored the application ofunrefined glycerine pitch as direct fermentative substratein the biosynthesis of novel yellow-pigmented poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)]copolymer by Cupriavidus sp. USMAHM13 through onestagecultivation. Utilization of glycerine pitch (10 g/L)together with 1,4-butanediol (5 g/L) had resulted in thehighest achievement of 2.91 g/L of P(3HB-co-40%4HB)copolymer which was naturally dyed with the yellowpigment through the co-extraction process. Enhancementof 4HB monomer accumulation was also attained throughthe addition of ammonium acetate as nitrogen source. Itwas revealed that utilization of recovered crude glycerinefrom glycerine pitch was more preferred compared to theother recovered components. Utilization of glycerine pitchin the biosynthesis of P(3HB-co-4HB) copolymer wouldnot only contribute to the efficient waste management butalso would promote the development of cost-efficiencymicrobial fermentation.
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