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BoxBroker: A Policy-Driven Framework for Optimizing Storage Service Federation
( Rene Heinsen ),( Cindy Lopez ),( Eui-Nam Huh ) 한국인터넷정보학회 2018 KSII Transactions on Internet and Information Syst Vol.12 No.1
Storage services integration can be done for achieving high availability, improving data access performance and scalability while preventing vendor lock-in. However, multiple services environment management and interoperability have become a critical issue as a result of service architectures and communication interfaces heterogeneity. Storage federation model provides the integration of multiple heterogeneous and self-sufficient storage systems with a single control point and automated decision making about data distribution. In order to integrate diverse heterogeneous storage services into a single storage pool, we are proposing a storage service federation framework named BoxBroker. Moreover, an automated decision model based on a policy-driven data distribution algorithm and a service evaluation method is proposed enabling BoxBroker to make optimal decisions. Finally, a demonstration of our proposal capabilities is presented and discussed.
Kang, Taewook,Kim, Jae Ho,Hong, Ingie,Park, Nam Hyun,Park, Nanhyun,Heinsen, Helmut,Lee, Joo-Yong,Ravid, Rivka,Ferrer, Isidro,Yoo, Jong Shin,Kwon, Kyung-Hoon,Park, Young Mok Springer-Verlag 2014 Analytical and Bioanalytical Chemistry Vol.406 No.22
<P>Posttranslational modifications modulate protein function in cells. Global analysis of multiple posttranslational modifications can provide insight into physiology and disease, but presents formidable challenges. In the present study, we used a technique that does not require target enrichment to analyze alterations in the phosphorylation and ubiquitination of proteins from patients with Alzheimer's disease (AD). Guided by our previous findings, we applied three strategies to further our understanding of the dysregulation of posttranslationally modified proteins. We first identified phosphorylation sites by determining peptide pI shifts using OFFGEL. Second, using tandem mass spectrometry, we determined the ubiquitination status of the proteins using an assay for a trypsin digestion remnant of ubiquitination (Gly-Gly). Third, for large-scale discovery, we quantified the global differences in protein expression. Of the proteins expressed in AD tissue at levels of 2.0 or greater compared with controls, 60 were phosphorylated and 56 were ubiquitinated. Of the proteins expressed at levels of 0.5 or lower compared with controls, 81 were phosphorylated and 56 were ubiquitinated. Approximately 98?% of the phosphopeptides exhibited a pI shift. We identified 112 new phosphorylation sites (51.38?%), and 92 new ubiquitination sites (96.84?%). Taken together, our findings suggest that analysis of the alterations in posttranslationally modified proteins may contribute to understanding the pathogenesis of AD and other diseases.</P>
Molina, Mariana,Steinbach, Simone,Park, Young Mok,Yun, Su Yeong,Di Lorenzo Alho, Ana Tereza,Heinsen, Helmut,Grinberg, Lea T,Marcus, Katrin,Leite, Renata E Paraizo,May, Caroline Springer 2015 JOURNAL OF NEURAL TRANSMISSION Vol.122 No.7
<P>Brain function in normal aging and neurological diseases has long been a subject of interest. With current technology, it is possible to go beyond descriptive analyses to characterize brain cell populations at the molecular level. However, the brain comprises over 100 billion highly specialized cells, and it is a challenge to discriminate different cell groups for analyses. Isolating intact neurons is not feasible with traditional methods, such as tissue homogenization techniques. The advent of laser microdissection techniques promises to overcome previous limitations in the isolation of specific cells. Here, we provide a detailed protocol for isolating and analyzing neurons from postmortem human brain tissue samples. We describe a workflow for successfully freezing, sectioning and staining tissue for laser microdissection. This protocol was validated by mass spectrometric analysis. Isolated neurons can also be employed for western blotting or PCR. This protocol will enable further examinations of brain cell-specific molecular pathways and aid in elucidating distinct brain functions.</P>