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Jeong, Heejin,Lee, Byung-Il,Byeon, Song-Ho The Royal Society of Chemistry 2014 New journal of chemistry Vol.38 No.12
<P>A new strategy is described for the synthesis and the size control of REVO<SUB>4</SUB> (RE = rare earths) nanoparticles in aqueous solutions at room temperature. In particular, Eu-doped layered gadolinium hydroxychloride was explored as a representative precursor to react with meta-vanadate (VO<SUB>3</SUB><SUP>−</SUP>).</P> <P>Graphic Abstract</P><P>A new strategy is described for the synthesis and the size control of REVO<SUB>4</SUB> (RE = rare earth) nanoparticles in aqueous solutions at room temperature. In particular, Eu-doped layered gadolinium hydroxychloride was explored as a representative precursor to react with meta-vanadate (VO<SUB>3</SUB><SUP>−</SUP>). <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c4nj01560f'> </P>
Directional self-assembly of rare-earth hydroxocation nanosheets and paradodecatungstate anions
Jeong, Heejin,Lee, Byung-Il,Byeon, Song-Ho The Royal Society of Chemistry 2012 Dalton Transactions Vol.41 No.46
<P>Simply mixing layered rare-earth hydroxide colloidal solutions and paratungstate solution at pH > 7 led to directional self-assembly of rare-earth hydroxide nanosheets and paradodecatungstates. These hybrids exhibit efficient energy transfer from the OMCT band to rare-earth ions in the hydroxide layers as well as highly enhanced f–f transitions.</P> <P>Graphic Abstract</P><P>Simply mixing layered rare-earth hydroxide colloidal solutions and paratungstate solution at pH > 7 led to directional self-assembly of rare-earth hydroxocation nanosheets and paradodecatungstate anions. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2dt32421k'> </P>
Jeong, Heejin,Lee, Byung-Il,Byeon, Song-Ho American Chemical Society 2016 ACS APPLIED MATERIALS & INTERFACES Vol.8 No.17
<P>The excitation of the adsorbed vanadate group led to the red emission arising from the efficient energy transfer to Eu-doped layered gadolinium hydroxide (LGdH:Eu). This light-harvesting antenna effect allowed LGdH:Eu to detect selectively a vanadate in aqueous solution at different pHs. Because vanadate exists in various forms by extensive oligomerization and protonation reactions in aqueous solution depending on pH, it is important to detect a vanadate regardless of its form over a wide pH range. In particular, spacer molecules with long alkyl chains greatly facilitated access of a vanadate antenna into the interlayer surface of LGdH:Eu. The concomitant increase in adsorption capacity of LGdH:Eu achieved a strong antenna effect of vanadate on the red emission from Eu3+. When a suspension containing LGdH:Eu nanosheets (1.0 g/L) was used, the vanadate concentration down to 1 X 10(-5) M could even be visually monitored, and the detection limit based on the D-5(0) -> F-7(2) emission intensity could reach 4.5 x 10(-8) M.</P>
Heejin Jeong,Myung Jin Oh,Nari Seo,Hyun Joo An 한국당과학회 2022 한국당과학회 학술대회 Vol.2022 No.07
In recent years, liquid biopsy has been noted for its outstanding advantages over tissue biopsy including ease of sampling, effective monitoring, and longitudinal assessment of treatment dynamics. In particular, Cerebrospinal fluid(CSF), a body fluid that directly interacts with the extracellular fluid of the brain and central nervous system, is a representative body fluid used for liquid biopsy and is becoming a potential source for neurological study. Mucopolysaccharidosis is a fatal neurodegenerative lysosomal storage disease caused by lysosomal β-gal deficiency and of galactose-containing substrates accumulate in various tissues such as brain and liver. Therefore, continuous monitoring of changes in the expression of galactose substrates in the brain is important for the diagnosis and treatment of disease. However, the low protein content of the biofluid makes this difficult. Here, we developed a highly sensitive, absolute quantitation platform for N-glycans containing terminal galactose(A2G2/A2G2F) to determine mucopolysaccharidosis using liquid biopsy of mouse CSF within 2hours. N-glycans labeled with InstantPC were quantified using hydrophilic interaction chromatography(HILIC) coupled with multiple response monitoring(MRM)-MS. In particular, A2G2/A2G2F glycans are present in less than 5% even in very small amount of mouse CSF, but it is possible to quantify glycans in 300nL mouse CSF at the femtomole level. Indeed, we were able to achieve high reproducibility within 15% of CV in experimental/instrumental replicates in the concentration range of 0.1 to 500pg/μL with quantitative linearity. Our glycan quantification platform can be potentially applied for diagnosis and monitoring of patients for neurodegenerative diseases at trace amounts of CSF (about 5μL) in a much more convenient and non-invasive manner.
TRPM2 contributes to LPC-induced intracellular Ca<sup>2+</sup> influx and microglial activation
Jeong, Heejin,Kim, Yong Ho,Lee, Yunsin,Jung, Sung Jun,Oh, Seog Bae Elsevier 2017 Biochemical and biophysical research communication Vol. No.
<P><B>Abstract</B></P> <P>Microglia are the resident immune cells which become activated in some pathological conditions in central nervous system (CNS). Lysophosphatidylcholine (LPC), an endogenous inflammatory phospholipid, is implicated in immunomodulatory function of glial cells in the CNS. Although several studies uncovered that LPC induces intracellular Ca<SUP>2+</SUP> influx and morphologic change in microglia, there is still no direct evidence showing change of phosphorylation of mitogen-activated protein kinase (MAPK) p38 (p-p38), a widely used microglia activation marker, by LPC. Furthermore, the cellular mechanism of LPC-induced microglia activation remains unknown. In this study, we found that LPC induced intracellular Ca<SUP>2+</SUP> increase in primary cultured microglia, which was blocked in the presence of Gd<SUP>3+</SUP>, non-selective transient receptor potential (TRP) channel blocker. RT-PCR and whole cell patch clamp recordings revealed molecular and functional expression of TRP melastatin 2 (TRPM2) in microglia. Using western blotting, we also observed that LPC increased phosphorylation of p38 MAPK, and the increase of p-p38 expression is also reversed in TRPM2-knockout (KO) microglia. Moreover, LPC induced membrane trafficking of TRPM2 and intrathecal injection of LPC increased Iba-1 immunoreactivity in the spinal cord, which were significantly reduced in KO mice. In addition, LPC-induced intracellular Ca<SUP>2+</SUP> increase and inward currents were abolished in TRPM2-KO microglia. Taken together, our results suggest that LPC induces intracellular Ca<SUP>2+</SUP> influx and increases phosphorylation of p38 MAPK via TRPM2, which in turn activates microglia.</P> <P><B>Highlights</B></P> <P> <UL> <LI> TRPM2 channels are functionally expressed in microglia. </LI> <LI> TRPM2 channels are involved in LPC-induced p38 MAPK phosphorylation. </LI> <LI> LPC-induced [Ca<SUP>2+</SUP>]<SUB>i</SUB> increase and inward currents are dependent on TRPM2 channels. </LI> </UL> </P>