옥수수 수염의 휘발성 성분

곽재진,이재곤,장희진,김옥찬 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.4

옥수수 수염에서 purge와 trap headspace 장치를 이용하여 분리된 휘발성 성분으로부터 표준품과의 mass spectrum 및 GC 머무름 시간의 비교에 의해 44종의 휘발성 성분을 확인하였다. 확인된 성분을 기능기별로 나누어 보면 alcohol 성분들이 9종, aldehyde와 ketone의 carbonyl 화합물이 7종, terpene 관련 화합물이 14종, pyrazine계 화합물이 3종, hydrocarbon 화합물 5종 및 기타 성분이 6종으로 나타났다. Peak의 면적비(%)에 의한 양적인 면을 비교하여 2-propanol(8.08%), pentanol(1.82%), hexanol(2.86%), heptanol(1.26%), nonanol(1.28%) 등의 비점이 낮고 분자량이 작은 alcohol 화합물, alcohol의 산화 생성물인 hexanal (3.68%), heptanal(7.40%), nonanal(7.93%), decanal(2.04%) 등의 aldehyde 화합물, limonene(1.68%), α-copaene(2.20%), β-caryophyllene(1.43%), β-selinene(1.03%), α-selinene(1.03%) 등의 terpene 관련 화합물과 methyl pyrazine(0.28%), 2,5-dimethyl pyrazine(0.09%), 2,6-dimethyl pyrazine(0.41%) 등의 pyrazine 화합물 등이 주요 향기성분으로 확인되었다. Volatile components of Cornsilk(Zea mays L.) were isolated by purge and trap headspace method and were analyzed by GC and GC/MSD. A total of 44 components were identified in the cornsilk volatile components including 9 alcohols, 7 aldehydes and ketones, 14 terpenes and terpene alcohols, 3 pyrazines, 5 hydrocarbons and 6 miscellaneous components. The major components were 2-propanol(8.08%), pentanol(1.82%), hexanol(2.86%), hexanal(3.68%), heptanal(7.40%), nonanal(7.93%), decanal(2.04%), α-copaene(2.20%), limonene(1.68%), α-selinene(1.03%), β-selinene(1.03%).

Solid Phase Micro Extraction을 이용한 산초의 휘발성 성분 분석

이재곤,장희진,곽재진,김옥찬,이계호 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.2

Solid phase micro extraction(SPME)법의 최적조건 실험 및 SPME로 추출된 산초(Zanthoxylum schinifolium)의 휘발성 성분을 GC/MSD로 확인하였다. 휘발성 성분 추출에 자주 이용되는 동시증류추출(SDE)법으로 산초의 휘발성분을 분석하여 SP-ME 법에서 확인된 휘발성 성분과 조성비를 비교하였다. SPME 최적조건 시험에서 분자량이 적고 비점이 낮은 성분은 온도가 증가할수록 화이버에 흡착되는 양은 적게 나타났으며, 분자량이 크고 비점이 높은 성분은 추출시간이 증가할수록 흡착되는 양은 증가하였다. SPME 최적조건인 추출시간 30분과 50℃에서 분리된 휘발성 성분에서는 limonene(14.65%), geranyl acetate(11.07%), β-phellandrene(7.42%), 및 phellandral(3.08%) 등의 monoterpenoids 화합물과 caproic acid(11.99%), caprylic acid(8.01%), heptanoic acid(3.49%) 등의 지방산 화합물이 주요 성분으로 확인되었고, SDE법으로 추출된 휘발성 성분에서는 geranyl acetate(13.31%), limonene(12.81%) β-phellandrene(8.86%), trans-geraniol(5.22%) 및 caprylic acid(3.03%) 등의 화합물이 주요 성분으로 확인되었다. 지방산 성분들은 SPME법에서 높게 나타난 반면에 알코올 성분들은 SDE법보다 낮았다. SPME법은 적은 양의 시료로도 매우 신속하고 간단하게 전처리 할 수 있기 때문에 기존의 휘발성분 분석시 주로 사용한 SDE법에 비해 편리하며 경제적인 것으로 생각된다. Test of optimum condition of solid phase micro extraction(SPME) was performed by use of 5 volatile components in dilute aqueous solution. Volatile components of Sancho(Zanthoxylum schinifolium) were isolated by SPME method and were analyzed by GC/MSD, and compared with volatile components isolated by simultaneous distillation extraction(SDE) method. Total 31 components were identified by comparing gas chromatography retention time and mass spectral data. The major compounds were limonene, geranyl acetate, β-phellandrene, phellandral, myrcene, linalool, rose oxide, caproic acid and caprylic acid. SPME sampling procedure was found to be a good method for qualitative analysis of the volatile components.

한국산 바질(Ocimum basilicum L.)의 휘발성 성분

이재곤,안대진,곽재진,장희진,정기택,이종철 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.5

동시증류추출장치를 사용하여 분리한 Sweet계 바질(Ocimum basilicum)의 4개 품종(anise, darkopal, lettuce, sweet)의 휘발성 성분을 GC/MSD로 확인하였다 확인된 성분을 기능기별로 분류하면 alcohol 성분들이 11종, carbonyl 화합물이 6종, terpene 관련 화합물이 19종. ester 화합물이 5종으로 나타났다. 주요 화합물들은 1,8-cineole(2.86∼5.04%), camphor(1.07∼l.87%), α-Bergamotene(2.05∼5.40%), methyl chavicol(0∼25.49%), eugenol (3.55∼13.53%) 등의 carbonyl 화합물과 linalool(28.52∼36.50%), bornyl acetate(0.48∼2.79%), methyl cinnamate(0∼23.12%)등의 alcohol과 ester류, cis-ocimene(1.15∼2.02%), β -cubebene(2.77∼4.80%), cadinene(1.08∼1.70%)등의 terpene 화합물이 주요 성분으로 확인되었다. Darkopal, lettuce, sweet 바질은 linalool(30.09∼35.50%), methyl chavicol(8.09∼25.49%)이 주요 성분으로 나타났고, Anise 바질은 linalool(28.52%), methyl cinnamate(23.12%)가 주요 성분으로 확인되었으나 바질의 주요성분으로 알려져 있는 methyl chavicol은 검출되지 않았다. The essential oils of anise, darkopal, lettuce and sweet basils cultivated in Korea were extracted by simultaneous steam distillation and extraction method and were analyzed by GC/MSD. Total 41 components were identified in essential oils including 11 alcohols, 6 carbonyls, 19 hydrocarbons and 5 esters components. The major components were 1,8-cineole, linalool, α-bergamotene, β-elemene, methyl chavicol, β-cubebene, methyl cinnamate and eugenol. Darkopal, lettuce and sweet basils were rich in linalool(30. 1∼36.5%), methyl chavicol(8.1∼25.5%) and relatively poor in methyl cinnamate(0∼2.71%). Anise basil was rich in linalool(28.5%) and methyl cinnamate(23.1%). However, methyl chavicol, which was known as one of the main components of basil, was not detected.

추출방법에 따른 솔잎의 휘발성 성분 조성 비교

이재곤 ( Jae Gon Lee ),이창국 ( Chang Gook Lee ),백신 ( Shin Baek ),권영주 ( Young Ju Kwon ),장희진 ( Hee Jin Jang ),곽재진 ( Jae Jin Kwag ),이문수 ( Moon Soo Rhee ),이계호 ( Gae Ho Lee ) 한국식품영양학회 2006 韓國食品營養學會誌 Vol.19 No.2

Purge and Trap Headspace 법에 의한 솔잎 (Pinus densiflora S.)의 휘발성 성분

이재곤 ( Jae Gon Lee ),이창국 ( Chang Gook Lee ),장희진 ( Hee Jin Jang ),곽재진 ( Jae Jin Kwag ) 한국식품영양학회 2004 韓國食品營養學會誌 Vol.17 No.3

추출방법에 따른 생강의 휘발성 성분 조성 비교

이재곤,장희진,곽재진,이동욱 한국식품영양학회 2000 韓國食品營養學會誌 Vol.13 No.1

식물체의 향기성분을 분리하는데 주로 사용되는 headspace법, SPME법 및 SDE법으로 생생강(Zin-giber officinale R.)으로부터 총 61종의 휘발성 성분을 확인하였다. 확인된 성분은 alcohol류가 19종, carbonyl 화합물이 15종, terpene관련 화합물이 23종, ester 화합물이 4종으로 나타났다. Headspace법에서는 SDE법에서 분리하기 어려운 휘발성이 강한 acetaldehyde, ethyl acetate, 2,3-butandione 등과 같은 저비점 휘발성 성분들이 확인되었고, SPME법에서는 총 31종의 휘발성 성분이 확인되었다. SDE법에서는 총47개의 성분을 확인하였으며 headspace법과 SPME법에서 확인되지 않은 고비점 성분들인 elemol, zingiberenol, β-eudesmol, β-sesquiphellandrol등과 같은 sesquiterpenoids 성분들이 확인되었다. Peak의 면적비(%)에 의한 방법간의 양적인 면을 비교하여 보면 생강의 주성분인 zingiberene의함량은 SPME법에서 41.5%로 가장 많았으며, headspace법 9.4%, SDE법 8.3%로 나타났다. 생강 냄새에 가장 중요한 역할을 하는 것으로 알려진 neral과 geranial의 피크면적(%)은 SDE법에서 7.7∼10.7%로 가장 많았으며, headspace법 0.3∼1.8%, SPME법 0.9∼2.0%로 나타났다. 3가지 추출방법으로부터 확인된 성분과 그들의 조성은 차이가 있는데 이는 전처리시 추출조건 및 흡착물질이 다르기 때문인 것으로 생각된다. 따라서 천연물로부터 휘발성 성분을 확인하고자 할 때 essentialoil 중에 함유된 휘발성분 분석에는 SDE법이 좋을 것으로 판단되며, 저비점 휘발성분 분석에는 headspace법이 양호한 것으로 판단된다. The volatile components of Korean ginger were compared by using different isolation methods, head-space sampling procedure(HSSP), simultaneous distillation extraction(SDE) and solid phase micro-extraction(SPME). Sixty-one components were identified by GC-MSD in the extracts obtained from each extraction methods. However, the components identified showed a difference in their composition with the extraction methods. In the extract by HSSP, fifty-five components including a high volatile compound such as acetaldehyde, ethylacetate, 2,3-butandione were detected, and thirty-one components were identified in the extract by SPME. While, the low volatile components such as elemol, zingiberenoland β-eudesmol were detected only in the extract by SDE method. The results suggest that SDE method is the best for the analysis of low volatile components, whereas HSSP is a proper method for the anamlysis of high volatile components from natural resources.

SPME GC-MS을 이용한 솔잎의 휘발성 성분 조성 비교

이재곤 ( Jae Gon Lee ),이창국 ( Chang Gook Lee ),백신 ( Shin Back ),장희진 ( Hee Jin Jang ),곽재진 ( Jae Jin Kwag ),이계호 ( Gae Ho Lee ) 한국식품영양학회 2005 韓國食品營養學會誌 Vol.18 No.4

Comparison of Pyrolytic Components in lamina and Midrib of Flue-Cured Tobacco Leaves

Lee, Jae-Gon,Jang, Hee-Jin,Kwag, Jae-Jin,Lee, Dong-Wook The Korean Society of Tobacco Science 2000 한국연초학회지 Vol.22 No.2

This study was conducted to compare the volatile components of lamina(cutter group) and midrib of flue-cured tobacco leaves by two analytical methods, Curie-Point pyrolysis and Purge & Trap headspace technique. The pyrolysis of lamina and midrib part of tobacco leaves was performed at the temperature of $330^{\circ}C$, $650^{\circ}C$, and $920^{\circ}C$ by Curie-Point Pyrolyzer, and 33 compounds were identified in the pyrolyzates by GC/MSD. The composition of the components identified showed a quite difference between lamina and midrib. However, the amount of the pyrolyzed products from the both of lamina and midrib was increased with temperature increase except that of acetic acid, furfural, and nicotine. The content of phenolic compounds including phenol, 4-methyl phenol, and 3-methyl phenol was higher in midrib than in lamina, while that of furan compounds such as 2,3-dihydrobenzofuran, 5-hydroxymethyl furfural, was high in lamina. Interestingly, acetamide, 2-propenamide and 3-acetoxy pyridine were not defected in the pyrolyzates of lamina. By Purge & Trap headspace technique, 28 volatile components were identified in both lamina and midrib. The composition of the identified compounds and their chromatograpic patterns also showed the complete difference between the two. The content of solanone, $\beta$-damascone, $\beta$-damascenone, and megastigmatrienones, key components of tobacco aroma, was much higher in lamina than in midrib. The results indicate that lamina contains much more carbonyl compounds known to enhance the smoke taste of cigarette, whereas midrib takes nitrogenous and phenolic compounds, which are known to cause a deteriorate effect of smoke such as irritation.

Comparison of Pyrolysis Patterns of Different Tobacco Leaves by Double-Shot Pyrolysis-GC/MSD Method

Lee, Chang-Gook,Lee, Jae-Gon,Jang, Hee-Jin,Kwon, Young-Ju,Lee, Jang-Mi,Kwag, Jae-Jin,Kim, Soo-Ho,Sung, Yong-Joo,Shin, Chang-Ho,Kim, Kun-Soo,Rhee, Moon-Soo The Korean Society of Tobacco Science 2008 한국연초학회지 Vol.30 No.2

In this paper, we describe our study on the characterization of tobacco leaves by their pyrolysis patterns. Two kinds of tobacco leaves were pyrolyzed and analyzed by Double-Shot Pyrolysis-Gas Chromatography/Mass Spectroscopy (Py-GC/MS) methods. Three grades of Korean flue-cured tobacco leafsuch as B1O, AB3O, CD3L and burley tobacco leaves such as B1T, AB3T, CD3W were pyrolyzed with six discrete but stepwise heating temperature ranges, those are from 100$^{\circ}C$ to 150$^{\circ}C$, 150$^{\circ}C$ to 200$^{\circ}C$, 200$^{\circ}C$ to 250$^{\circ}C$, 250$^{\circ}C$ to 300$^{\circ}C$, 300$^{\circ}C$ to 350$^{\circ}C$ and finally from 350$^{\circ}C$ to 400$^{\circ}C$. Using the resultant 52 pyrolytic components identified in the programs as components, principal component analysis (PCA) showed statistical classification between flue-cured and burley tobacco lamina. Among six pyrolysis temperature ranges, the best discrimination was achieved at the temperature range from 250$^{\circ}C$ to 300$^{\circ}C$ and from 300$^{\circ}C$ to 350$^{\circ}C$.

A Novel Monoclonal Antibody against TRAIL Receptor 1 Induces Apoptotic Cell Death in Various Cancer Cells

Eun-Sil SUNG,Kyung-Jin PARK,Seung-Hyun LEE,Yoon-Seon JANG,Sang-Koo PARK,Yoo-Hoi PARK,Won-Jae KWAG,Myung-Hee KWON,Yong-Sung KIM 한국생물공학회 2009 한국생물공학회 학술대회 Vol.2009 No.10