Efficacy of Intracytoplasmic Sperm Injection for Leukocytospermia

강희규,--,--,--,--,--,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.1

White blood cells (WBCs) are present in most human ejaculates, but abnormally high concentration of seminal leukocytes map reflect an underlying pathological condition. The World Health Organization (WHO) has defined leukocytospermia as status of more than 10^(6) WBC/mL of semen. The purpose of this study was firstly, to compare the outcomes between conventional IVF and ICSl in leukocytospermia, and secondly, to investigate whether ICSI may be an alternation treatment for patients with leukocytospermia. Total 346 cycles of conventional IVF and ICSI candidates underwent IVF cycles at Eulji Hospital Infertility Clinic. Semen Parameters including concentration, motility, morphology of spermatozoa and concentration of leukocytes were assessed from the raw ejaculates. There was no difference in sperm concentration, motility and morphology. The rates of fertilization and good embryo development from ICSI were significantly higher than those from conventional IVF in leukocytospermia (60.4% & 32.5% respectively for ICSI group and 44.4% & 28.5%, respectively for IVF group, P<0.001). The pregnancy rate after ICSI was also higher than that from conventional IVF (34.0% vs 29.1%. P<0.05). These results indicate that the presence of seminal leukocyes (>1×10^(6) WBC/mL of semen) is adversely related with fertilization, embryo development and pregnancy rate. Therfore the measurement of seminal leukocytes in routine semen analysis appears to be of prognostic value with regard to male fertilizing potential. In conclusion. it is suggested that ICSI is an alternative choice of treatment for patients with leukocytospermia.

황사와 비황사기간의 중금속 농도분포 특성 : 2001년 황사기간에 대한 비교연구

최규훈,김기현,강창희,이진홍 濟州大學校 基礎科學硏究所 2003 基礎科學硏究 Vol.16 No.1

In this study the distribution patterns of the metallic components were analyzed both before and after the Asian Dust(AD) events at 2001 by comparing the chemical composition of metallic components in terms of various statistical methods. According to the AD/NAD concentration ratio of metallic components, the main components of crustal soils were exhibiting the values above 1.0; but opposite results were seen dominantly for hazardous metallic components. Examination of fine-to-coarse(F/C) ratios of metallic components showed higher values for major anthropogenic components including Pb(5.83), Ni(2.61), etc. Comparison of our measurement data with those obtained within and across the Korean peninsula indicated that the metallic distribution patterns of the study area can be distinguished from previous studies. The results of our analysis, when investigated in relation with air mass movement patterns, indicated evidence of the direct influence of AD events and anthropogenic processes.

기계학습기법을 통한 불임환자 데이터의 종속성 분석

정용규,강희규,박원일 凡石學術奬學財團 2002 凡石學術論文集 Vol.6 No.1

A large amount of data is obtained from patients seeking to achieve pregnancy through medical treatment. The data evolved from infertility treatment must be considered in many aspects about their characteristics and it is often difficult to find the cause of the problem due to many unexplained factors involved with infertility. The complexity of data makes it difficult for specialists to decide the correct therapy to approach. This paper presents our empirical research in Bayesian network. We constructed a Bayesian network and intestigated the utiity of the Bayesian network in identifying the relations between the factors affecting medically assisted conception. The Bayesian network classifier derived from the field data corresponds to confirms the domain knowledge that the age of female partner and the total number of embryo transferred play the key role in pregnancy. Applied to test cases for predicting target value of pregnancy, the Bayesian network shows the highest accuracy rate compared to heuristic and Naive-Bayes network classifiers.

Alteration of Spindle Formation and Chromosome Alignment in Post-Ovulatory Aging of Mouse Oocytes

Kang Hee-Gyoo,Cha Byung-Hun,Jun Jin-Hyun 한국발생생물학회 2011 발생과 생식 Vol.15 No.3

The objective of this study was to elucidate the dynamics of microtubules in post-ovulatory aging in vivo and in vitro of mouse oocytes. The fresh ovulated oocytes were obtained from oviducts of superovulated female ICR mice at 16 hours after hCG injection. The post-ovulatory aged oocytes were collected at 24 and 48 hours after hCG injection from in vivo and in vitro, respectively. Immunocytochemistry was performed on -tubulin and acetylated -tubulin. The microtubules were localized in the spindle assembly, which was barrel-shaped or slightly pointed at its poles and located peripherally in the fresh ovulated oocytes. The frequency of misaligned metaphase chromosomes were significantly increased in post-ovulatory aged oocytes after 48 hours of hCG injection. The spindle length and width of post-ovulatory aged oocytes were significantly different from those of fresh ovulated oocytes, respectively. The staining intensity of acetylated -tubulin showed stronger in post-ovulatory aged oocytes than that in the fresh ovulated oocytes. In the aged oocytes, the spindles had moved towards the center of the oocytes from their original peripheral position and elongated, compared with the fresh ovulated oocytes. Microtubule organizing centers were formed and observed in the cytoplasm of the aged oocytes. On the contrary, it was not observed in the fresh ovulated oocytes. The alteration of spindle formation and chromosomes alignment substantiates the poor development and the increase of disorders from the post-ovulatory aged oocytes. It might be important to fertilize on time in ovulated oocytes for the developmental competence of embryos with normal karyotypes.

A Study on the Decondensation and Pronucleus Formation of Sperm Nucleus in the Mouse Oocyte

Kang, Hee-Gyoo,Kim, Tai-Jeon,Bae, Hyung-Joon,Moon, Hi-Joo,Lee, Ho-Joon,Yang, Hye-Young,Kim, Moon-Kyoo 대한의생명과학회 2001 Journal of biomedical laboratory sciences Vol.7 No.4

To investigate the ability to decondense sperm head penetrated into cytoplasm of the oocytes and the relationship between this ability and the level of glutatione (GSH) in mouse oocyte at various maturing stages. The fertilizability of oocytes at various stages of maturation the decondensation of sperm nucleus and the formation of male pronucleus, were observed and the levels of GSH were measured in oocyte at same stages. Besides, the relation between fertilizability and level of GSH in oocyte cytoplasm treated with L-buthionine-S, R-sulfoxmine (L-BSO), the inbitor of biosynthesis of GSH, was determined. The decondensation of sperm head was not found in GV stage and L-BSO treated oocytes. In maturing oocytes (GVBD, MI), the decondensation was found, but the formation of male pronucleus was not. The levels of GSH in oocyte cytoplasm were measured; 2.2 pmol per oocyte in the ovulated and the matured in vitro each, 1.0 pmol in GV intact oocyte, 1.3 pmol in GVBD, and 1.5 pmol in MI phase oocyte. In L-BSO treated oocytes the levels of CSH were measured 0.08~o.09 pmol per oocyte, slightly lower than GV stage oocyte. In conclusion, GSH in oocyte is supposed to be synthesized and storaged in cytoplasm during maturation. The failure of decondensation in the cytoplasm of GV stage and L-BSO treated is suggested that GSH is an essential factor in decondensing the sperm head and that the a certain level of GSH, more than in GV oocyte cytoplasm, is required in decondensation.

Discovery of proteomic biomarker candidates for classification of depression severity

Hee-Gyoo Kang,You-Rim Lee,Sora Mun,Seungyeon Lee,Yeeun Yun,Jiyeong Lee 한국분석과학회 2023 학술대회논문집 Vol.2023 No.5

Effects of Reactive Oxygen Species on DNA Stability in Human Spermatozoa

Kang, Hee Gyoo,Kim, Tai Jeon,Bae, Hyung Joon,Moon, Hi Joo,Kim, Myo Kyung,Kim, Dong Hoon,Han, Sungwon,Lee, Ho Joon,Yang, Hye Young,Kim, Moon Kyoo 대한의생명과학회 2001 Biomedical Science Letters Vol.7 No.4

This study was designed to investigate the effects of reactive oxygen species (ROS) on DNA stability in human spermatozoa. To verify human spermatozoa were incubated with xanthine-xanthine oxidase (X 100 u M-XO 50 mIU ∼ 400 mIU), H_2O_2 (125 uM ∼ 1 mM), sodium nitroprusside (SNP 0.1 uM ∼ 100 uM) or lymphocyte. Otherwise, spermatozoa were incubated under low O_2 (5%) condition. Damage of sperm DNA was analyzed by single cell electrophoresis (Comet assay) and flow cytometry after acridine orange staining. In the presence of ROS, there was increase in DNA damage. The rate of DNA single strand breakage (9.0±1.0% ∼ 46.0±4.6%) and DNA fragmentation (7.5±1.0% ∼ 29.5±4.6%) were similar regardless of the kinds of ROS and exposure time. DNA damage in the lower O_2 condition (5%) was lower than ambient O_2 condition (20%). Taken together, it suggested that sperm DNA might be damaged by ROS. In the presence of ROS, increase in DNA damage and chromatin instability was obvious in spite of short exposure. Although present study reconfirmed that sperm incubation in the low concentration of ROS have the benefit in the induction of capacitation and AR, the increase in DNA damage by ROS and possible genetic problem should be sonsidered before the human trials.

면봉시료에서 세균의 보존을 위한 최적 보관 온도와 채취 시약의 비교

이영주 ( Yeong Ju Lee ),유희상 ( Hee Sang You ),이송희 ( Song Hee Lee ),이소립 ( So Lip Lee ),이한 ( Han Lee ),성호중 ( Ho Joong Sung ),강희규 ( Hee Gyoo Kang ),현성희 ( Sung Hee Hyun ) 대한임상검사과학회 2021 대한임상검사과학회지(KJCLS) Vol.53 No.4

면봉을 사용한 샘플링 방법은 의학, 생태학, 생명공학, 법의 학 및 오염 정도 모니터링 시스템과 같은 다양한 연구 분야에서 유용하다. 샘플링에서 채취 시약은 중요한 요소 중 하나이다. 시료를 장기간 보관해야 하는 경우에는 시료 채취 키트와 시료 보관 온도가 매우 중요하다. 이 연구의 목적은 별도의 배지 없이, 세 가지 채취 시약과 세 가지 보관 온도가 살아있는 세균의 생균수에 미치는 영향을 확인하는 것이다. 대표적인 환경 세균으로 E. coli 와 S. aureus를 선정하였다. 증류수(DW), 멸균된 인산염 완충액(PBS), Tris-EDTA (TE) 버퍼를 채취 시약으로 사용하여 샘플링한 후, 22, 4, -70°C에서 보관을 진행했다. 각 채취 시약 및 보관 온도가 시료에 미치는 영향은 RLU와 CFU로 결과로 비교하였다. -70°C 보관 온도와 TE 버퍼를 사용할 때 CFU와 RLU 값은 다른 조건에서보다 일정하게 유지되는 경향이 보였다. 따라서 이 연구는 시료가 채취 직후 -70°C에서 보관되어야 하며, 채취 시약으로 TE 버퍼와 함께 사용하는 것이 좋다는 것을 시사한다. Swabs are useful and common sampling tools in various research fields, such as medicine, ecology, biotechnology, forensic medicine, and pollutant monitoring systems. Collection reagents are one of the essential components in sampling. It is important to develop a sample collection kit and designate an appropriate storage temperature because samples need to be stored for a long time. The purpose of this study was to identify the effects of three collection reagents and three storage temperatures on the recovery of living bacteria without media. We selected Escherichia coli and Staphylococcus aureus as representative environmental bacteria. Distilled water (DW), phosphate buffered saline (PBS), and Tris-EDTA (TE) buffer were used as collection reagents and stored at 22℃, 4℃, and -70℃ after sampling. The results of using each collection reagent and storage temperature on the bacteria were compared using relative light units (RLU) and the number of colony forming units (CFU). When using -70℃ storage temperature and the TE buffer, the number of living bacteria and the RLU values remained constant. It is therefore recommended that the sample be stored at -70℃ immediately after collection and a TE buffer solution be used as the collection reagent.

Breeding of New Silkworm Strain Yeonnokjam

Kang, Pil-Don,Sung, Gyoo-Byung,Kim, Kee-Young,Lee, Hee-Sam,Kim, Mi-Ja,Ji, Sang-Duk,Hong, In-Pyo,Kweon, Hae-Yong,Park, Kwang-Young,Lee, Kwang-Gil,Sohn, Bong-Hee Korean Society of Sericultural Science 2011 International Journal of Industrial Entomology Vol.23 No.1

Yeonnokjam is a newly bred high yield special silkworm strain for spring rearing, bred from Japanese originated green cocoon spinning strain Jam 315 and Chinese originated white cocoon spinning strain Jam 316. This strain showed high hatching ratio of 96% and high healthiness of 96.7% of pupatin ratio. And its spins heavier cocoon with somewhat shorter fiber length but thicker fineness. The merits of these_strains are short larval period and labor saving, and its breeding goals are producing polish green silk and use for education.

Production of Colored Cocoons by Feeding Dye-Added Artificial Diet

Kang, Pil-Don,Kim, Mi-Ja,Jung, I-Yeon,Kim, Kee-Young,Kim, Young-Soon,Sung, Gyoo-Byung,Sohn, Bong-Hee Korean Society of Sericultural Science 2011 International Journal of Industrial Entomology Vol.22 No.1

In order to produce the colored cocoons we finalized the adequate amount of dyes throughout several trial and error experiments. The proper amount of each dye required for per 100 g of each colored cocoon turned out to be 150 mg of Rhodamine, 1560 mg of Thionin, 170 mg of Neutral red, and 200 mg of N-Blue, respectively. With this amount of dyes silkworms grew without physiological disorders. In order to produce colored cocoons, artificial diet composed mainly of mulberry leaves was fed to silkworms from the beginning of 5th instar, and subsequently fed with dye included diet from $4^{th}$ day of the 5th instar. This process resultantly produced colored silkworm body from the onset of feeding and subsequently colored cocoons and eggs. Nevertheless, the dye induced color was not inherited to next generation.