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Haribalan Perumalsamy,Jun-Ran kim,JaeYeonKim,Kum Na Ra Hwang,Young-JoonAhn 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10
An assessment was made of the toxicity of basil, Ocimum basilicum, essential oil, 11 basil oil constituents, seven structurally related compounds and another 22 previously known basil oil constituents and the control efficacy of four experimental spray formulations containing the oil (1, 2, 3 and 4% sprays) to adult American house dust mites, Dermatophagoides farinae. Results were compared with those of two conventional acaricides benzyl benzoate and N,N-diethyl-3- methylbenzamide (deet). The active principles were determined to be citral, α -terpineol and linalool. Citral (24 h LC50, 1.13 μg/cm2) and menthol (1.69 μg/cm2) were the most toxic compounds, followed by methyl eugenol (5.78 μg/cm2). The toxicity of these compounds was more toxic than that of benzylbenzoate(LC50, 8.41 μg/cm2) and deet (37.67 μg/cm2). These compounds were consistently more toxic in closed versus open containers, indicating that their mode of delivery was largely a result of vapour action. Basil applied as 3 and 4% experimental sprays provided 97 and 100% mortality against the mites respectively, whereas permethrin (cis:trans, 25:75) 2.5 g/l spray treatment resulted in 17% mortality. Reasonable mite control in indoor environments can be achieved by spray formulations containing the basil oil (3 and 4% sprays) as potential contact-action fumigants.
Haribalan Perumalsamy,Jun-Ran Kim,Murugan Kadarkarai,Young-Joon Ahn 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.05
The toxicity of materials derived from the seed of Pongamia pinnata to third instars of insecticide-susceptible Culex pipiens pallens and Aedes aegypti and wild Aedes albopictus was examined using a direct contact bioassay. Results were compared with those of the currently used insecticides fenthion and temephos. The active principles of Pongamia pinnata were identified as the karanjin (1), karanjachromene (2), pongamol (3), pongarotene (4), oleic acid (5), and palmitic acid (6) by spectroscopic analysis. Based on 24 h LC50 values, karanjin (14.61 and 16.13 mg/L) was the most toxic compound, followed by oleic acid (18.07 and 18.45 mg/L) and karanjachromene (18.74 and 20.57 mg/L). These constituents were less toxic than either fenthion (LC50, 0.0031 and 0.0048 mg/L) or temephos (0.021 and 0.050 mg/L) against Ae. aegypti and Cx. p. pallens. Low toxicity was produced by pongamol (LC50, 23.95 and 25.76 mg/L), pongarotene (25.52 and 37.61 mg/L), and palmitic acid (34.50 and 42.96 mg/L). Against A. alpopictus instars, oleic acid (LC50, 18.79 mg/L) was most toxic. Low toxicity was observed with the other five constituents (LC50, 35.26- 85.61 mg/L). P. pinnata seed-derived active principles, particularly karanjin, karanjachromene, and oleic acid, merits further study as potential mosquito larvicides for the control of mosquito populations in light of global efforts to reduce the level of highly toxic synthetic larvicides in the aquatic environment.
Haribalan Perumalsamy,Nam-Jin Kim,Young-Joon Ahn 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05
The toxicity of Asarum heterotropoides root steam distillate compounds to third instar larvae of Culex pipiens pallens, Aedes aegypti, and Ochlerotatus togoi (formerly Aedes togoi) was examined using a direct contact mortality bioassay. Results were compared with those following the treatment with fenthion and temephos. A. heterotropoides root steam distillate exhibited good larvicidal activity (21.07-27.64 ppm), based on LC50 values. Potent activity was produced by safrole (LC50, 8.22-16.10 ppm), terpinolene (11.85-15.32 ppm), -terpinene (12.64-17.11 ppm), (–)-β-pinene (12.87-18.03 ppm), (+)-limonene (13.26-24.47 ppm), 3-carene (13.83-19.19 ppm), and α-phellandrene (13.84-23.08 ppm), although the larvicidal activity of these compounds was less toxic than either fenthion (LC50, 0.023-0.029) or temephos (0.016-0.020). A. heterotropoides root steam distillate and its constituents described merit further study as potential mosquito larvicides for protection from humans and domestic animals from vector-borne diseases and nuisance caused by mosquitoes.
Repellency of Plant Essential Oils against Aedes aegypti (Culicidae: Diptera).
Haribalan Perumalsamy,Nam-Jin Kim,Jun-Ran Kim,Taek-Jong Kwak,Min-Youl Chang,Young-Joon Ahn 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05
The repellency of 104 plant essential oils to female Aedes aegypti was examined using a cage-distribution assay. Results were compared with those of the conventional mosquito repellent N,N-diethyl-m-toluamide (DEET). Coriander, oreganum, pennyroyal, lemoneucalyptus, and spearmint exhibited high repellency effect (>60 minutes at 50 mg/filter paper), followed by sage, leavander, tarragon, bergamot, and neroli oils which showed moderate effective (< 40 minutes at 50 mg/filter paper (5 cm diameter)). In the light of global efforts to reduce the level of highly toxic synthetic repellents, essential oils described merit further study as potential repellents for the control of mosquito populations.
Haribalan Perumalsamy,Bong-Ki Son,Jun-Ran Kim,Seong-Hum Yeon,Young-Joon Ahn 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05
The larvicidal activity of 104 plant essential oils and their major constituents against third instar larvae of mushroom fly Camptomyia corticalis was examined using a vapor-phase mortality bioassay. Results were compared with those of the conventional insecticides dichlorvos. Over ten plant essential oils exhibited showed more than 90% mortality. At a rate of 0.2 mg/cm3 air, pulegone, and thujone, exhibited 100% morality whereas camphor, 1,8-cineole, α-pinene, thymol and menthol showed 90% mortality at 0.6 mg/cm3 air. Global efforts to reduce the level of highly toxic synthetic insecticides in the agricultural environment justify further studies on the essential oils and their constituents described as potential insecticides for the control of the mushroom fly as fumigants with contact action.
Perumalsamy, Haribalan,Sankarapandian, Karuppasamy,Veerappan, Karpagam,Natarajan, Sathishkumar,Kandaswamy, Narendran,Thangavelu, Lakshmi,Balusamy, Sri Renukadevi Elsevier 2018 Phytomedicine Vol.46 No.-
<P>Conclusion: Our study denotes that SSBC could be very effective against AGS by inducing apoptosis through intrinsic pathway and recommended for in vivo and human trials.</P>
Haribalan Perumalsamy,Kadarkarai Murugan,Young-Joon Ahn 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05
The toxicity of materials derived from seed of Pongamia pinnata Pierre toward to third instar larvae of Aedes aegypti and Culex pipiens pallens was examined using direct contact bioassay. Results were compared with those of the currently used insecticides: fenthion and temephos. The active principles of Pongamia pinnata were identified as the karanjin (1), pongamone (2), palmitic acid (3) and karanjachromene (4), by spectroscopic analysis. Based on 24h LC50 values, karanjin (14.61 and 16.13 ppm) was the most toxic compound but less effective than fenthion (0.0031 and 0.068 ppm) and temephos (0.016 and 0.056 ppm) against Ae. aegypti and Cx p. pallens. Moderate toxicity was produced by pongamone (34.50 and 39.53 ppm), palmitic acid (36.93 and 42.96 ppm), and karanjachromene (43.05 and 48.95 ppm). P. pinnata seed derived materials, particularly karanjin, merit further study as potential mosquito larvicides for the control of mosquito populations in light of global efforts to reduce the level of highly toxic synthetic larvicides in the aquatic environment
Anti-proliferative Activity of Theanine Identified in Cicada Slough on Human Prostate Cancer
Haribalan Perumalsamy,Ha Eun Song,Ji Young Yoo,Jun-Ran Kim,Soon-Il Kim,Sang-Guei Lee,Young-Joon Ahn 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04
An assessment is made of the anti-proliferative activity of cicada slough-derived materials against 10 human cancer cell lines, including PC-3 and DU145 prostate cancer cell lines, using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Results were compared with those of the commercially available anticancer agent with broad spectrum cisplatin. The ethanol extract of Cryptotympana spp. slough was proved to have anti-proliferative activity against A549 lung, AGS stomach, PC-3 and DU145 prostate, Hela cervix, HT-29 colon, MCF-7 breast, and SK-Hep-1 liver cancer cell lines except for Hep-2 larynx and SK-OV-3 ovary cancer cell lines. The biologically active constituent was characterized as the nonprotein α-amino acid theanine [2-amino-4-(ethylcarbamoyl)butyric acid] by spectroscopic analysis, including EI-MS and NMR. Theanine was isolated from the cicada slough as a new cytotoxic principle. Fifty percent inhibition concentration (IC50) values of the constituent against PC-3 was 6.52 μg/mL, respectively. The activity of theanine (IC50,6.52μg/mL) did not differ significantly from that of the anticancer agent cisplatin (IC50,7.39μg/mL) toward PC-3. In conclusion, further studies on the cicada slough-derived materials containing theanine as potential anticancer products or a lead molecule for the prevention or eradication from human prostate cancer.
Perumalsamy, Haribalan,Kim, Jun-Ran,Kim, Soon-Il,Kwon, Hyung Wook,Ahn, Young-Joon Entomology Dept., B.P. Bishop Museum 2012 Journal of medical entomology Vol.49 No.1
<P>The toxicity of pellitorine alone or in combination with (-)-asarinin, alpha-asarone, methyleugenol, or pentadecane (1:1, 1:2, 1:3, 2:1, and 3:1 ratios) to third instars from an insecticide-susceptible KS-CP strain and -resistant DJ-CP colony of Culex pipiens pallens Coquillett was evaluated using a direct-contact mortality bioassay. The binary mixture of pellitorine and (-)-asarinin (3:1 ratio) was significantly more toxic against KS-CP larvae (0.95 mg/liter) and DJ-CP larvae (1.07 mg/liter) than either pellitorine (2.08 mg/liter for KS-CP and 2.33 mg/liter for DJ-CP) or (-)-asarinin (11.45 and 12.61 mg/liter) alone. The toxicity of the other binary mixtures (1:1, 1:2, 1:3, and 2:1 ratios) and pellitorine did not differ significantly from each other. Based on the co-toxicity coefficient (CC) and synergistic factor (SF), the three binary mixtures (1:3, 2:1, and 3:1) operated synergistically (CC, 250-390 and SF, 1.4-2.2 for KS-CP; CC, 257-279 and SF, 1.1-2.1 for DJ-CP). The binary mixtures of pellitorine and (-)-asarinin merit further study as potential larvicides for the control of insecticide-resistant mosquito populations.</P>
Larvicidal activity of Asarum sieboldii Miq against dengue vectorAedes aegypti
Haribalan Perumalsamy,Young-Joon Ahn 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10
In view of the recently increased interest in developing plant-origin insecticides are an alternative to chemical insecticides. This study was undertaken to assess the larvicidal potential of the two (H52C1 and H5C) compounds from Asarum sieboldii Miq. against dengue vector Aedes aegypti. Among the two compounds, compound I (H52C1) was most active than the compound II (H5C) against third instar larvae of Ae. aegypti. The LC50 value of the compound I was 2.03 (95% CL, 1.51-2.76) and the compound II was 10.66 (95% CL, 9.23-12.42). These findings would be useful in promoting research aiming at the development of new agents for mosquito larval control based on bioactive chemical compounds from plant origin.