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Hu Haobin,Zheng Xudong,Hu Huaisheng,Li Yan 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.5
The essential oils from the flowers, leaves, barks, roots and fruits of A. brachypus were individually extracted by hydrodistillation, and their chemical constituents were isolated and characterized by means of gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). A total of 160 different constituents accounting for 86.45-96.54% of the total essential oils were identified, and 3 constituents were unidentified (< 0.8%), and significant qualitative and quantitative differences were observed among the oils, in which the yields varied between 0.61-1.83 (mL/100 g dried materials) under the same operational conditions. The oils from flowers, leaves, barks and roots consisted mainly of monoterpenes and sesquiterpene hydrocarbons as well as oxygenated monoterpenes and sesquiterpenes, while the fruit oil consisted mainly of fatty acids and esters. The antimicrobial activities of the essential oils was evaluated against 11 microorganisms (9 for bacteria and 2 for yeast) using agar disc diffusion and broth microdilution methods. The bacteria, including gram-positive bacteria and gramnegative bacteria, were more sensitive to the oils than yeasts.
Genetic homogeneity between populations of cotton bollworm from Xinjiang, China
Dongmei Wang,Xianming Yang,Haiqiang Li,Akedan Wuwaishi,Ruifeng Ding,Haobin Li,Hongsheng Pan,Jian Liu,Yao Xu,Yan-Hui Lu 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1
We studied the population structure of cotton bollworm (CBW), Helicoverpa armigera (Hübner) (Lepidoptera:Noctuidae), in Xinjiang, the largest cotton-growing region in China, using a fragment of cytochrome c oxidasesubunit I (COI) gene. Alignments of all 192 COI sequences revealed 28 haplotypes including 23 in southernXinjiang, 5 in eastern Xinjiang and 13 in northern Xinjiang. Negative and significant values of neutrality tests forthe Tajima's D and Fu's FS parameters, combined with the high values of haplotype diversity (Hd), low values ofnucleotide diversity (π) and a high number of low frequency haplotypes indicated a recent demographic expansionof Xinjiang CBW populations. Analysis of molecular variation (AMOVA) indicated low and non-significantgenetic structure, regardless of geographical scale or crop, with most of genetic variation occurringwithin local CBW populations. Pairwise FST analyses also indicated low genetic differentiation. This demographicevent and high gene flow could be responsible for the low genetic structure currently found. CBW populations inXinjiang need to be considered as one panmictic unit in its management, especially for the design of refuges todelay the development of resistance by this migratory pest to transgenic Bt (Bacillus thuringiensis) cotton.