Non-mashed DNA Extraction and Slide Sample Preparation from a Single Spider Mite

Han-Eul Seo,Tin Moe Khaing,Jun-Yung Ahn,Jong-Ho Lee,Kyoeong-Yeoll Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.10

Due to the very small body size of spider mite, it is difficult to prepare DNA extraction simultaneously with slide samples from a single individual. Here we developed non-mashed DNA extraction method from a single spider mite to apply for molecular as well as morphological identification. Total genomic DNA was isolated from a single female adult using Genomic DNA extraction kit without the sample homogenization. DNA content of a single spider mite was 60-90 ng, which is sufficient for the PCR analysis. However, the quantity of extracted DNA and quality of the cuticle sample were dependent on the incubation time into the lysis buffer. Our results suggest that non-mashed DNA extraction method would be useful for the identification of very small mites as well as insects at the levels of DNA and morphology.

The Effect of Azadirachtin on the Development and the Control Efficacy of Bemisia tabaci

Ohnmar Lynn,Woo-Geun Song,Han-Eul Seo,Jang-Eok Kim,Kyeong-Yeoll Lee 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

Sweet potato whitefly Bemisia tabaci is a serious pest of various economically important crops. For the control of B. tabaci in an environment-friendly way, we demonstrated the effect of azadirachtin, which is an active conpound of neem oil as an botanical insecticide, on the development of B. tabaci by using an assay of single tomato leaf. Egg hatch rates were 53.6, 50.3% at 5 and 10 ppm, respectively. Adult eclosion rates were 30.0, 22.9% at these doses. We determined the whitefly control efficacies of two application methods of neem-based products by comparing between a direct spray of liquid-type into leaves and a soil treatment of pellet-type. Soil treatment of neem was greatly inhibited adult colonization by 75%. Those plants also inhibited the rates of oviposition and larval development. However, single treatment of foliar spray of neem (5-10 ppm) did not significantly inhibit the initial colonization of adult whiteflies. Furthermore, adult colonization was gradually increased regardless of neem spray.

Species-Specific Primers for the Molecular Diagnosis of Spider Mite Species of Genus Tetranychus (Acari: Tetranychidae)

Jae-Kyoung Shim,Tin Moe Khaing,Han-Eul Seo,Jun-Yung Ahn,Jong-Ho Lee,Kyoeong-Yeoll Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.10

Spider mites are one of major pests in cultivations of various ornamental plants and also important in plant quarantine service. Due to the very small body size and high similarity within the Genus the identification of species is difficult even at the microscopic observation. To identify 5 major species (Tetranychus cinnabarinus, T. urticae, T. phaselus, T. kanzawai and T. truncatus) in the Genus Tetranychus at the molecular level, we designed 4 species-specific primer sets using nucleotide sequences of the internal transcribed spacer (ITS2) region in the nuclear ribosomal repeat unit. At the PCR diagnosis of extracted genomic DNAs of 5 species using each primer set, specific primers of both T. phaselus and T. truncates were species-specific to their own species samples. However, specific primer set of T. urticae detected T. cinnabarinus as well as T. urticae. Specific primer set of T. kanzawai detected T. truncates as well as T. kanzawai, even though detection intensities were lower in non-target species.

Wilms tumor, aniridia, genitourinary anomalies, and mental retardation syndrome with deletion of chromosome 11p14.3p12

Seo, Go Hun,Kim, Yoon-Myung,Kim, Gu-Hwan,Seo, Eul-Ju,Choi, Jin Ho,Lee, Beom Hee,Yoo, Han-Wook Korean Society of Medical Genetics and Genomics 2018 대한의학유전학회지 Vol.15 No.1

WAGR (Wilms tumor, aniridia, genitourinary anomalies, and mental retardation) syndrome is a rare contiguous gene deletion syndrome caused by deleting genes including WT1 and PAX6 genes in 11p13 region, which is characterized by Wilms tumor, aniridia, genitourinary abnormalities, and intellectual disability. We report the clinical and cytogenetic characteristics of one Korean patient with WAGR syndrome. The patient shows bilateral sporadic aniridia and genital anomalies at 2 months of age. A heterozygous 14.5 Mb interstitial deletion of 11p14.3p12 region was detected by array comparative genomic hybridization. At 2 years and 10 months of age, Wilms tumor is found through regularly abdominal ultrasonography and treated by chemotherapy, radiation therapy and surgery.

Turner syndrome presented with tall stature due to overdosage of the <i>SHOX</i> gene

Seo, Go Hun,Kang, Eungu,Cho, Ja Hyang,Lee, Beom Hee,Choi, Jin-Ho,Kim, Gu-Hwan,Seo, Eul-Ju,Yoo, Han-Wook The Korean Society of Pediatric Endocrinology 2015 Apem Vol.20 No.2

<P>Turner syndrome is one of the most common chromosomal disorders. It is caused by numerical or structural abnormalities of the X chromosome and results in short stature and gonadal dysgenesis. The short stature arises from haploinsufficiency of the <I>SHOX</I> gene, whereas overdosage contributes to tall stature. This report describes the first Korean case of Turner syndrome with tall stature caused by <I>SHOX</I> overdosage. The patient presented with primary amenorrhea and hypergonadotropic hypogonadism at the age of 17 years. Estrogen replacement therapy was initiated at that time. She displayed tall stature from childhood, with normal growth velocity, and reached a final height of 190 cm (standard deviation score, 4.3) at the age of 30 years. Her karyotype was 46,X, psu idic(X)(q21.2), representing partial monosomy of Xq and partial trisomy of Xp. Analysis by multiplex ligation-dependent probe amplification detected a duplication at Xp22.3-Xp22.2, encompassing the <I>PPP2R3</I> gene near the 5'-end of the <I>SHOX</I> gene through the <I>FANCD</I> gene at Xp22.2.</P>

수지 첨부 재접합술시 출혈요법 기간의 비교 관찰

한승규,서의석,박 현,윤을식,이병일,김우경 大韓成形外科學會 2000 Archives of Plastic Surgery Vol.27 No.2

To overcome venous congestion in fingertip replantation, we have used "salvage procedure" which consists of continuous external bleeding via a stab incision on paraungal area and dripping of heparinized saline solution at the incision site to maintain external bleeding. As this method requires continuous bleeding for a certain period of time, it may be a great burden for patients, therefore it is most important to minimize the duration of bleeding for patients. Many authors have studied on the new venous channel formation of the flap. However, to our knowledge a study on the new vascular connection in fingertip replantation has not yet been carried out. Between January of 1994 to August of 1999, we performed replantations for 60 fingers in 59 patients using our salvage procedure in the Korea University Guro Hospital. Among 60 cases, 49 cases were survived including partial necrosis. We reviewed medical records of these 49 cases retrospectively. We compared and analyzed the period of external bleeding according to sex, age, level, cause of amputation, and the type of injury. The average period of the salvage procedure was 8.4 days. 6.0 days was required for the patients under the age of 10, and 7.6 days for the teens, which were the shortest salvage period groups. Based on the types of injuries, it is shorter in guillotine injury group (6.9 days) than in crush (9.0 days) or avulsion (8.7 days) groups. On the other hand, the sex and level of injury did not show much difference in the duration of the procedure.

The phenotypic heterogeneity of patients with Marfan-related disorders and their variant spectrums

Seo, Go Hun,Kim, Yoon-Myung,Kang, Eungu,Kim, Gu-Hwan,Seo, Eul-Ju,Lee, Beom Hee,Choi, Jin-Ho,Yoo, Han-Wook Williams & Wilkins Co 2018 Medicine Vol.97 No.20

<▼1><P>Supplemental Digital Content is available in the text</P></▼1><▼2><P><B>Abstract</B></P><P>Marfan syndrome (MFS) and Loeys–Dietz syndrome (LDS) are the connective tissue disorders characterized by aortic root aneurysm and/or dissection and various additional features. We evaluated the correlation of these mutations with the phenotypes and determined the clinical applicability of the revised Ghent criteria.</P><P>The mutation spectrum and phenotypic heterogeneities of the 83 and 5 Korean patients with suspected MFS and LDS were investigated as a retrospective manner. In patients with suspected MFS patients, genetic testing was conducted in half of 44 patients who met the revised Ghent criteria clinically and half of 39 patients who did not meet these criteria.</P><P>Fibrillin1 gene (<I>FBN1</I>) variants were detected in all the 22 patients (100%) who met the revised Ghent criteria and in 14 patients (77.8%) who did not meet the revised Ghent criteria (<I>P</I> <I>=</I> .0205). Patients with mutations in exons 24–32 were diagnosed at a younger age than those with mutations in other exons. Ectopia lentis was more common in patients with missense mutations than in patients with other mutations. Aortic diameter was greater in patients with missense mutations in cysteine residues than in patients with missense mutations in noncysteine residues. Five LDS patients had either <I>TGFBR1</I> or <I>TGFBR2</I> variants, of which 1 patient identified <I>TGFBR1</I> variant uncertain significance.</P><P>The revised Ghent criteria had very high clinical applicability for detecting <I>FBN1</I> variants in patients with MFS and might help in selecting patients with suspected MFS for genetic testing.</P></▼2>

Identification of 1p36 deletion syndrome in patients with facial dysmorphism and developmental delay

Seo, Go Hun,Kim, Ja Hye,Cho, Ja Hyang,Kim, Gu-Hwan,Seo, Eul-Ju,Lee, Beom Hee,Choi, Jin-Ho,Yoo, Han-Wook The Korean Pediatric Society 2016 Clinical and Experimental Pediatrics (CEP) Vol.59 No.1

Purpose: The 1p36 deletion syndrome is a microdeletion syndrome characterized by developmental delays/intellectual disability, craniofacial dysmorphism, and other congenital anomalies. To date, many cases of this syndrome have been reported worldwide. However, cases with this syndrome have not been reported in Korean populations anywhere. This study was performed to report the clinical and molecular characteristics of five Korean patients with the 1p36 deletion syndrome. Methods: The clinical characteristics of the 5 patients were reviewed. Karyotyping and multiplex ligation-dependent probe amplification (MLPA) analyses were performed for genetic diagnoses. Results: All 5 patients had typical dysmorphic features including frontal bossing, flat right parietal bone, low-set ears, straight eyebrows, down-slanting palpebral fissure, hypotelorism, flat nasal roots, midface hypoplasia, pointed chins, small lips, and variable degrees of developmental delay. Each patient had multiple and variable anomalies such as a congenital heart defect including ventricular septal defect, atrial septal defect, and patent duct arteriosus, ventriculomegaly, cryptorchism, or hearing loss. Karyotyping revealed the 1p36 deletion in only 1 patient, although it was confirmed in all 5 patients by MLPA analyses. Conclusion: All the patients had the typical features of 1p36 deletion. These hallmarks can be used to identify other patients with this condition in their early years in order to provide more appropriate care.

수정 안정수렴법을 이용한 케이블 돔의 기하학적 비선형 동적해석

서준호(Seo Jun-Ho),한상을(Han Sang-Eul),황보석(Hwang Bo Seok) 대한건축학회 2003 大韓建築學會論文集 : 構造系 Vol.19 No.6

Cable domes deform very largely because of the characteristics of flexible hybrid system and pre-tension, and include geometrical non-linearity in those structural behavior. Especially wind load is more dominant than seismic load, because cable domes are flexible structures whose bending stiffness is very small and self-weight is very light. Therefore, in this paper, the Modified Stiffly Stable Method is applied to analyze the nonlinear dynamic behavior of cable domes and compared these results with ones of the Newmark-β Method which is generally used. The Seoul Olympic Gymnastic Arena is taken as an numerical example and three kinds of models with giving each different intensity of pre-tension are selected. And dynamic nonlinear behavior of cable domes are analyzed by artificial spectrum of wind velocity wave which is similar to actual wind loads.

Clinical Features, Response to Treatment, Prognosis, and Molecular Characterization in Korean Patients with Inherited Urea Cycle Defects

Yoo, Han-Wook,Kim, Gu-Hwan,Seo, Eul-Ju The Korean Society of Inherited Metabolic Disease 2002 대한유전성대사질환학회지 Vol.2 No.1

The urea cycle, consisting of a series of six enzymatic reactions, plays key roles to prevent the accumulation of toxic nitrogenous compound and synthesize arginine de novo. Five well characterized diseases have been described, resulting from an enzymatic defect in the biosynthesis of one of the normally expressed enzyme. This presentation will focus on two representative diseases; ornithine transcarbamylase(OTC) deficiency and citrullinemia(argininosuccinate synthetase deficiency). OTC deficiency is one of the most common inborn error of urea cycle, which is inherited in X-linked manner. We identified 17 different mutations in 20 unrelated Korean patients with OTC deficiency; L9X, R26P, R26X, T44I, R92X, G100R, R141Q, G195R, M205T, H214Y, D249G, R277W, F281S, 853 del C, R320X, V323M and 10 bp del at nt. 796-805. These mutations occur at well conserved nucleotide sequences across species or CpG hot spot. The L9X and R26X lead to the disruption of leader sequences, required for directing mitochondrial localization of the OTC precursor. Their phenotypes are severe, and neonatal onset. The G100R, R277W and V323M mutations were uniquely identified in patients with late onset OTC deficiency. The other genotypes are associated with neonatal onset. Out of 20 patients with OTC deficiency, only 6 patients are alive; two were liver transplanted, and normal in growth and development at 2, 4 years after transplantation respectively. Citrullinemia is an autosomal recessive disease, caused by the mutations in the argininosuccinate synthetase(ASS) gene. We identified in 3 major mutations in 11 unrelated Korean patients with citrullinemia; G324S, $IVS6^{-2}$ A to G, and 67 bp ins at nt 1125-1126. Among these, the 67 base pair insertion mutation is novel. The allele frequency of each mutation is; G324S(45%), IVS6-2 A to G(32%), and 67 base pair insertion(14%). All patients are diagnosed at neonatal or infantile age. Interestingly, two patients presented with stroke like episode. Out of 11 patients, 5 patients died. Among 6 patients alive, one patient was successfully liver transplanted.