웨이트 트레이닝 모델에 의한 운동부하가 흰쥐 장단지근의 핵소체 형성부위 활성과 근섬유의 형태에 미치는 효과

한두석,천상우,최영자 원광대학교 생체재료·매식연구소 1995 원광생체재료·매식 Vol.4 No.1

This study was to investigate the nucleolar organizer region(NOR) associated protein by means of silver chloride staining and muscle fiber types in sections stained for SDH in the gastronecmius muscle loaded with movement by klitgaard's weight training model. Ag-NOR technique is a direct measure of the ongoing transcriptional activity of the rRNA gene or their activity. The number of Ag-NORs sites within nucleolus may reflect protein synthetic activity and proliferation. Thirty Spraque-Dawley adult female rats(190-200g) were divided into control and 3 experimental groups, which were trained for 1, 2 and 3 months. Body weight and muscle weight measured at 1, 2 and 3 months. The removed muscle was fixed by immersion, and processed for light microscopy. The results were as follows; 1. Body weight was increased, but muscle weight was decreased with the lapse of time of training. 2. After movement, the mean number of nucleolar organizer regions per nucleus was significantly increased at 2 and 3 months in gastronecmius muscle. 3. The percent of nucleolus include 4 Ag-NOR or more was increased with the lapse of time of training. 4. In muscle fiber types, type A was decreased but type C was significantly increased with the lapse of time of training.

授乳期間中 家兎 乳腺組織內 大식細胞의 動態에 關한 形態學的 觀察

韓斗錫 圓光大學校 1986 論文集 Vol.20 No.2

本 硏究는 E. coli를 注入한 家兎의 乳腺組織內에서 大? 細胞의 動態를 觀察하기 위하여 實施하였다. 乳腺組織의 病理學的 所見은 E. coli를 注入한 후 經時的으로 觀察하였으며, 大 細胞의 動態는 E. coli를 注入하여 炎症이 惹起된 乳腺內에 Trypan blue와 indian ink를 注入한 후 觀察하였다. 그 결과는 다음과 같았다. E. coli의 浮游液을 注入한 群에서는 共히 肉眼的으로 乳腺의 腫?, 充血 및 出血, 또한 限局性 壞死?와 結이 있었다. 組織學的으로는 初期에 腺胞와 乳管內에 多數의 好中球의 出現과 上皮細胞의 變成, 壞死 및 脫落], 間質內에 好中球의 浸潤, 出血 및 水腫이 觀察되었다. 時間이 經過함에 따라 ??芽細胞의 增殖과, 淋巴球, 好?球 및 大 細胞의 出現이 있었으며, 동시에 이들 細胞成分으로 構成된 限局性 壞死?가 觀察되었다. 肉芽腫性 變化가 7일째부터 認定되었다. 健康한 家兎의 授乳末技 乳腺組織의 綜合組織內에서는 授乳初期의 乳腺組織에 比하여 Trypan blue와 indian ink 粒子를 ?食한 大 細胞가 多數 觀察되었다. 그리고 炎症初期의 炎症? 周圍에서는 大 細胞를 少數를 認定할 수 있었으나 炎症이 進行됨에 따라 多數의 大 細胞가 炎症? 周圍에 出現하였다. The present study was undertaken to clarify the migration of macrophage in the mammary gland of rabbits with E. coli strains. The pathological changes were observed in mammary gland experimentally induced with E. coli strains and the migration of macrophage after injection of trypan blue or ink was examined in the mammary glands of healthy lactating rabbits and of the experimentally infected rabbits. From the results reported, the following points are ocncluded. In the bacterial suspension infused by E. coli, the affected quarters of udder showed grossly swelling, hyperemia, hemorrhage, focal necrosis ad firmnes. The microscopic findings of early stage of the mammary gland were appearance of large numbers of heterophils in the glandular lumina and ducts accompanied by degeneration, necrosis and desquamation of epithelial cells, and also infiltration of heterophils, hemorrhage and edema in the interstitial tissue, and destruction of alveoli. later, proliferation of fibroblasts, plasma cell, lymphocytes, eosinophils and macrophages appeared in the glandular tissue, and with these cells necrotic foci of glandular tissue were surrounded by highly proliferated connective tissue. in addition, glanulomatous inflammatory changes could be observed in the glandular tissue from the 7th day after infusion. Macrophages phagocytized the particles of trypan blue or indian ink were increased in number in the glandular tissue of early lactating period as compared with the later lactating period in the normal rabbits. also numerous macrophages appeared around the inflammatory foci in the course of advance of inflammation induced by bacterial suspension.

금은화 추출물의 세포독성에 관한 연구

한종현,백승화,김일광,한두석 圓光大學校 韓醫學硏究所 1993 원광한의학 Vol.3 No.1

In order to investigate the cytotoxicity of Lonicerae Flos extract, their extracts were dissolved in distilled water and methanol. All material diluted from 10^-3to 10^-7(㎎/㎖) with distilled water putted in each well of 24 multidish cultured rat fibroblast for 48hours. The cell number was calculated at 2 days and cell shape takes photographed by inverted microscope at same day. The results were as follows: 1. The rate of cell multiplication was the lowest in water extract from 10^-3 to 10^-7(㎎/㎖) concentration and the highest in ether and hexane extract at 10^-7(㎎/㎖) concentration. 2. Index of cytotoxicity was the highest with 3 score in water extract at 10^-3(㎎/㎖) concentration and the lowest with 1 score in ether, ethyl acetate or hexane extract at same concentration. 3. The degeneration of cell shape and number was the severest in water extract of 10&-4(㎎/㎖) concentration, but ether and hexane extracts in same concentration were weaker than water extract. These results indicate that the cytotoxicity of extracts by ether and hexane from same Lonicerae Flos was weaker then water extract.

수종 근관 세척액의 연쇄구균에 대한 항균효과

심재한,임미경,한두석 大韓齒科保存學會 1992 Restorative Dentistry & Endodontics Vol.17 No.1

인체 치은섬유모세포에 대한 니켈의 세포독성과 Ferulic Acid 및 Vitamin E의 독성경감효과

정윤상,한두석 원광대학교 치의학연구소 2004 圓光齒醫學 Vol.13 No.1

Nickel is major metal used in the nickel-chromium alloys of most orthodontic appliances and partial denture. But this metal is known to cause hypersensitivity, dermatitis and asthma. In addition, significant carcinogenic and mutagenic potentials has been demonstrated for compounds containing nickel. The purpose of this study was to develop the compounds repairing on the cytotoxicity of nickel in cultured human gingival fibroblasts. The cytotoxicity of nickel sulfide and nickel oxide in human gingival fibrobalsts treated with dose dependent nickel was measured by MTT assay for cell viability and XTT assay for cell adhesion activity. The cytotoxicity of ferulic acid itself also was investigated by the same cells and methods. The procedure of repairment in human gingival fibroblasts injuried by NiS_50 (nickel sulfide IC_50) and Ni0_50 (nickel oxide IC_50) was carried out to observe after treatment of ferulic acid by dose dependent manner. The 5×10⁴ cells/㎖ of human gingival fibroblasts in each well of 24 multidish were cultured. After 24 hrs, the cells were treated with solution of all groups. After the human gingival fibroblasts were cultured in same condition for 48 hrs, the colormetric methods were performed to evaluate the cytotoxicity. The light microscopic study was carried out to observe morphological changes of cultured human gingival fibroblasts. The results were as follows : 1. According to the MTT absorbance, nickel sulfide and nickel oxide were significantly decreased in 25μM and 50μM, respectively. IC_50 of nickel sulfide and nickel oxide were 138.1μM and 311.7μM, respectively. 2. According to the XlT absorbance, nickel sulfide was significantly decreased in 50 μM and nickel oxide was significantly decreased in 100μM, IC_50 of nickel sulfide and nickel oxide were 104.4μlM and 367.8μM, respectively. 3. MTT_50 and XTT_50 of ferulic acid were 2,130.3μM and 1,773.7μM, respectively. These results were determind to nontoxic by Borenfreund at., al. 4. The inhibitory effects of ferulic acid against nickel-induced cytotoxicity were significantly increased from 25μM ferulic acid, but inhibitory effects of 100μM ferulic acid was more decreased than that of 50μM ferulic acid. 5. Treatment of ferulic acid and vitamin E combination was more assumed an upward curve than that of ferulic acid only. 6. The cell numbers and cell shapes were repaired from 1μM ferulic acid. However 100μM ferulic acid showed degeneration again. These results suggest that ferulic acid and vitamin E may be decrease the cytotoxicity of nickel sulfide and nickel oxide in cultured human gingival fibroblasts.

Carbofuran이 흰쥐의 간조직과 Cytochrome P-450효소계에 미치는 영향과 Phenobarbital-Sodium의 보상효과에 관한 연구

정윤상,한두석 원광대학교 생체재료·매식연구소 1998 원광생체재료·매식 Vol.7 No.1

In order to investigate the toxicity of carbofuran and compensatory effects of phenobarbital sodium(PB) against carbofuran toxicity on hepatic tissues and cytochrome P-450 enzyme system of the rat. Sprague-Dawley male rats were administered carbofuran only, PB only or combination of carbofuran and PB. Carbofuran was severely damaged in hepatic tissues by dose-or time-dependent manner and increased the level of cytochrome P-450 enzyme system at 6hrs or 12hrs after administration. PB were not damaged in hepatic tissues, but the level of cytochrome P-450 enzyme system was increased the same as that of carbofuran. Histopathlogical changes were observed on hepatic tissues at 12hrs after carbofuran and PB combination f treatment, but were already recovered at 48hrs. PAS-positive cells were increased at 12hrs after carbofuran only administration and most hepatic cells of carbofuran and PB combination treated group showed PAS-positive reaction, but hepatic cells were normally recoverd. Also, glycogen granule were increased at 12hrs after carbofuran only administration, but glycogen granules were decreased at 12hrs. After carbofuran and PB combination treatment in best camine stain. The level of cytochrome P-450 enzyme system after carbofuran and PB combination treatment were more increased than that of carbofuran only or PB only treatment. The results indicate that PB decreased carbofuran toxicity by the elevation in the level of cytochrome P-450 enzyme system.

소엽의 가용성 부탄을 분획이 인체 구강유상피암종세포에 미치는 항암효과

박명오,한두석 원광대학교 생체재료·매식연구소 1998 원광생체재료·매식 Vol.7 No.1

This study was evaluated to the antitumor effects of the n-butanol soluble fraction of methanolic extract of perilla frutescens on human oral epitheloid carcinoma cells(KB cells). The antitumor activity of various fraction obtained from n-butanol soluble fraction of perilla frutescens was evaluated in KB cells. The antitumor of the n-butanol soluble fraction in KB cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT assay), neutral red(NR assay) and sulforhodamine B protein (SRB assay) of colormetric assay methods. The light microscopic study was carried out to observe morphological changes of cultured KB cells. These results were obtained as follows; 1. MTT and NR quantities of fraction 4 were significantly decreased by MTT and NR assay. 2. SRB quantities of fraction 4 were significantly decreased (p<0.01) by SRB assay. 3. The number of cells were decreased, cell shapes were converted to round type. 4. The fraction 4 of the n-butanol soluble fraction of perilla frutescens showed the highest antitumor activity on perilla frutescens. It has been selected as a lead fraction for further examinations.

포공령 물추출물이 뱌양 인체 구강유상피암종세포에 미치는 항암효과

이명호,한두석 원광대학교 생체재료·매식연구소 1998 원광생체재료·매식 Vol.7 No.2

In order to develop antitumor agent which indicates weak side effects and strong antitumor activity, antitumor effects of taraxaci herba water soluble extract was evaluated by MTT assay and SRB assay of colorimetric assay methods on the cultured human oral epitheloid carcinoma cells (KB cells). KB cells were cultured in RPMI 1640 media containing 10% fetal bovine serum, antibiotics and fungizone. After incubation for 24 hrs, the cells were treated with taraxaci herba water soluble extracts by dose dependent manner for 48 hrs under the same condition. The MTT and SRB quantity were measured by ELISA reader(Spectra Max 250, USA). The microscopic study was carried out to observed morphological change, Ag-NOR(argyrophylic nucleolar organizer region) number and PAS positive reacton of cultured KB cells. The results were as follows ; 1. The MTT and SRB quantity were significantly decreased in cultured KB cells treated with 10^-2 ㎎/㎖ and 10^-3 ㎎/㎖ concentration compared with control. 2. The number of Ag-NORs was significantly decreased in cultured KB cells treated with 10^-2 ㎎/㎖ concentration of taraxaci herba soluble extracts and the rate of Ag-NORs was shift left side(one Ag-NORs/nucleus was increased and five Ag-NORs/nucleus were decreased) by high concentration. 3. PAS reaction of cultured KB cells treated with 10^-2 ㎎/㎖ and 10^-3 ㎎/㎖ concentration was changed to negative. These results indicate that taraxaci herba soluble extracts may be contain antitumor compounds.

Methyl gallate가 인체 구강유상피암종세포의 증식과 부착능에 미치는 영향

박광수,한두석 원광대학교 치의학연구소 2004 圓光齒醫學 Vol.13 No.1

In order to develop antitumor agent which indicates weak side effects and strong antitumor activity, cytotoxicity and antitumor effects of methyl gallate (MG) was evaluated by MTT assay and SRB assay of colormetric assay methods on the cultured NIH3T3 fibroblasts and human oral epitheloid carcinoma cells (KB cells). NIH3T3 fibroblast were cultured in EMEM media and KB cells were cultured in RPMI 1640 media containing 10% fetal bovine serum, antibiotics and fungizone. After incubation for 24 hrs, the cells were treated with MG by dose dependent manner for 48 hrs under the same condition. The SRB quantity were measured by ELISA reader (Spectra max 250, Molecular Devices, Sunnyvale, U.S.A.). The microscopic study was carried out to observed morphological change, Ag-NORs (argyrophylic nucleolar organizer region) number and PAS positive reaction of cultured KB cells. The results were as follows; 1. The SRBm were 8701.23μM in NIH3T3 cell and 168.81μM in KB cells treated with MG. 2. The number of Ag-NORs was not significant in cultured NIH3T3 cells, but the number of Ag-NORs was significantly decreased in cultured KB cells treated with MG. 3. PAS reaction of cultured NIH3T3 cells was not changes, but PAS reaction of cultured KB cells was changed to negative reaction. 4. In cell adhesion inhibitory activity, The XTT_50 were 345.55μM in NIH3T3 cells and 49.10μM in KB cells treated with MG. 5. Morphological changes of cultured NIH3T3 cells treated with MG was weak, but KB cell treated with MG was severed.

5-Fluorouracil과 Epigallocatechin gallate가 인체 구강유상피암종세포에 미치는 항암효과에 관한 비교연구

양홍석,한두석 원광대학교 생체재료·매식연구소 1998 원광생체재료·매식 Vol.7 No.1

This study was carried out to develop antitumor agent which indicates weak side effects and strong antitumor activity. Also, the purpose of this research was to compare with the antitumor effects of 5-flurouracil(5-FU) and epigallocatechin gallate(EGCG) on cultured human oral epitheloid carcinoma cells. EGCG was reported to be the compound which indicates weak cytotoxicity against normal healthy cells such as C_3H_10T1/2 cells and inhibitory effects on the initation or promotion stage of chemical carcinogenesis is mammary gland, blood and mouse skin. Human oral epitheloid carcinoma cells (OCL 17) were cultured in RPMI-1640 media containing 10s% fetal bovine serum, antibiotic, and fungizone. The cells were treated with EGCG by dose dependent manner for 48 hrs under the same condition. The antitumor activity of 5-FU and EGCG in human oral epitheloid carcinoma cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl- 2H-tetrazolium bromide (MTT) assay. Neutral red (NR) assay and sulforhodamine B protein (SRB) assay of colorimetric methods. The light microscopic study showed morphological of the treated cells. These result were obstained as follows; 1. EGCG showed the antitumor effects high cytotoxicity against cultured human oral epitheloid carcinoma cells. 2. EGCG showed more significantly inhibitort against cultured human oral epitheloid carcinoma cells than 5-FU. 3. EGCG observed more Morphological changes than 5-FU against cultured human oral epitheloid carcinoma cells. These resullts suggest that EGCG retains a potential antitumor activity.