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RISS 인기검색어
- 검색키워드
- 저자 : Hamzah Abdulrahman Salman (검색결과 3 건)
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Characterization and expression of SabA and BabA genes in Helicobacter pylori under varying pH
Hamzah Abdulrahman Salman,Eman Mobder Nayif,Anmar Hameed Bloh 한국미생물학회 2021 미생물학회지 Vol. No.
Helicobacter pylori is a potential cause for peptic ulcers whichcan make persistent chronic infection. Helicobacter pylori produceurease at low pH to neutralize the acidic environment to colonizethe gastric mucosa. This investigation aimed to characterize H. pylori based on urease, adherence, motility, and biofilm activityand to determine the gene expression of SabA and BabA byreal-time qRT-PCR. The reference culture H. pylori ATCC49503 was employed in the current study. The characterization ofH. pylori, such as biofilm, urease, adherence, and motility assays,was determined in acidic environments with the supplement ofurea substrate. Real-time qRT-PCR was executed to find thepossible explanation for the colonization by the genes actin,SabA, and BabA. The study indicated that urea substrate isimportant in biofilm activity, adherence, motility, and urease ofH. pylori. Helicobacter pylori ATCC 49503 showed enhancedactivity at pH 2.5 only when supplemented with urea substrate. Real-time qRT-PCR confirmed the positive and significantexpression of the SabA and BabA genes in an acidic environmentand its cooperative role in biofilm and the motility of H. pylori. The results propose that urease within H. pylori is necessary toneutralize the acidic niche and colonize effectively within themucosal layers of the stomach. Additionally, the colonizationand adaptability of H. pylori in the in vitro were dependant onurease and pH. Further studies are proposed to understand thecolonization of clinical strains of H. pylori.
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Salman Hamzah Abdulrahman,Alhameedawi Alaa kamil,Muhamad Sarah Mohammed Saeed Ghofran,Taha Zahraa 한국미생물·생명공학회 2022 한국미생물·생명공학회지 Vol.50 No.1
Urinary tract infections (UTIs) are one of the most common infections in different age groups, including children. Bacteria are the main etiological agents of UTIs. The aim of the present study was to isolate, identify, and determine the antibiotic susceptibility of bacteria isolated from children with UTIs from Baghdad, Iraq. Three hundred and two urine samples were collected from children aged 6 months to 12 years. The samples were cultured on blood agar and MacConkey agar. The selected colonies were subjected to biochemical tests and antibiotic susceptibility analysis using the Vitek® 2 Compact automated microbial identification system. In this sample, 299 bacteria were identified, of which, 267 were gram-negative bacteria, and 32 were gram-positive bacteria. Escherichia coli (56%) was the most commonly isolated gram-negative bacteria, followed by Pseudomonas aeruginosa (14%), Enterobacter spp. (10.48%), Klebsiella pneumoniae (9.36%), Proteus spp. (7.8%), Acinetobacter baumannii (1.5%), and Morganella morganii (0.37%). Enterococcus faecalis (62.5%) was the most commonly detected gram-positive bacteria, followed by Staphylococcus aureus (37.5%). E. coli and P. aeruginosa were the most antibiotic-resistant bacteria. Among the tested antibiotics, meropenem showed 100% sensitivity, followed by imipenem (97.4%), amikacin (91.8%), and tobramycin (83.5%). In contrast, the high frequencies of resistance were observed with cefixime (93.2%), cefotaxime (78.7%), and ceftriaxone/cefotaxime (71.2%). In conclusion, carbapenems and aminoglycosides are highly recommended for the empirical treatment of UTIs, while, Quinolones, penicillins, and cephalosporins are not suggested. Frequent antibiotics susceptibility testing are warranted to determine the resistance pattern of UTI bacteria.
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( Anwer Jaber Faisal ),( Hamzah Abdulrahman Salman ) 한국미생물 · 생명공학회 2021 한국미생물·생명공학회지 Vol.49 No.4
Infertility is a key issue affecting mood and behavior in men. Microorganisms are one of the primary etiological agents that may be associated with infertility. The objective of the present study was to identify bacterial causative agents from the semen of infertile subjects and determine the effect of bacterial infection on sperm quality, as well as determine the susceptibility of these bacteria to drugs. Forty semen samples from 30 infertile patients and 10 fertile individuals were collected. The pH, volume, motility, and concentration of semen were analyzed. The samples were processed and identified by biochemical testing using API identification kits. The antibiotic susceptibility pattern was determined using the disc diffusion method. Abnormal sperm quality was observed. The mean age of the individual and their sperm morphology, concentration, progressive motility, pH level, and pus cell content were 31.9 years, 2.7%, 10.4 million/ml, 27.3%, 8.3, and 5.7, respectively. Among the tested samples, oligoasthenozoospermia was found to show the highest occurrence, at 27/30 samples, followed by teratozoospermia, at 25/30 samples, and asthenozoospermia, at 22/30 samples. Of the tested infertile patients' sperm, 19, 6, and 5 isolates were identified as Escherichia coli, Klebsiella pneumonia, and Staphylococcus epidermidis, respectively. The results also revealed multi-drug resistance in the bacteria. Compared to that shown by the other tested antibiotics, amikacin showed higher activity against all isolated bacteria. However, the bacteria exhibited maximum resistance against gentamicin, cefotaxime, levofloxacin, and ampicillin. In conclusion, leukocytospermia and bacterial infections are possibly responsible for sperm abnormalities. Multi-drug resistant bacteria were detected. Gentamicin, cefotaxime, levofloxacin and ampicillin were shown the highest resistance, while amikacin was the most effective antimicrobial agent against the isolated bacteria.
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