http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Hai-Yan Gan (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
Morphology and transcriptome differences between the haploid and diploid drones of Apis cerana
Wei-Yu Yan,Hai-Yan Gan,Shu-Yun Li,Jing-Hua Hu,ZilongWang,Xiaobo Wu,Zhi Jiang Zeng 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.4
In general, drone honey bees are haploid and develop from unfertilized eggs. However, a diploid drone can arise in an inbred colony. In this study, the morphological characteristics and gene expression profile of the haploid and diploid drones of Apis cerana were analyzed to reveal the differences between them. The ploidy level of the droneswas identified by FlowCytometry (FCM). The characters of the forewings,wetweight of reproductive organs and of newly emerged drones, were investigated. Then, a high throughput transcriptomic analysis was performed using RNA-seq in diploid and haploid drones. The results showed that the wet weight and reproductive organs of diploid droneswere significantly lighter than those of haploid drones. About 201 million high-quality reads were generated from RNA-seq, and 75.99–78.12% of the data weremapped to Apis cerana genome. 360 genes were differentially expressed between diploid and haploid drone, with 152 up-regulated and 208 downregulated in the diploid drones. Functional analysis identified that these genes were significantly enriched in 28 pathways. Comparative transcriptomic analysis detected several differentially expressed genes, which lay a foundation for future studies on molecular mechanisms underlying biology difference in drones in Apis cerana.
-
Transcriptome comparison between newly emerged and sexually matured bees of Apis mellifera
Xiaobo Wu,ZilongWang,Hai-Yan Gan,Shu-Yun Li,Zhi Jiang Zeng 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3
In order to understand the transcriptome characteristics of queens and drones of honeybee Apis mellifera, the transcriptome differences between newly emerged stage and sexually matured stage of queens and drones of A. mellifera L. were compared using high-throughput RNA-Seq. In drones, a total of 1618 DEGs were detected between the two stages. Out of these, 782 geneswere up-regulated and 836 geneswere down-regulated in sexually matured drones compared to newly emerged drones. In queens, the DEGs between the two stages were 1340, with 667 up-regulated and 673 down-regulated genes in matured queens compared to newly emerged queens. 411 genes showed the same expression trend in drones and queens during sexual maturing, with 233 (56.69%) up-regulated genes and 178 (43.31%) down-regulated at newly emerged stage. We found that genes encoding cuticular proteins (CP), cytochrome P450 (CYP), odorant binding proteins (OBP) and odorant receptor (OR), which are related to developments of bones, reproductive system and olfaction system, were differentially expressed between the sexually matured bees and the newly emerged bees. The results indicated that the expression levels of a large number of genes changed during sexual maturing of A. mellifera L. bees, which give us an insight into the characteristics of the gene expression during sexual maturing of adult queens and drones.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
