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- 저자 : Habibagahi, Mojtaba (검색결과 4 건)
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Tumour Suppressive Effects of WEE1 Gene Silencing in Breast Cancer Cells
Ghiasi, Naghmeh,Habibagahi, Mojtaba,Rosli, Rozita,Ghaderi, Abbas,Yusoff, Khatijah,Hosseini, Ahmad,Abdullah, Syahrilnizam,Jaberipour, Mansooreh Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11
Background: WEE1 is a G2/M checkpoint regulator protein. Various studies have indicated that WEE1 could be a good target for cancer therapy. The main aim of this study was to asssess the tumor suppressive potential of WEE1 silencing in two different breast cancer cell lines, MCF7 which carries the wild-type p53 and MDA-MB468 which contains a mutant type. Materials and Methods: After WEE1 knockdown with specific shRNAs downstream effects on cell viability and cell cycle progression were determined using MTT and flow cytometry analyses, respectively. Real-time PCR and Western blotting were conducted to assess the effect of WEE1 inhibition on the expression of apoptotic (p53) and anti-apoptotic (Bcl2) factors and also a growth marker (VEGF). Results: The results showed that WEE1 inhibition could cause a significant decrease in the viability of both MCF7 and MDA-MB-468 breast cancer cell lines by more than 50%. Interestingly, DNA content assays showed a significant increase in apoptotic cells following WEE1 silencing. WEE1 inhibition also induced upregulation of the apoptotic marker, p53, in breast cancer cells. A significant decrease in the expression of VEGF and Bcl-2 was observed following WEE1 inhibition in both cell lines. Conclusions: In concordance with previous studies, our data showed that WEE1 inhibition could induce G2 arrest abrogation and consequent cell death in breast cancer cells. Moreover, in this study, the observed interactions between the pro- and anti-apoptotic proteins and decrease in the angiogenesis marker expression confirm the susceptibility to apoptosis and validate the tumor suppressive effect of WEE1 inhibition in breast cancer cells. Interestingly, the levels of the sensitivity to WEE1 silencing in breast cancer cells, MCF7 and MDA-MB468, seem to be in concordance with the level of p53 expression.
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On The Stiffness Prediction of GFRP Pipes Subjected to Transverse Loading
Roham Rafiee,Mohammad Reza Habibagahi 대한토목학회 2018 KSCE Journal of Civil Engineering Vol.22 No.11
The main objective of this study is to predict the stiffness of GFRP pipes subjected to compressive transverse loading. An experimental study is performed to measure the stiffness of a composite pipe with a core layer of sand/resin composites. Then, a simple analytical modeling constructed on the basis of solid mechanics is used to estimate the stiffness of the investigated pipe as the back-of-envelope technique widely used by industrial sectors. The simulation of stiffness test is conducted using finite element modeling wherein both large deformation and inelastic behavior of material is taken into account as the sources of nonlinearity. The results reveal that a very good estimation with high level of accuracy can be reached by proper selection of the element and performing nonlinear analysis.
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Utilizing Fiber Reinforced Concrete as the Concrete Face of Rockfill Dams
Payam Salajegheh,Ghassem Habibagahi,S. Mohammad Sadegh Sahraeian 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.3
Construction of concrete faced rockfill dams (CFRD) includes pouring rock materials as the main body of the dam and compacting the rockfill to an acceptable compaction level. Then, a reinforced concrete slab is constructed on the upstream surface of dam to transfer water pressure to the rockfill materials. Considering the high permeability of rockfill materials, the concrete slab serves as a sealing element on the upstream side. Indeed, the required characteristics of concrete face are adequate flexibility in case of long-term settlement of dam, durability and its sufficiency in waterproofing the dam. In this study, to improve the quality of concrete face, increase its flexibility, optimize the construction cost and enhance the bearing capacity of concrete in tension, developed by bending moment in the face slab, three types of prevalent fibers (polypropylene, MEXO200 and steel) are utilized. The performance of each fiber is compared with the conventional reinforced concrete specimens by conducting some bending tests. Furthermore, carrying out numerical simulation, the efficacy of each fiber on performance of face slab is investigated. Based on the results, the fibers, especially the steel fibers, are suitable to substitute rebars of the conventional reinforced concrete employed as the concrete face of CFRDs. The steel fiber reinforced concrete has adequate capacity and ductility to mitigate the expected deformations from reservoir loading on the upstream face of CFRDs.
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Absence of Correlation between Changes in the Number of Endothelial Progenitor Cell Subsets
Armin Attar,Kamran Aghasadeghi,Mohammad Ebrahim Parsanezhad,Bahia Namavar Jahromi,Mojtaba Habibagahi 대한심장학회 2015 Korean Circulation Journal Vol.45 No.4
Background and Objectives: Previously, various methodologies were used to enumerate the endothelial progenitor cells (EPCs). We now know that these methodologies enumerate at least three different EPC subsets: circulating angiogenic cells (CACs), colony-forming unit endothelial cells (CFU-ECs), and endothelial colony-forming cells (ECFCs). It is not clear whether there is a correlation between changes in the number of these subsets. The aim of the current study is to find an answer to this question. Materials and Methods: The number of all EPC subsets was quantified in the peripheral blood of nine pregnant women in their first and third trimesters of pregnancy. We enumerated 14 cell populations by quantitative flow-cytometry using various combinations of the markers, CD34, CD133, CD309, and CD45, to cover most of the reported phenotypes of CACs and ECFCs. Culturing technique was used to enumerate the CFU-ECs. Changes in the number of cells were calculated by subtracting the number of cells in the first trimester peripheral blood from the number of cells in the third trimester peripheral blood, and correlations between these changes were analyzed. Results: The number of CFU-ECs did not correlate with the number of ECFCs and CACs. Also, CACs and ECFCs showed independent behaviors. However, the number of CACs showed a strong correlation with the number of CD133+CD309+ cells (p=0.001) and a moderate correlation with the number of CD34+CD309+ cells (p=0.042). Also, the number of ECFCs was correlated with the number of CD309+CD45- cells (p=0.029) and CD34+CD45- cells (p=0.03). Conclusion: Our study showed that the three commonly used methods for quantifying EPC subsets represent different cells with independent behaviors. Also, any study that measured the number of EPCs using the flow cytometry method with a marker combination that lacks CD309 may be inaccurate.
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