http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
金益秀,李基淑 全北大學校 生物學硏究所 1982 生物學硏究年報 Vol.3 No.-
This Study is to survey four species of Genus Microphysogobio in Korea : M.koreensis, M.yaluensis, M.tungtingensis and M.longidorsalis, and present the key to four species. To fulfil this aim, specimens were collected from major streams of South Korea from May, 1972 to September, 1981. Microphysogobio koreensis and M.longidorsalis in Korea are clearly distingushed from M.brevirostris and M.tungtingensis of China in several morphometric and meristic characters, but Microphysogobio sp.Uchida is idendified as M.tungtingensis in China.
Cordycepin이 생쥐의 난구세포 분산과 난자의 성숙에 미치는 영향
이기숙,Lee, Gy-Soog 대한생식의학회 1986 Clinical and Experimental Reproductive Medicine Vol.13 No.2
These experiments were conducted to know whether RNA syntheis is involved in the cumulus expansion and oocyte maturation of mouse cumulus-oocyte complexes in vitro. Mouse cumulus-oocyte complexes(COC's) were cultured in the presence of cordycepin, an inhibitior of RNA synthesis and its effect on the cumulus expansion and oocyte maturation were examined. The results were as follows. 1. Continuous presence of cordycepin in the medium(200${\mu}g/ml$) inhibited the HCG-induced cumulus cell expansion of mouse complexes. This inhibition was reversible. 2. When the COC'S were preincubated with different concentration of cordycepin plus HCG for 3 hours and then transferred to the plain medium, the HCG induced cumulus expansion was suppressed at $100{\mu}g/ml$ of cordycepin. 3. When the COC'S were cultured with cordycepin after HCG stimulation(3hrs), the cumulus expansion were not suppressed by cordycepin. 4. Oocyte meiotic maturation did not appear to be affected by cordycepin. The data presented here imply that RNA synthesis is involved in the cumulus expansion process and that mouse oocytes are more resistant to RNA synthesis inhibitor than cumulus cells.
인간 난자의 체외수정을 위한 정도관리로서 생쥐 착상전 배아의 배양에 관한 연구
이기숙,박종덕,이춘근,김종덕,Lee, Gy-Soog,Park, Jong-Duk,Lee, Choon-Khoon,Kim, Jong-Duk 대한생식의학회 1989 Clinical and Experimental Reproductive Medicine Vol.16 No.2
The development of 2-cell mouse embryos to the blastocyst stage in vitro has been used as quality control for the culture media and supplements employed for human in vitro fertilization and embryo transfer(IVF-ET). 2-cell mouse embryos were cultured to the blastocyst stage in SECM, Medium 199-Earle's, Ham's F-10 I , Ham's F-10 II , Hoppe & Pitts, MEM and $HT_6$. The protein supplements contained in media were bovine serum albumine, fetal bovine serum and human fetal cord serum. The results were as follows; 1. The successful development was 81.3% in Medium 199-Earle’s, 91.9% in Ham’s F-10 I and 97.1% in $HT_6$. 2. 2-cell mouse embryos developed properly in all supplements but the best development was particularly noted in $HT_6$ media when HFCS was supplied as protein supplement.
이기숙,김종덕,권혁방,Lee, Gy-Soog,Kim, Jong-Duk,Kwon, Hyuk-Bang 대한생식의학회 1990 Clinical and Experimental Reproductive Medicine Vol.17 No.2
These experiments were performed to know ultrastructural changes of the cumulus expansion in virot. SEM:In expanded oocyte-cumulus complex, the cell surface are characterized by the presence of many evaginations:they are relatively short and round shape. The mucous extracellular material were deposited between cumulus cells. TEM:In compact cumulus cells, golgi apparatus and rough endoplasmic reticulum developed. In expanded cumulus cells, rough endoplasmic reticulum decreased and the smooth endoplasmic reticulum increased. Also, there were numbers of mitochondria. Extracellular mucous material which is presumed to be hyaluronic acid appears when cumulus cell were expanded. In expanded cumulus cell, numbers of smooth endoplasmic reticulum help cumulus cell to develop in steroidogenic cell.