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Effective transduction of osteogenic sarcoma cells by a baculovirus vector
Song, Sun U.,Shin, Seok-Hwan,Kim, Soon-Ki,Choi, Gwang-Seong,Kim, Woo-Chul,Lee, Moon-Hee,Kim, Sei-Joong,Kim, In-Ho,Choi, Mi-Sook,Hong, Young-Jin,Lee, Kwan-Hee Microbiology Society 2003 The Journal of general virology Vol.84 No.3
<P>Efficient gene delivery of a baculovirus-derived vector (BV-p53-lacZ) to a human osteogenic sarcoma cell line, Saos-2, was serendipitously found while evaluating the vector for gene delivery to human p53-null tumour cells in a previous study. Therefore, we investigated other human, rat and mouse osteogenic sarcoma and other types of tumour cell lines for transduction efficiency via baculovirus vectors containing a lacZ reporter gene under the control of either a cytomegalovirus or Rous sarcoma virus promoter. The expression of beta-galactosidase protein, assessed by X-Gal staining and beta-galactosidase ELISA, demonstrated an extremely high level of transduction efficiency in some osteogenic sarcoma cell lines, such as U-2OS, Saos-2 and Saos-LM2. These human osteogenic sarcoma cell lines showed levels of beta-galactosidase expression 5-40 times greater than HepG2 cells, which were previously thought to be the mammalian cells most susceptible to baculovirus-mediated gene delivery. The level of acetylated histone proteins in these tumour lines did not correlate well with the high level of reporter gene expression. These results strongly suggest that some osteogenic sarcoma cells are highly susceptible to baculovirus-mediated gene delivery and that a baculovirus-derived vector is an efficient gene delivery vehicle into human osteogenic sarcoma cells.</P>
김소양 ( Kim So Yang ),김지훈 ( Kim Ji Hun ),김창환 ( Kim Chang Hwan ),남순우 ( Nam Sun U ),김윤정 ( Kim Yun Jeong ),김진일 ( Kim Jin Il ),박수헌 ( Park Su Heon ),한준열 ( Han Jun Yeol ),김재광 ( Kim Jae Gwang ),정규원 ( Jeong Gyu 대한소화기학회 2003 대한소화기학회지 Vol.42 No.2
Pheochromocytoma, a catecholamine-producing tumor of the chromaffin tissue, may present with various features. Herein, we report case of 66-year-old woman with pheochromocytoma accompanying hyperamylasemia and acute abdomen. She was admitted to another hospital due to myocardial infarction 5 months ago. At that time, pheochromocytoma was suggested on the basis of hormonal studies, but she refused surgical resection. When she came to our hospital, serum amylase level was 703 U/L. Subsequent studies revealed pancreatic type isoenzyme, and elevated lipase level. After normalization of serum amylase level, she undertook laparoscopic adrenalectomy. On pathologic examination, pheochromocytoma was confirmed. There are several cases of pheochromocytoma with hyperamylasemia. In general, the source of hyperamylasemia was thought to be pulmonary endothelial cells under ischemic damage caused by potent vasoconstrictive action of circulating catecholamines. In our case, analysis of isoenzymes and serum lipase level suggest that hyperamylasemia can originate from the pancreas. Thus, pancreatitis also should be considered when serum amylase level is elevated in pheochromocytoma. (Korean J Gastroenterol 2003;42:172-175)
치어의 먹이선호도 및 포식이 낙동강 동물플랑크톤군집에 미치는 영향
장광현 ( Jang Gwang Hyeon ),김현우 ( Kim Hyeon U ),라긍환 ( La Geung Hwan ),정광석 ( Jeong Gwang Seog ),주기재 ( Ju Gi Jae ) 한국하천호수학회 2004 생태와 환경 Vol.37 No.1
In the present study, pre preference of juvennile fishes was examined using as experimental approach. Zooplankton composition, as a prey of thr fish, was evaluated by taking into account the species as well as body size of juveniles in the aquqrium. The redation of juvenile fishes is known to be an important factor in changes of zooplankton communities. in some previous studies at the regulated Nakdong River, the collapse of large cladcoerans and an increase in the rotifer population by selective predation during spring and summer were observed. This study focused on the predation of juvenile fisheds such as Hyporhamphus sajori, Rhinogobius brunneus, and Opsariichtys uncirostris amurensis on zooplankton community structure in mesocosm scale experiments. These fishes selected the cladoceran Monia micrura with highest individual preference value(Manly.Chesson index) among zooplankton prey in the experimental aquarium. Whrn the size-selective prey prefernces of the juvenile fish were compared, both small (body size<2㎝)and large(body size>2㎝) juveniles of O. uncirostris positively selected M. micrura. in the outdoor experimental tanks, juvenile fishes consuumed the cladoceran M. micrura, resulting in an igh abundance of the rotifer, Polyarthra spp. The results suggest that juvenile fish predation may play an important role in regulating the zooplankton community structure by reducing the cladoceran density and increase of rotifers in the Nakdong River during spring and summer.
강-저수지 복합형 시스템내 봄 동물플랑크톤의 역동성 (낙동강, 한국): 식물플랑크톤 생체량 조절자로서의 역할
김현우 ( Kim Hyeon U ),장광현 ( Jang Gwang Hyeon ),정광석 ( Jeong Gwang Seog ),주기재 ( Ju Gi Jae ) 한국하천호수학회 2003 생태와 환경 Vol.36 No.4
During a three-year study (2000-2002), dramatic changes in the phytoplankton biomass and high transparency were repeatedly observed during mid-spring in the lower part of the Nakdong River. Rotifers(Branchonus, Keratella, Polyarthra), sharply increased (to near 20 days) and water temperature increased from 10℃ to > 20℃ toward the end of springm small cladocerans noticeably increased. Once phytoplankton biomass passed their peak stage in the min-spring, a short period (one or two weeks) of relatively low phytoplankton biomass and high Secchi transparencies occurred. Grazing by the zooplankton was highest in spring, thus, it seems that high grazing activities of zooplankton grazing regulated phytoplankton dynamics in the river. The results indicate that the role of zooplankton grazing in controlling the phytoplankton biomass becomes more important during the spring when river water is relatively stagnant.