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ON STRONG C-INTEGRAL OF BANACH-VALUED FUNCTIONS
Da fang Zhao,Guo ju Ye 충청수학회 2007 충청수학회지 Vol.20 No.1
In this paper, we de¯ne and study the Banach-valued C-integral and strong C-integral, We prove that the C-integral and the strong C-integral are equivalent if and only if the Banach space is ¯nite dimensional. We also study the primitive of the strong C-integral in terms of the C-variational measures.
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C-DUNFORD AND C-PETTIS INTEGRALS
Chao Yu,Da fang Zhao,Guo ju Ye 충청수학회 2008 충청수학회지 Vol.21 No.4
In this paper, we give some extensions of Dunford inte-gral and Pettis integral, C-Dunford integral and C-Pettis integral. We also discuss the relation among the C-Dunford integral, C-Pettis integral and C-integral.
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( Sheng Nan Li ),( Dao Sen Guo ),( Bo Guang Zhao ),( Jian Ling Ye ),( Jie Tian ),( Wen Qing Ren ),( Yun Wei Ju ),( Peng Cui ),( Rong Gui Li ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.8
A cDNA encoding a cysteine protease inhibitor (CPI) was isolated from the cDNA library of clamworm Perinereis aibuhitensis Grube. The deduced amino acid sequence analysis showed that the protein had 51%, 48%, and 48% identity with Zgc: 153129 from Danio rerio, cystatin B from Theromyzon tessulatum, and the ChainA, stefin B tetramer from Homo sapiens, respectively. The gene was cloned into the intracellular expression vector pET-15b and expressed in Escherichia coli. The recombinant CPI (PA-CPI) was purified by affinity chromatography on Nicharged resin and ion-exchange chromatography on DEAE-Sepharose FF. The relative molecular mass of PACPI was 16 kDa as deduced by SDS-PAGE. Activity analysis showed that the recombinant protein could inhibit the proteolytic activity of papain. A constitutive and secretive expression vector was also constructed, and the cDNA encoding CPI was subcloned into the vector for extracellular expression. Western blotting analysis results showed that the PA-CPI was secreted into the medium. Bioassay demonstrated that E. coli DH5α harboring pUC18ompAcat-CPI showed a significant difference in mortality to the Asian longhorned beetle Anoplophora glabripennis compared with untransformed E. coli DH5α and control.
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Kim, So-Eun,Lee, Chan-Ju,Ji, Chang Yoon,Kim, Ho Soo,Park, Sul-U,Lim, Ye-Hoon,Park, Woo Sung,Ahn, Mi-Jeong,Bian, Xiaofeng,Xie, Yizhi,Guo, Xiaodong,Kwak, Sang-Soo Elsevier 2019 Vol. No.
<P><B>Abstract</B></P> <P>Oxidative stress caused by reactive oxygen species (ROS) under various environmental stresses significantly reduces plant productivity. Tocopherols (collectively known as vitamin E) are a group of lipophilic antioxidants that protect cellular components against oxidative stress. Previously, we isolated five tocopherol biosynthesis genes from sweetpotato (<I>Ipomoea batatas</I> [L.] Lam) plants, including tocopherol cyclase (<I>IbTC</I>). In this study, we generated transgenic sweetpotato plants overexpressing <I>IbTC</I> under the control of cauliflower mosaic virus (CaMV) <I>35S</I> promoter (referred to as TC plants) via <I>Agrobacterium</I>-mediated transformation to understand the function of <I>IbTC</I> in sweetpotato. Three transgenic lines (TC2, TC9, and TC11) with high transcript levels of <I>IbTC</I> were selected for further characterization. High performance liquid chromatography (HPLC) analysis revealed that α-tocopherol was the most predominant form of tocopherol in sweetpotato tissues. The content of α-tocopherol was 1.6–3.3-fold higher in TC leaves than in non-transgenic (NT) leaves. No significant difference was observed in the tocopherol content of storage roots between TC and NT plants. Additionally, compared with NT plants, TC plants showed enhanced tolerance to multiple environmental stresses, including salt, drought, and oxidative stresses, and showed consistently higher levels of photosystem II activity and chlorophyll content, indicating abiotic stress tolerance. These results suggest <I>IbTC</I> as a strong candidate gene for the development of sweetpotato cultivars with increased α-tocopherol levels and enhanced abiotic stress tolerance.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>IbTC</I> overexpressing transgenic sweetpotato (TC plants) were generated via <I>agrobacterium</I>-mediated transformation. </LI> <LI> α-tocopherol contents in leaves were increased in TC plants compared with NT plants. </LI> <LI> TC plants showed enhanced tolerance to various environmental stresses including salt, drought and oxidative stresses. </LI> </UL> </P>
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Piao, Hai-long,Xuan, Yuan-hu,Park, Su-Hyun,Je, Byoung-Il,Park, Soon-Ju,Park, Sung-Han,Kim, Chul-Min,Huang, Jin,Wang, Guo Kui,Kim, Min-Jung,Kang, Sang-Mo,Lee, In-Jung,Kwon, Taek-Ryoun,Kim, Yong-Hwan,Ye Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.30 No.1
Calcineurin B-like protein-interacting protein kinases (CIPKs) are a group of typical Ser/Thr protein kinases that mediate calcium signals. Extensive studies using Arabidopsis plants have demonstrated that many calcium signatures that activate CIPKs originate from abiotic stresses. However, there are few reports on the functional demonstration of CIPKs in other plants, especially in grasses. In this study, we used a loss-of-function mutation to characterize the function of the rice CIPK gene OsCIPK31. Exposure to high concentrations of NaCl or mannitol effected a rapid and transient enhancement of OsCIPK31 expression. These findings were observed only in the light. However, longer exposure to most stresses resulted in downregulation of OsCIPK31 expression in both the presence and absence of light. To determine the physiological roles of OsCIPK31 in rice plants, the sensitivity of oscipk31::Ds, which is a transposon Ds insertion mutant, to abiotic stresses was examined during germination and seedling stages. oscipk31::Ds mutants exhibited hypersensitive phenotypes to ABA, salt, mannitol, and glucose. Compared with wild-type rice plants, mutants exhibited retarded germination and slow seedling growth. In addition, oscipk31::Ds seedlings exhibited enhanced expression of several stress-responsive genes after exposure to these abiotic stresses. However, the expression of ABA metabolic genes and the endogenous levels of ABA were not altered significantly in the oscipk31::Ds mutant. This study demonstrated that rice plants use OsCIPK31 to modulate responses to abiotic stresses during the seed germination and seedling stages and to modulate the expression of stress-responsive genes.
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