http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Paraquat의 殘留毒性에 미치는 완충액의 種類 및 光照射의 影響
金英美,申應男,李錦美,全相愛 효성여자대학교 가정대학 학도호국단 1984 家政大論集 Vol.3 No.-
완충액의 種類 및 光照射가 paraquat의 毒性低下에 미치는 影響을 검토한 結果 使用한 3種(sodium citrate, sodium phosphate, tris-HCl)의 완충액 中 sodium phosphate buffer에 paraquat를 용해하였을 때가 가장 큰 幅으로 毒性이 低下되었다. 經時的으로는 1日만에 約 60%의 細菌이 生育하였고 4日째 내는 95% 以上의 細菌이 生育함으로써 sodium phosphate buffer 中에서는 約 4日만에 paraquate의 毒性이 거의 消滅되었다. 한편, 暗所에 保有한 paraquate에 比하여 自然光구에서 1.3倍 紫外線 照射구에서 約 2倍의 生有율이 檢出되므로 光照射에 의하여 paraquat의 毒性이 보다 빨리 消滅되는 것으로 判斷되었다. Experiment was carried out to understand the effect or buffer solution and light irradiation on toxicity of paraquat against microorgnism. Paraquat most rapidly lost its toxicity in the sodium buffer solution among three kinds of tested solutions. (sodium citrate, sodium phosphate and tris-HCI) By previous incubation of paraquat in the solution for 24 hours, the organism showed about 60 percent of survival rate. The paraquat lost almost of its toxicity as showing at least 95 per cent of survival by continuous incubation for 4 days. The organism showed a survival rate of 1.3 times higher by natural light irradiation and 2.0 times higher by ultraviolet ray irradiation than control.
Guem San Lee,Eun Sook Kim,Su In Cho,Jung Hoon Kim,Goya Choi,Young Sung Ju,Sun Hwa Park,Seung Il Jeong,Hong Jun Kim 한국응용생명화학회 2010 Journal of Applied Biological Chemistry (J. Appl. Vol.53 No.3
7,9,2`,4`-Tetrahydroxy-8-isopentenyl-5-methoxychalcone (THIPMC), isolated from the roots of Sophora flavescens Ait., was found to be active against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE), either alone
( Guem San Lee ),( Eun Sook Kim ),( Su In Cho ),( Jung Hoon Kim ),( Go Ya Choi ),( Young Sung Ju ),( Sun Hwa Park ),( Seung Il Jeong ),( Hong Jun Kim ) 한국응용생명화학회 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.3
7,9,2`,4`-Tetrahydroxy-8-isopentenyl-5-methoxychalcone (THIPMC), isolated from the roots of Sophora flavescens Ait., was found to be active against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE), either alone or in combination with ampicillin (AM) or gentamicin (GM), vis checkerboard assay. Minimum inhibitory concentrations ranged from 1 to 8 μg/mL for THIPMC, from 128 to 1024 μg/mL for AM, and from 128 to 512 μg/mL for GM, respectively. The combinations of THIPMC plus AM or GM yielded a fractional inhibitory concentration index ranging from 0.188 to 0.375 μg/mL, thereby indicating a synergistic effect. These findings suggest that THIPMC alone or in combination with antibiotics against MRSA might be useful for controlling MRSA infections. However, VRE infection was only effectively treated by THIPMC alone.
Lee, Guem-San,Kim, Eun-Sook,Cho, Su-In,Kim, Jung-Hoon,Choi, Go-Ya,Ju, Young-Sung,Park, Sun-Hwa,Jeong, Seung-Il,Kim, Hong-Jun The Korean Society for Applied Biological Chemistr 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.3
7,9,2',4'-Tetrahydroxy-8-isopentenyl-5-methoxychalcone (THIPMC), isolated from the roots of Sophora flavescens Ait., was found to be active against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE), either alone or in combination with ampicillin (AM) or gentamicin (GM), vis checkerboard assay. Minimum inhibitory concentrations ranged from 1 to $8\;{\mu}g/mL$ for THIPMC, from 128 to $1024\;{\mu}g/mL$ for AM, and from 128 to $512\;{\mu}g/mL$ for GM, respectively. The combinations of THIPMC plus AM or GM yielded a fractional inhibitory concentration index ranging from 0.188 to $0.375\;{\mu}g/mL$, thereby indicating a synergistic effect. These findings suggest that THIPMC alone or in combination with antibiotics against MRSA might be useful for controlling MRSA infections. However, VRE infection was only effectively treated by THIPMC alone.
목통(木通),천목통(川木通),관목통(關木通)의 감별기준(鑑別基準)
이금산 ( Guem San Lee ),박경범 ( Kyoung Bum Park ),김영식 ( Young Sik Kim ),이승호 ( Seung Ho Lee ),오현민 ( Hyun Min Oh ),백지성 ( Ji Seong Baek ),조수인 ( Su In Cho ),김홍준 ( Hong Jun Kim ),주영승 ( Young Sung Ju ) 대한본초학회 2011 大韓本草學會誌 Vol.26 No.1
Objectives: Due to morphological similarity, many substitutes and counterfeits have been used as Moktong for thousands of years. This study was designed to determine discriminative criteria for identifying medicinal herbs used as Moktong. Methods: Akebia quinata, A. trifoliata, Clematis armandii, and Aristolochia manshuriensis were collected in various regions. Samples were authenticated and examined by observation and microscopy with paraffin embedding and triple staining using the modified Ju staining method. Results: Three different types of features to establish discriminative criteria for four kinds of Moktong were identified. Botanical features include differences in phyllotaxy, leaf shape, and caulescent. External morphological features were arrangement and size of fine holes(xylem), and overall shape of the section. Internal morphological features include the number of medullary rays, shape of the pitch, type of tissues rounding pitch, appearance of annual rings, shape and amount of crystals(calcium-oxalate), and the appearance of cork cambium. Further details( e.g. identification keys) are in the article. Conclusions: These criteria could provide an effective method for identifying numerous kinds of Moktong distributed in markets throughout northeast Asian nations.
이금산 ( Guem San Lee ),김정훈 ( Jung Hoon Kim ),최고야 ( Go Ya Choi ),강대훈 ( Dae Hoon Kang ),황성연 ( Sung Yeoun Hwang ),정승일 ( Seung Il Jeong ),김홍준 ( Hong Jun Kim ),주영승 ( Young Sung Ju ) 대한본초학회 2008 大韓本草學會誌 Vol.23 No.4
Objectives: To determine the standards for discrimination of Magnoliae Cortex, the experiment of specific external-internal characters and the physicochemical pattern analysis were performed. Methods: External characteristics was observed using a stereoscope. Paraffin-mediated sectioned materials were stained by Ju`s method. Physicochemical patterns of materials were analyzed using HPLC. Results: 1. Botanical characteristics: Magnolia officinalis had one seed and a white flower, while M. obovata had two seeds and a white flower. Machilus thunbergii had berry and spherical fruits and yellowish green panicles. 2. External characteristics: M. officinalis and M. obovata were dark and thick. M. officinalis was gray brown and greasy while M. obovata was light-gray, less oily and smoothly sectioned. Machilus thunbergii was thin and relatively light or yellow-brown, coarsely sectioned and faintly specific scents. 3. Internal characteristics: The bast parts of M. officinalis and M. obovata were commonly wider than Machilus thunbergii. The cork cortex of M. officinalis was 10~mg/L cell layers with many oil cells, while that of M. obovata was 4~7 cell layers with less oil cells. Machilus thunbergiis xylem which consisted of ring-shaped cambium at 1st and 2nd part was occupied in large portion. 4. Physicochemical pattern: Both M. officinalis and M. obovata involved honokiol and magnolol. All kinds of M. officinalis involved Magnatriol B but one kind of M. ovobata and all of Machilus thunbergii didn`t. Machilus thunbergii showed different pattern of chromatogram from that of 2 species above. Conclusions: These results could be used as standards for discrimination of Magnoliae Cortex and as the method of objectification in medicinal herbs giving the basic resource for bioactivity research.
Kim, Hyun Jung,Lee, Guem San,Kim, Hyungwoo,Kim, Byung Joo WJG Press 2017 WORLD JOURNAL OF GASTROENTEROLOGY Vol.23 No.29
<P><B>AIM</B></P><P>To investigate the effects of a water extract of Hwangryunhaedok-tang (HHTE) on the pacemaker potentials of mouse interstitial cells of Cajal (ICCs).</P><P><B>METHODS</B></P><P>We dissociated ICCs from small intestines and cultured. ICCs were immunologically identified using an anti-c-kit antibody. We used the whole-cell patch-clamp configuration to record the pacemaker potentials generated by cultured ICCs under the current clamp mode (<I>I</I> = 0). All experiments were performed at 30 °C-32 °C</P><P><B>RESULTS</B></P><P>HHTE dose-dependently depolarized ICC pacemaker potentials. Pretreatment with a 5-HT<SUB>3</SUB> receptor antagonist (Y25130) or a 5-HT<SUB>4</SUB> receptor antagonist (RS39604) blocked HHTE-induced pacemaker potential depolarizations, whereas pretreatment with a 5-HT<SUB>7</SUB> receptor antagonist (SB269970) did not. Intracellular GDPβS inhibited HHTE-induced pacemaker potential depolarization and pretreatment with a Ca<SUP>2+</SUP>-free solution or thapsigargin abolished the pacemaker potentials. In the presence of a Ca<SUP>2+</SUP>-free solution or thapsigargin, HHTE did not depolarize ICC pacemaker potentials. In addition, HHTE-induced pacemaker potential depolarization was unaffected by a PKC inhibitor (calphostin C) or a Rho kinase inhibitor (Y27632). Of the four ingredients of HHT, Coptidis Rhizoma and Gardeniae Fructus more effectively inhibited pacemaker potential depolarization.</P><P><B>CONCLUSION</B></P><P>These results suggest that HHTE dose-dependently depolarizes ICC pacemaker potentials through 5-HT<SUB>3</SUB> and 5-HT<SUB>4</SUB> receptors <I>via</I> external and internal Ca<SUP>2+</SUP> regulation and <I>via</I> G protein-, PKC- and Rho kinase-independent pathways.</P>
Cinnamic acid, Cinnamaldehyde의 부위별 정량을 통한 계지(桂枝)의 음편(飮片) 규격 연구
김영식 ( Young Sik Kim ),이금산 ( Guem San Lee ),김정훈 ( Jung Hoon Kim ),최고야 ( Go Ya Choi ),정승일 ( Seung Il Jeong ),조수인 ( Su In Cho ),주영승 ( Young Sung Ju ),김홍준 ( Hong Jun Kim ) 대한본초학회 2011 大韓本草學會誌 Vol.26 No.2
Objectives: In the present study, the contents of cinnamic acid and cinnamaldehyde in three different parts of Cinnamomi Ramulus (CR) (the whole body, the bark part, and the wood part) was evaluated using UPLC (ultra performance liquid chromatography) in order to investigate a suitable cutting method. Methods: Analysis was performed on SMART LC with UV detector. Reference compounds were separated on Inertsil ODS-4 column (2.1 mm × 50 mm, 3 μm, GL Science, Japan) using isolation elution with water and acetonitrile each containing acetic acid at a flow rate of 500 μL/min. Additionally, samples of CR were purchased from pharmacy of medicinal herb. Results: The correlation coefficients of the cinnamic acid and cinnamaldehyde levels showed good linearity (r2 ≥ 0.9999) over the linear ranges. Furthermore, the bark part exhibited higher concentration levels of reference compounds than the wood part in all samples. In addition the bark exfoliation rates in oblique and perpendicular-long cut samples of CR were lower than the perpendicular-short cut samples. Conclusions: These results suggested that the optimal cutting method would be able to reduce the bark exfoliation. Therefore, the oblique or perpendicular-long cutting method is considered to be a better cutting type than the perpendicular-short cutting method.
Multiplex PCR을 이용한 4 종류 목향(木香)의 감별
도의정 ( Eui Jeong Doh ),이금산 ( Guem San Lee ),주영승 ( Young Sung Ju ),오승은 ( Seung Eun Oh ) 대한본초학회 2014 大韓本草學會誌 Vol.29 No.3
Objectives : Aucklandiae Radix (Muxiang) one of important herbal medicines in oriental medicine, is defined as the dried root of Aucklandia lappa (Asteraceae). Owing to the similarities in the morphology and name, Inulae Radix (Tu-Muxiang) and Vladimiriae Radix (Chuan-Muxiang) as well as Aristolochiae Radix (Qing-Muxiang) originated from other medicinal plants are often used as substitutes and/or adulterants of Aucklandiae Radix. Therefore, a reliable authentication of these herbal medicines is necessarily for the public health and prevention of misuse. Methods : 32 samples of medicinal plants supplying Aucklandiae Radix, Inulae Radix, Vladimiriae Radix, and Aristolochiae Radix were collected in Korea and China. The ITS (Internal transcribed spacer) nucleotide sequences of samples were determined. The PCR primers to amply DNA marker of each herbal medicine were designed basing on the specific ITS regions showing differences in the sequences among medicinal plants. Results : Primer set Al R/IS F designed in this work amplified 220 bp PCR product only in samples of Aucklandiae Radix. In contrast, primer set Ih F/IS R, Vs R/IS F, and AcR F1/Ac R amplified 250 bp product, 356 bp prouct, and 516 bp product respectively to identify Inulae Radix, Vladimiriae Radix, and Aristolochiae Radix. Conclusions : The primers designed basing on the nucleotide sequences of ITS regions appearing differenced in the sequences among medicinal plants amplified the DNA markers for the identification of Aucklandiae Radix, Inulae Radix, Vladimiriae Radix, and Aristolochiae Radix. These herbal medicines were more efficiently identified by multiplex PCR method using all primers in a single PCR process.