Genetics and Breeding for Modified Fatty Acid Profile in Soybean Seed Oil

James Grover Shannon 한국작물학회 2007 Journal of crop science and biotechnology Vol.10 No.4

Soybean [Glycine max (L.) Merr.] oil is versatile and used in many products. Modifying the fatty acid profile would make soy oil more functional in food and other products. The ideal oil with the most end uses would have saturates (palmitic + stearic acids) reduced from 15 to < 7%, oleic acid increased from 23 to > 55%, and linolenic acid reduced from 8 to < 3%. Reduced palmitic acid (16:0) is conditioned by three or more recessive alleles at the Fap locus. QTLs for reduced palmitic acid have mapped to linkage groups (LGs) A1, A2, B2, H, J, and L. Genes at the Fad locus control oleic acid content (18:1). Six QTLs (R2 = 4-25%) for increased 18:1 in N00-3350 (50 to 60% 18:1) explained four to 25% of the phenotypic variation. M23, a Japanese mutant line with 40 to 50% 18:1 is controlled by a single recessive gene, ol. A candidate gene for FAD2-1A can be used in marker-assisted breeding for high 18:1 from M23. Low linolenic acid (18:3) is desirable in soy oil to reduce hydrogenation and trans-fat accumulation. Three independent recessive genes affecting omega-3 fatty acid desaturase enzyme activity are responsible for the lower 18:3 content in soybeans. Linolenic acid can be reduced from 8 to about 4, 2 ,and 1% from copies of one, two, or three genes, respectively. Using a candidate gene approach perfect markers for three microsomal omega-3 desaturase genes have been characterized and can readily be used in for marker assisted selection in breeding for low 18:3. Soybean [Glycine max (L.) Merr.] oil is versatile and used in many products. Modifying the fatty acid profile would make soy oil more functional in food and other products. The ideal oil with the most end uses would have saturates (palmitic + stearic acids) reduced from 15 to < 7%, oleic acid increased from 23 to > 55%, and linolenic acid reduced from 8 to < 3%. Reduced palmitic acid (16:0) is conditioned by three or more recessive alleles at the Fap locus. QTLs for reduced palmitic acid have mapped to linkage groups (LGs) A1, A2, B2, H, J, and L. Genes at the Fad locus control oleic acid content (18:1). Six QTLs (R2 = 4-25%) for increased 18:1 in N00-3350 (50 to 60% 18:1) explained four to 25% of the phenotypic variation. M23, a Japanese mutant line with 40 to 50% 18:1 is controlled by a single recessive gene, ol. A candidate gene for FAD2-1A can be used in marker-assisted breeding for high 18:1 from M23. Low linolenic acid (18:3) is desirable in soy oil to reduce hydrogenation and trans-fat accumulation. Three independent recessive genes affecting omega-3 fatty acid desaturase enzyme activity are responsible for the lower 18:3 content in soybeans. Linolenic acid can be reduced from 8 to about 4, 2 ,and 1% from copies of one, two, or three genes, respectively. Using a candidate gene approach perfect markers for three microsomal omega-3 desaturase genes have been characterized and can readily be used in for marker assisted selection in breeding for low 18:3.

Molecular breeding for oil composition in soybean

J. Grover Shannon,Jeong Dong Lee,Kristen D. Bilyeu 한국육종학회 2007 한국육종학회 학술발표회 발표요지 Vol.39 No.1

종간교잡 유래 콩 극소립 재조합 자식계통의 농업적인 특성

이정동,정연신,Shannon J. Grover,박순기,정명근,황영현 한국육종학회 2005 한국육종학회지 Vol.37 No.5

Wild Soybean (Glycine soja Sieb. & Zucc) -A Genetic Source for Soybean Variety Improvement

( Jeong Dong Lee ),( J. Grover Shannon ),( Gyu Hwa Chung ),( Young Hyun Hwang ) 한국콩연구회 2011 韓國콩硏究會誌 Vol.28 No.1

Wild soybean (Glycine, soja Sieb. & Zucc) is an important source of genetic variation to introduce useful traits into cultivated soybeans [Glycine max (L.) Merr.]. Wild soybean is widely distributed in Eastern Asia including, Korea, China, Japan, and other countries. Soybean breeders and geneticists are interested discovering useful genes from G. soja to introduce into cultivated soybean for variety improvement. Previous reports have shown that G. soja is a good source to find new and useful genes for resistance to biotic and abiotic stresses, improved seed composition, and agronomic characteristics such as yield for soybean variety improvement. Wild soybean populations at present are in decline and have a higher risk of extinction because human activities are infringing on and destroying the natural habitats of G. soja. Therefore, to preserve the genetic diversity and useful traits found in G. soja, it is necessary to collect and conserve accessions from unexplored regions native to wild soybeans as a valuable resource for soybean variety improvement.

The Current Status of Forage Soybean

( Sovetgul Asekova ),( J Grover Shannon ),( Jeong Dong Lee ) 한국육종학회 2014 Plant Breeding and Biotechnology Vol.2 No.4

Soybeans have a long history as a nutritious hay and silage crop. Early research extensively investigated forage yield, adaptability to various maturity zones, and nutritional values. Evaluation and breeding with diverse soybean accessions continued to optimize soybean forage yield and quality. There is still interest by breeders in developing more desirable forage soybeans, depending on market demand, and the existing interests of crop and livestock producers. In this review, we provide an update compiled from recent publications on the use and development of soybean as a forage crop.

Selection for protein content in soybean from single F2 seed by near infrared reflectance spectroscopy : Selection protein by NIR in soybean

Lee, Jeong-Dong,Shannon, J. Grover,Choung, Myoung-Gun Springer-Verlag 2010 Euphytica Vol.172 No.1

Genetics and Breeding for Modified Fatty Acid Profile in Soybean Seed Oil

Lee, Jeong-Dong,Bilyeu, Kristin D.,Shannon, James Grover The Korean Society of Crop Science 2007 Journal of crop science and biotechnology Vol.10 No.4

Soybean [Glycine max(L.) Merr.] oil is versatile and used in many products. Modifying the fatty acid profile would make soy oil more functional in food and other products. The ideal oil with the most end uses would have saturates(palmitic + stearic acids) reduced from 15 to < 7%, oleic acid increased from 23 to > 55%, and linolenic acid reduced from 8 to < 3%. Reduced palmitic acid(16:0) is conditioned by three or more recessive alleles at the Fap locus. QTLs for reduced palmitic acid have mapped to linkage groups(LGs) A1, A2, B2, H, J, and L. Genes at the Fad locus control oleic acid content(18:1). Six QTLs($R^2$=4-25%) for increased 18:1 in N00-3350(50 to 60% 18:1) explained four to 25% of the phenotypic variation. M23, a Japanese mutant line with 40 to 50% 18:1 is controlled by a single recessive gene, ol. A candidate gene for FAD2-1A can be used in marker-assisted breeding for high 18:1 from M23. Low linolenic acid(18:3) is desirable in soy oil to reduce hydrogenation and trans-fat accumulation. Three independent recessive genes affecting omega-3 fatty acid desaturase enzyme activity are responsible for the lower 18:3 content in soybeans. Linolenic acid can be reduced from 8 to about 4, 2, and 1% from copies of one, two, or three genes, respectively. Using a candidate gene approach perfect markers for three microsomal omega-3 desaturase genes have been characterized and can readily be used in for marker assisted selection in breeding for low 18:3.

Altering Fatty Acid Composition for Utilization in Food an Industry for Soybean Oil

( Kristin Bilyeu ),( Anh Pham ),( J. Grover Shannon ),( Jeong Dong Lee ) 한국콩연구회 2011 韓國콩硏究會誌 Vol.28 No.1

The fatty add profile of vegetable oil determines the ultimate utilization that the oil is most suited for in both food applications and for industrial uses. We recently created soybean seeds containing a high oleic add content in the oil, a trait that will improve the oxidative stability of the oil and offer the ability to replace the functionality of partially hydrogenated soybean oil without the production of trans fatty acids. This review collates the information necessary to produce a more functional soybean oil, specifically one with high oleic acid and low linolenic acid contents. The research demonstrated that combinations of three or four mutant desaturase genes, obtained independently from sources including natural variants as well as induced mutants, could produce soybean oil with the desired fatty acid profiles, depending to some degree on the environment used for seed production. The impact of this research is the advancement of understanding the target gene combinations to develop more functional soybean oils.

Genetic mapping of quantitative trait loci conditioning salt tolerance in wild soybean (Glycine soja) PI 483463

Ha, Bo-Keun,Vuong, Tri D.,Velusamy, Vijayan,Nguyen, Henry T.,Grover Shannon, J.,Lee, Jeong-Dong Springer-Verlag 2013 Euphytica Vol.193 No.1

Selection for soyabeans with high and environmentally stable lutein concentrations

Dhakal, Krishna Hari,Choung, Myoung-Gun,Hwang, Young-Sun,Fritschi, Felix B.,Shannon, J. Grover,Lee, Jeong-Dong Cambridge University Press 2014 Plant genetic resources Vol.12 No.1

<P>Lutein has significant nutritional benefits for human health. Therefore, enhancing soybean lutein concentrations is an important breeding objective. However, selection for soybeans with high and environmentally stable lutein concentrations has been limited. The objectives of this study were to select soybeans with high seed lutein concentrations and to determine the stability of lutein concentrations across environments. A total of 314 genotypes were screened and 18 genotypes with high lutein concentrations and five genotypes with low lutein concentrations were selected for further examination. These 23 genotypes and two check varieties were evaluated under six environments (two planting dates for 2 years at one location and two planting dates for 1 year at another location). Lutein concentrations were influenced by genotype, environment and genotype × environment interactions. Genotypes with late maturity and low lutein concentrations were more stable than those with early maturity and high concentrations. Early (May) planting resulted in greater lutein concentrations than late (June) planting. Among the genotypes evaluated, PI603423B (7.7 μg/g) and PI89772 (5.8 μg/g) had the greatest mean lutein concentrations and exhibited medium and high stability across the six environments, respectively. Thus, these genotypes may be useful for breeding soybeans with high and stable seed lutein concentrations.</P>