Laboratory and field performance of Trichopria drosophilae reared on Drosophila melanogaster and D. Suzukii

Gonzalez-Cabrera Jaime,Moreno-Carrillo Gabriel,Lopez-Juarez Alejandro A.,Anguiano-Amezcua Juan A.,Sanchez-Gonzalez Jorge A.,Mendoza-Ceballos Mario Y.,Arredondo-Bernal Hugo C. 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2

Two colonies of the parasitoid Trichopria drosophilae (Perkins) (Hymenoptera: Diapriidae) are reproduced using a mass rearing approach in Mexico. The first colony uses the worldwide commercial host Drosophila melanogaster Meigen (Diptera: Drosophilidae) reared on an artificial diet (=T. drosophilae-Dm), while the second colony uses D. suzukii Matsumura reared on fresh banana (=T. drosophilae-Ds). Since theoretically the type of colony host affects parasitoid field performance, we conducted concatenated laboratory and field studies of both parasitoid lines. In the laboratory trials, T. drosophilae-Ds exhibited greater longevity, larger adult size, and higher fecundity than T. drosophilae-Dm. Both lines of parasitoids oviposited indistinctly in three drosophilid species commonly found in the berry zones of Colima: D. melanogaster, D. suzukii, and Zaprionus indianus Gupta. In the field trials, T. drosophilae-Ds had a larger population size and provided 2.4-fold higher pest reduction compared to T. drosophilae-Dm. Under the particular conditions of these field trials (berry fields separate from each other ≤ 3 km, and intensive agronomic management), these results demonstrate that T. drosophilae-Ds can significantly contribute more to the integrated management of D. suzukii than when reared on the commercial host D. melanogaster.

A Pathophysiological Perspective on COVID-19's Lethal Complication: From Viremia to Hypersensitivity Pneumonitis-like Immune Dysregulation

Marcos A. Sanchez-Gonzalez,Dave Moskowitz,Priya D. Issuree,George Yatzkan,Syed A. A. Rizvi,Kenneth Day 대한감염학회 2020 Infection and Chemotherapy Vol.52 No.3

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the coronavirus responsible for our recent coronavirus disease 2019 pandemic, is driving a lung immunopathology that strongly resembles a severe form of hypersensitivity pneumonitis (HP). A review of recent Severe acute respiratory syndrome-related coronavirus (SARS-CoV) and SARS-CoV-2 medical reports, as well as described characteristics of HP, lead us to postulate a theory for SARS-CoV-2 severe disease. We propose that the novel SARS-CoV-2 can act as a trigger and substrate of an HP-like severe immune reaction especially in genetically vulnerable individuals in addition to those with immune senescence and dysregulation. Accordingly, the purpose of our letter is to shift the emphasis of concern surrounding immune activity from viral infection to an HP-like severe immune reaction. We review similarities in disease presentation between infection and allergy, relevant immunopathology, and outline phases of SARS-CoV-2 disease with perspectives on therapy and critical care. Altogether, the favored course is to begin treatments that address the disease at the earliest phase before immune dysregulation leading to uncontrolled pulmonary inflammation.

Increased Expression of HOXB2 and HOXB13 Proteins is Associated with HPV Infection and Cervical Cancer Progression

Gonzalez-Herrera, A.L.,Salgado-Bernabe, M.,Velazquez-Velazquez, C.K.,Salcedo-Vargas, M.,Andrade-Manzano, A.,Avila-Moreno, F.,Pina-Sanchez, P. Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.4

Background: Cervical cancer (CeCa) is the second most common cancer in women in developing countries, and human papilloma virus (HPV) is the primary etiological factor. Aberrant expression of HOX transcription factors has been observed in several types of cancer. To date, however, no reports exist on the expression of HOXB2 and HOXB13 proteins during neoplastic progression in CeCa and its correlation with HPV infection. Materials and Methods: Expression of HOXB2 and HOXB13 proteins was assessed in tissue microarrays from normal cervical epithelium, cervical intraepithelial neoplasias grade 1-3, and CeCa. HPV was detected by PCR and sequencing. Expression of HOX-positive cells was determined in each diagnostic group. Results: Percentage of HOXB2- and HOXB13-positive cells gradually increased from means of 10.9% and 16.7%, respectively, in samples from healthy women, to 75.2% and 88.6% in those from CeCa patients. Frequency of HPV infection also increased from 13% in healthy tissue samples to 92.3% in CeCa. Both HOXB2 and HOXB13 proteins were preferentially expressed in HPV+ samples. Conclusions: The present study represents the first report on the expression of both HOXB2 and HOXB13 proteins through cervix tumorigenesis, providing evidence that increased expression of such proteins is a common event during progression to CeCa.

Sources, Characteristics, and Therapeutic Applications of Mesenchymal Cells in Tissue Engineering

Gonzalez-Vilchis Rosa Angelica,Piedra-Ramirez Angelica,Patiño-Morales Carlos Cesar,Sanchez-Gomez Concepcion,Beltran-Vargas Nohra E. 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.2

Tissue engineering (TE) is a therapeutic option within regenerative medicine that allows to mimic the original cell environment and functional organization of the cell types necessary for the recovery or regeneration of damaged tissue using cell sources, scaffolds, and bioreactors. Among the cell sources, the utilization of mesenchymal cells (MSCs) has gained great interest because these multipotent cells are capable of differentiating into diverse tissues, in addition to their self-renewal capacity to maintain their cell population, thus representing a therapeutic alternative for those diseases that can only be controlled with palliative treatments. This review aimed to summarize the state of the art of the main sources of MSCs as well as particular characteristics of each subtype and applications of MSCs in TE in seven different areas (neural, osseous, epithelial, cartilage, osteochondral, muscle, and cardiac) with a systemic revision of advances made in the last 10 years. It was observed that bone marrow-derived MSCs are the principal type of MSCs used in TE, and the most commonly employed techniques for MSCs characterization are immunodetection techniques. Moreover, the utilization of natural biomaterials is higher (41.96%) than that of synthetic biomaterials (18.75%) for the construction of the scaffolds in which cells are seeded. Further, this review shows alternatives of MSCs derived from other tissues and diverse strategies that can improve this area of regenerative medicine.

Contact System Method for the Precise Interaction Between Cobots and Mobile Robots in Smart Manufacturing

Ivan Sanchez-Calleja,Alberto Martínez-Gutierrez,Ruben Ferrero-Guillén,Javier Díez-Gonzalez,Hilde Perez 한국정밀공학회 2024 International Journal of Precision Engineering and Vol.25 No.2

The advent of smart manufacturing (SM) has led to the creation of collaborative environments with cyber-physical systems (CPS) that generate added value. However, the performance of combined industrial operations between mobile CPS such as autonomous mobile robots (AMRs) and collaborative robots (cobots) is hampered by the high uncertainty between their relative spatial locations and the existence of heterogeneous communication protocols that create a barrier to their integration into production processes. For this reason, a novel contact system method (CSM) is proposed to determine the position of the AMR without the need for any additional hardware making use of an architecture that facilitates efficient communication between AMRs and cobots. For this purpose, a mathematical model has been defined to characterize the position of a spatial object with six degrees of freedom in order to calculate the deviation between the AMR and the cobot base. The proposed method has also been evaluated by quantifying the position and orientation error before and after applying the CSM. The effectiveness of the CSM method has been assessed in a real application case based on the feasibility of performing an assembly operation between a bearing and different shafts. The results show a significant improvement of 96.2% in positional accuracy and 85.4% in orientation compared to AMR accuracy. In addition, a 92.5% success rate was achieved in the assembly operation analyzed between a bearing and a shaft of the same diameter. Furthermore, the proposed architecture has enabled the coordination between the cobot and the AMR by automating the processes. Therefore, this work contributes to the field of SM by proposing a practical solution to the challenges of generating added value through the creation of collaborative environments with CPS.

Angiotensin-Converting Enzyme Inhibitors and Angioedema

Mario Sanchez-Borges,Luis A Gonzalez-Aveledo 대한천식알레르기학회 2010 Allergy, Asthma & Immunology Research Vol.2 No.3

Purpose: To investigate the incidence and clinical characteristics of angioedema associated with the use of angiotensin-converting enzyme inhibitors (ACEIs) in an outpatient allergy department. Methods: A retrospective review of medical records of new patients seen in an allergy clinic. Demographic and clinical data of patients with ACEI-induced angioedema were analyzed. Results: Nine (0.37%) out of 2,421 new patients attending the allergy clinic developed ACEI-associated angioedema. Enalapril was the drug most frequently incriminated. The onset of the angioedema was as early as after the first dose or as late as 2 years after beginning treatment. Six patients experienced life-threatening angioedema involving the tongue, oropharynx, or larynx, and two patients required transfer to the intensive care unit. One patient required a tracheostomy. Conclusions: Angiotensin-converting enzyme inhibitor treatment is often responsible for angioedema, especially involving the upper airways. Due to the high proportion of the population exposed to ACEIs and to the severity of this adverse effect, it is important that physicians consider ACEIs as possible inducers when evaluating patients with acute or recurrent angioedema.

EXPERIMENTAL DIETS AS ALTERNATIVE AGAINST Vibrio parahaemolyticus STRAIN (EMS/AHPND+) IN WHITE SHRIMP Litopenaeus vannamei IN THE STATE OF SONORA, MEXICO

Salguero-Gonzalez, M.A.,Sanchez-Diaz, R.,Ibarra-Gamez, J.C. 한국수산과학회 양식분과 2015 한국수산과학회 양식분과 학술대회 Vol.2015 No.5

Power Absorption Measurements during NMR Experiments

N. Felix-Gonzalez,A. L. Urbano-Bojorge,C. Sanchez-L de Pablo,V. Ferro-Llanos,F. del Pozo-Guerrero,J. J. Serrano-Olmedo 한국자기학회 2014 Journal of Magnetics Vol.19 No.2

The heating produced by the absorption of radiofrequency (RF) has been considered a secondary undesirable effect during MRI procedures. In this work, we have measured the power absorbed by distilled water, glycerol and egg-albumin during NMR and non-NMR experiments. The samples are dielectric and examples of different biological materials. The samples were irradiated using the same RF pulse sequence, whilst the magnetic field strength was the variable to be changed in the experiments. The measurements show a smooth increase of the thermal power as the magnetic field grows due to the magnetoresistive effect in the copper antenna, a coil around the probe, which is directly heating the sample. However, in the cases when the magnetic field was the adequate for the NMR to take place, some anomalies in the expected thermal powers were observed: the thermal power was higher in the cases of water and glycerol, and lower in the case of albumin. An ANOVA test demonstrated that the observed differences between the measured power and the expected power are significant.

Evaluation of Antimicrobial Activities of Sequential Spray Applications of Decontamination Treatments on Chicken Carcasses

Benli, Hakan,Sanchez-Plata, Marcos X.,Ilhak, Osman Irfan,De Gonzalez, Maryuri T. Nunez,Keeton, Jimmy T. Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.3

The objective of this study was to evaluate the effects of sequential applications of ${\varepsilon}$-polylysine (EPL) or lauramide arginine ethyl ester (LAE) sprays followed by an acidic calcium sulfate (ACS) spray on inoculated chicken carcasses to reduce Salmonella (Salmonella enterica serovars including Salmonella typhimurium and Salmonella enteritidis) contamination during 6 days of storage ($4.4^{\circ}C$). Secondly, reductions of the resident microflora were studied on uninoculated chicken carcasses following the sequential application of the treatments, chilling and 10 days of storage at $4.4^{\circ}C$. The treatment of Salmonella inoculated carcasses with 300 mg/L EPL followed by 30% ACS (EPL300-ACS30) sprays reduced Salmonella counts initially by 1.5 log cfu/mL and then by 1.2 log cfu/mL (p<0.05) following 6 days of storage at $4.4^{\circ}C$. Likewise, 200 mg/L LAE followed by 30% ACS (LAE200-ACS30) treatment reduced initial Salmonella counts on poultry carcasses by 1.8, 1.4 and 1.8 log cfu/mL (p<0.05), respectively, after 0, 3, and 6 days storage. Immediately after the treatments, EPL300-ACS30 and LAE200-ACS30 both reduced Escherichia coli counts significantly by 2.6 and 2.9 log cfu/mL, respectively. EPL300-ACS30 and LAE200-ASC30 were effective in lowering psychrotroph counts by 1 log cfu/mL on day 10 when compared to the control and distilled water treatments. This study demonstrated that EPL300-ACS30 and LAE200-ACS30 were effective in reducing Salmonella on inoculated chicken carcasses both after treatment and during the storage at $4.4^{\circ}C$ for up to 6 days. In addition, reductions in psychrotroph counts indicated that these treatments might have the potential to increase the shelf-life of poultry carcasses.

Unexpected Presence of Blastocystis Subtype 1-3 DNA in Human Vaginal and Sperm Samples Coinfected with Trichomonas vaginalis

Villalobos, Guiehdani,Sanchez-Aguillon, Fabiola,Carmona-Maldonado, Marcia Veronica,Gonzalez-Arenas, Nelly Raquel,Lopez-Escamilla, Eduardo,Hernandez-Castro, Rigoberto,Romero-Valdovinos, Mirza,Martinez- The Korea Society for Parasitology and Tropical Me 2022 The Korean Journal of Parasitology Vol.60 No.3

There have been few reports on extra-enteric infections by Blastocystis STs and none have been molecularly identified in samples from human reproductive organs. We report for the first time the identification of 3 different subtypes of Blastocystis (ST1-3) in vaginal and sperm samples, from patients infected with Trichomonas vaginalis. Blastocystis STs were identified by PCR-sequencing and by phylogenetic inferences using 28 vaginal swab samples and 7 sperm samples from patients trichomoniasis. Blastocystis STs were identified in 6 of 28 vaginal swabs (21.4%) and in 3 of 7 sperm samples (42.8%). In both biological samples, STs 1-3 were found; one vaginal sample showed subtype co-infection with ST1 and ST3. High genetic variation was observed in the sequences obtained and no specific clustering in the phylogenetic trees was detected. Most of the haplotypes identified were placed far from the main dispersal centers. Our finding suggested that incorrect cleaning of the genital area or a contamination by combination of anal and vaginal intercourse.