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Cloning and Biochemical Characterization of a Hyaluronate Lyase from Bacillus sp. CQMU-D
Wang Lu,Liu Qianqian,Gong Xue,Jian Wenwen,Cui Yihong,Jia Qianying,Zhang Jibei,Zhang Yi,Guo Yanan,Lu He,Tu Zeng 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.2
Hyaluronidase (HAase) can enhance drug diffusion and dissipate edema by degrading hyaluronic acid (HA) in the extracellular matrix into unsaturated HA oligosaccharides in mammalian tissues. Microorganisms are recognized as valuable sources of HAase. In this study, a new hyaluronate lyase (HAaseD) from Bacillus sp. CQMU-D was expressed in Escherichia coli BL21, purified, and characterized. The results showed that HAaseD belonged to the polysaccharide lyase (PL) 8 family and had a molecular weight of 123 kDa. HAaseD could degrade chondroitin sulfate (CS) -A, CS-B, CSC, and HA, with the highest activity toward HA. The optimum temperature and pH value of HAaseD were 40°C and 7.0, respectively. In addition, HAaseD retained stability in an alkaline environment and displayed higher activity with appropriate concentrations of metal ions. Moreover, HAaseD was an endolytic hyaluronate lyase that could degrade HA to produce unsaturated HA oligosaccharides. Together, our findings indicate that HAaseD from Bacillus sp. CQMU-D is a new hyaluronate lyase and with excellent potential for application in industrial production.
Guoliang Fan,Liu Zhao,Cairong Gong,Jia Ma,Gang Xue 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.1
Copper catalysts prepared using two kinds of supports (BaTiO3 and Fe2O3@BaTiO3) have been tested for soot combustion. Perovskite-type oxide BaTiO3 shows excellent properties as support but has small specific surface area. Fe2O3@BaTiO3 core/shell microspheres, as improved support of BaTiO3 support, were fabricated by a hydrothermal-annealing approach. Fe2O3@BaTiO3 support has large specific surface area, which optimizes the contact condition of the gassoot-catalyst three-phase reaction. Due to the Cu-Fe2O3@BaTiO3 interaction, the redox properties of copper are improved. Soot combustion is tested under O2 gas flow and NOx/O2 gas flow, and Fe2O3@BaTiO3 supported copper catalyst (Cu/Fe2O3@BaTiO3) all shows higher catalytic activity and CO2 selectivity than BaTiO3 supported copper catalyst (Cu/BaTiO3).