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Tumor Suppressor Gene Therapy Strategies in Human Brain Tumor
Gomez-Manzano, Candeleria,Kyritsis, Athanassios,Alfred Yung, W. K.,Fueyo, Juan 가톨릭 의과학연구원 1997 가톨릭 의과학연구원 국제학술대회 Vol.1 No.-
Wild-type p53 is involved in several aspects of cell cycle control and suppression of transformation. inducing either apoptosis or G block in cell cycle progression. Using a recombinant adenovirus containting the wild-type p53 cDNA, the biological effects of the newly expressed wild-type p53 protein were examined in six human glioma cell lines. Three cell lines(U-251 MG. U0373 MG. and A-172) expressed endogenous mutant p53. and the other three(U-87 MG EFC-2, and D54 MG) expressed wild-type p53. The restoration of normal p53-encoded protein in the mutant cell lines induecd apoptosis as assessed by morphological studies using nuclear staining. electron microscopy. and flow cytometric assays. In wild-type p53 cell lines. However, the over expression of wild-type p53 did not result in apoptosis but inhibited cellular proliferation rather drastically and modified the neoplastic phenotype. This study shows that over-expression of wild-type p53 protein by an adenoviral vector induces apoptosis in mutant glioma cells as well as growth arrest in wild-type glioma cells.