Prognostic Role of PTEN Gene Expression and Length of Survival of Breast Cancer Patients in the North East of Iran

Golmohammadi, Rahim,Rakhshani, Mohammad Hassan,Moslem, Ali Reza,Pejhan, Akbar Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup3

PTEN protein is an important tumour suppressor factor detectable by immunohistochemistry. The goal of the present study was to investigate the prognostic role of PTEN gene expression focusing on length of survival in breast cancer patients. This descriptive-analytical study was conducted on 100 breast cancer cases referred to Sabzevar hospitals in the north east of Iran between 2010 and 2011, followed up to 2015. The PTEN gene expression of tumour tissue samples was determined using specific monoclonal antibodies. The data were analyzed using Chi-square test and Fisher's exact test. Patient length of survival was analyzed after 4 years of follow-up using the Cox regression model. The PTEN gene was expressed in 70 of 100 samples, while being found at a high level in all noncancerous samples. There was an inverse significant relationship between expression of PTEN and tumour stage and grade (p<0.001). In addition, expression of PTEN in invasive ductal tumours was less than in non-invasive tumours. There was also an inverse significant relationship between the likelihood of death and PTEN gene expression (p<0.01). These findings indicate that lack of PTEN gene expression can be sign for a worse prognosis and poor survival in breast cancer.

Modeling and Analyzing One Vendor-Multiple Retailers VMI SC Using Stackelberg Game Theory

Golmohammadi, Amir-Mohammad,Javid, Negar Jahanbakhsh,Poursoltan, Lily,Esmaeeli, Hamid Korean Institute of Industrial Engineers 2016 Industrial Engineeering & Management Systems Vol.15 No.4

Game theory is a powerful tool for analyzing the Supply chain (SC) with different conflicting elements. Among them, the Stackelberg game is the one in which a player as leader has more power than the other ones as followers. Since in many SC systems one element has, in essence, more power than the others; the Stackelberg game has found many applications in SC studies. In this paper, we apply the Stackelberg game-theoretic approach and the corresponding equilibrium point to formulate and analyze a two echelon VMI SC. Comprehensive computational results on an experimental case are conducted to numerically analyze the performance of VMI system against three groups of critical parameters. Moreover, a critical comparison demonstrates the poorer performance of decentralized VMI system than centralized one. This naturally necessitates designing proper contracts between VMI partners in order to more effectively implement the realistic decentralized system.

Prognostic Role of PTEN Gene Expression in Breast Cancer Patients from North-East Iran

Golmohammadi, Rahim,Rakhshani, Mohammad Hassan,Moslem, Ali Reza,Pejhan, Akbar Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.9

Background: PTEN protein is one of the most important tumour suppressor factors which is detectable by immunohistochemistry. The goal of the present study was to investigate the prognostic role of PTEN gene expression in breast cancer patients. Materials and Methods: This descriptive-analytical study was conducted on 100 breast cancer patients referred to Sabzevar hospitals in the north-east of Iran between 2010 and 2011, who were followed up to 2015. PTEN gene expression in tissue samples was determined using specific monoclonal antibodies and data were analyzed using Chi-square test and Fisher's exact test. Patient survival was analyzed after 4 years of follow-up using the Cox regression model. Results: PTEN gene expression was evident in 70 of 100 cnacer samples but was found at high levels in all non-cancer samples. There was an inverse significant relationship between PTEN gene expression and tumour stage or tumour grade (p<0.001). The expression of PTEN in invasive ductal tumours was lower than in non-invasive tumours. There was also an inverse significant relationship between the hazard of death and PTEN gene expression (p<0.001). In addition, there was an inverse significant relationship between tumour stage and hazard of death (p<0.001). Conclusion: These findings indicate that lack of PTEN gene expression can be a sign of a worse prognosis and poor survival in breast cancer cases.

ORiginal Article : Characterization and Prognostic Value of Mutations in Exons 5 and 6 of the p53 Gene in Patients with Colorectal Cancers in Central Iran

( Rahim Golmohammadi ),( Mohammad J Namazi ),( Mehdi Nikbakht ),( Mohammad Salehi ),( Mohammad H Derakhshan ) The Editorial Office of Gut and Liver 2013 Gut and Liver Vol.7 No.3

Background/Aims: We aimed to investigate the relationships among various mutations of the p53 gene and their protein products, histological characteristics, and disease prognosis of primary colorectal cancer in Isfahan, central Iran. Methods: Sixty-one patients with colorectal adenocarcinoma were enrolled in the study. Mutations of the p53 gene were detected by single-stranded conformation polymorphism and DNA sequencing. The protein stability was evaluated by immunohistochemistry. Patients were followed up to 48 months. Results: Twenty-one point mutations in exons 5 and 6 were detected in the tumor specimens of 14 patients (23%). Of those, 81% and 9.5% were missense and nonsense mutations, respectively. There were also two novel mutations in the intronic region between exons 5 and 6. In 11 mutated specimens, protein stability and protein accumulation were identified. There was a relationship between the type of mutation and protein accumulation in exons 5 and 6 of the p53 gene. The presence of the mutation was associated with an advanced stage of cancer (trend, p<0.009). Patients with mutated p53 genes had significantly lower survival rates than those with wild type p53 genes (p<0.01). Conclusions: Mutations in exons 5 and 6 of the p53 gene are common genetic alterations in colorectal adenocarcinoma in central Iran and are associated with a poor prognosis of the disease. (Gut Liver 2013; 7:295-302)

Modeling and Analyzing One Vendor-Multiple Retailers VMI SC Using Stackelberg Game Theory

Amir-Mohammad Golmohammadi,Negar Jahanbakhsh Javid,Lily Poursoltan,Hamid Esmaeeli 대한산업공학회 2016 Industrial Engineeering & Management Systems Vol.15 No.4

Game theory is a powerful tool for analyzing the Supply chain (SC) with different conflicting elements. Among them, the Stackelberg game is the one in which a player as leader has more power than the other ones as followers. Since in many SC systems one element has, in essence, more power than the others; the Stackelberg game has found many applications in SC studies. In this paper, we apply the Stackelberg game-theoretic approach and the corresponding equilibrium point to formulate and analyze a two echelon VMI SC. Comprehensive computational results on an experimental case are conducted to numerically analyze the performance of VMI system against three groups of critical parameters. Moreover, a critical comparison demonstrates the poorer performance of decentralized VMI system than centralized one. This naturally necessitates designing proper contracts between VMI partners in order to more effectively implement the realistic decentralized system.

Amino Acid Sequence Based Method for Prediction of Cell Membrane Protein Types

Seyed Koosha Golmohammadi,Lukasz Kurgan,Brendan Crowley,Marek Reformat 보안공학연구지원센터 2008 International Journal of Hybrid Information Techno Vol.1 No.1

Cell membrane proteins play a vitally important role in influencing the behavior of cells. Knowledge of membrane protein type facilitates the determination of its functions, which has implications in numerous applications including drug design. Due to the increasingly large number of uncharacterized proteins in data-banks such as NCBI’s RefSeq, there is a high desire to replace time and cost consuming experimental methods for membrane protein type classification with computational methods. This paper introduces a new computational method that accurately predicts the type of unclassified membrane proteins based on their sequence. Our method is based on a novel representation of protein sequences that incorporates seven different feature sets. Empirical comparison, which includes twelve competing methods, shows that the presented method generates predictions that result in 8% and 28% error rate reduction when compared with the best existing computational method and when using the jackknife test and testing on an independent dataset, respectively. We also show that the most influential sources of information for making the predictions include the composition of 2-gram exchange groups and the amino acid composition of the underlying sequence.

Gold Shell Nanocluster Networks in Designing Four-branch (1×4) Y-Shape Optical Power Splitters

Arash Ahmadivand,Saeed Golmohammadi 한국광학회 2014 Current Optics and Photonics Vol.18 No.3

In this study, closely spaced Au nanoparticles which are arranged in nanocluster (heptamer) configurations have been employed to design efficient plasmonic subwavelength devices to function at the telecommunication spectrum (λ~1550 nm). Utilizing two kinds of nanoparticles, the optical properties of heptamer clusters composed of Au rod and shell particles that are oriented in triphenylene molecular fashion have been investigated numerically, and the cross-sectional profiles of the scattering and absorption of the optical power have been calculated based on a finite-difference time-domain (FDTD) method. Plasmon hybridization theory has been utilized as a theoretical approach to characterize the features and properties of the adjacent and mutual heptamer clusters. Using these given nanostructures, we designed a complex four-branch (1×4) Y-shape splitter that is able to work at the near infrared region (NIR). This splitter divides and transmits the magnetic plasmon mode along the mutual heptamers arrays. Besides, as an important and crucial parameter, we studied the impact of arm spacing (offset distance) on the guiding and dividing of the magnetic plasmon resonance propagation and by calculating the ratio of transported power in both nanorod and nanoshell-based structures. Finally, we have presented the optimal structure, that is the four-branch Y-splitter based on shell heptamers which yields the power ratio of 23.9% at each branch, 4.4 μm decaying length, and 1450 nm offset distance. These results pave the way toward the use of nanoparticles clusters in molecular fashions in designing various efficient devices that are able to be efficient at NIR.

Experimental study of the turbulent convective heat transfer of titanium oxide nanofluid flowing inside helically corrugated tubes

Mohammad Reza Salimpour,Kia Golmohammadi,Ahmad Sedaghat,Antonio Campo 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.9

The convective heat transfer for the turbulent flow of water/TiO2 nanofluid inside helically horizontal corrugated tubes is investigatedin this paper using experimental techniques. The tube boundary condition is a uniform wall temperature. The test apparatus was designedand assembled with a test section containing 93 cm copper tubes with internal and external diameters of 7.71 mm and 9.52 mm, respectively. First, the heat transfer characteristics of the distilled water turbulent flow in a plain copper tube were measured preliminarily. Second,various test runs were performed for nanofluids with two nanoparticle concentrations (0.1% and 0.5%), two corrugation depth todiameter ratios (0.0648 and 0.103), two corrugation pitch to diameter ratios (0.917 and 1.297), and two corrugation width to diameterratios (0.363 and 0.492) that were all within the range of turbulent Reynolds numbers (3000 < Re < 15000). The experimental resultsreveal that the Nusselt number augments the dual increments in corrugation depth and width and with the decrements in corrugation pitch,particularly for high Reynolds numbers. The nanoparticles have a stronger effect on the heat transfer in helically corrugated tubes withhigher corrugation depths and widths as well as lower corrugation pitches. A correlation for the Nusselt number in terms of the helicallycorrugated tubes is introduced based on the linear regression analysis of the experimental data.

Field Study of Effects of Night Shifts on Cognitive Performance, Salivary Melatonin, and Sleep

Kazemi, Reza,Motamedzade, Majid,Golmohammadi, Rostam,Mokarami, Hamidreza,Hemmatjo, Rasoul,Heidarimoghadam, Rashid Occupational Safety and Health Research Institute 2018 Safety and health at work Vol.9 No.2

Background: Night shift work is associated with many problems such as sleep deprivation, sleepiness, decreased cognitive performance, increased human errors, and fatigue. This study set out to measure cognitive performance, melatonin rhythms, and sleep after different consecutive night shifts (7 vs. 4) among control room operators (CORs). Methods: The participants included 60 CORs with a mean age of 30.2 years (standard deviation, 2.0) from a petrochemical complex located in Southern Iran. Cognitive performance was assessed using the n-back task and continuous performance test. To evaluate melatonin, saliva was collected and tested by enzyme-linked immunosorbent assay. To assess sleep and sleepiness, the Pittsburgh Sleep Quality Index and Karolinska Sleepiness Scale were used, respectively. Results: Individuals who worked 7 consecutive night shifts had a significantly better cognitive performance and sleep quality than those who worked 4 consecutive night shifts. However, salivary melatonin profile and sleepiness trend were not affected by shift type. Conclusion: The main duty of CORs working night shifts at the studied industry included managing safety-critical processes through complex displays; a responsibility that demands good cognitive performance and alertness. It is suggested that an appropriate number of consecutive night shifts in a rotating shift system should be planned with the ultimate aim of improving CROs performance/alertness and enhancing safety.

Individual Fit Testing of Hearing Protection Devices Based on Microphone in Real Ear

Biabani, Azam,Aliabadi, Mohsen,Golmohammadi, Rostam,Farhadian, Maryam Occupational Safety and Health Research Institute 2017 Safety and health at work Vol.8 No.4

Background: Labeled noise reduction (NR) data presented by manufacturers are considered one of the main challenging issues for occupational experts in employing hearing protection devices (HPDs). This study aimed to determine the actual NR data of typical HPDs using the objective fit testing method with a microphone in real ear (MIRE) method. Methods: Five available commercially earmuff protectors were investigated in 30 workers exposed to reference noise source according to the standard method, ISO 11904-1. Personal attenuation rating (PAR) of the earmuffs was measured based on the MIRE method using a noise dosimeter (SVANTEK, model SV102). Results: The results showed that means of PAR of the earmuffs are from 49% to 86% of the nominal NR rating. The PAR values of earmuffs when a typical eyewear was worn differed statistically (p < 0.05). It is revealed that a typical safety eyewear can reduce the mean of the PAR value by approximately 2.5 dB. The results also showed that measurements based on the MIRE method resulted in low variability. The variability in NR values between individuals, within individuals, and within earmuffs was not the statistically significant (p > 0.05). Conclusion: This study could provide local individual fit data. Ergonomic aspects of the earmuffs and different levels of users experience and awareness can be considered the main factors affecting individual fitting compared with the laboratory condition for acquiring the labeled NR data. Based on the obtained fit testing results, the field application of MIRE can be employed for complementary studies in real workstations while workers perform their regular work duties.