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- 저자 : George K. Roderick (검색결과 4 건)
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벼멸구(Nilaparvata lugens)에서 마이크로새털라이트 마커의 분리 및 특성검정
문점희,송유한,Mun Jeomhee,Song Yoo Han,Roderick George K. Korean Society of Applied Entomology 2004 한국응용곤충학회지 Vol.43 No.4
벼멸구(Nilaparvata lugens)는 벼에 가장 큰 피해를 주는 해충 중의 하나로서, 마이토콘드리아 DNA를 분석한 선행 연구결과에 의하면 북 베트남의 홍하유역을 중심으로 남쪽과 북쪽의 개체군이 유전적으로 뚜렷한 차이를 보이고 있다. 그러나 이러한 마이토콘드리아 DNA의 변이로는 좀더 상세한 지역간 개체군의 유전적 변이를 검정할 수 없으므로, 마이크로새털라이트 마커를 이용할 수 있는 방법을 모색하였다. 총 37개 마이크로새털라이트 위치를 분석한 결과 5개 위치에서 성공적으로 라벨을 할 수 있었으며, 그 중 2개 위치에서 유용한 개체군 변이정보를 얻을 수 있었다. 이러한 두 위치에서 벼멸구의 생태형(1, 2, 3형)에 따른 변이를 검정할 수 있는지의 여부를 검정한 결과, 두 위치 중에서 한 곳(27035)에서는 생태형간의 차이를 나타내지 않았으나, 다른 한 곳(7314)에서는 생태형 간에 차이를 보였다. 따라서 마이크로새털라이트 마커를 이용하면 좀 더 상세한 벼멸구 지역 개체군의 차이를 검정하여 이동과 분산의 근원과 경로를 알아내는데 유용한 방법이 될 것으로 생각된다. The brown planthopper, Nilaparvata lugens, is among the most serious insect pests of rice. It is widely distributed in Asia, Australia and Pacific islands. An earlier mitochondrial DNA study revealed that there exist significant genetic differences between populations north and south of the Red River Delta region in Vietnam. However the mitochondrial DNA was not sufficiently variable to examine the sources of immigration. For a more detailed analysis of geographic population structure of N. lugens, we developed microsatellite markers. Thirty-seven putative microsatellite loci were isolated using a magnetic biotin method, and five primer pairs designed from the flanking regions of sequenced microsatellite clones were labeled with fluorescent. Of these five primer sets, two have proven to be useful across all the samples we used in this study. We used variation at these two microsatellite loci to test the hypothesis that N. lugens biotypes (1, 2, and 3) sampled from laboratory selection constituted distinct genetic units. Allele frequency differences among the three major biotype categories were not significantly different at one locus (27035). However, the other (7314) did show differences among the major three biotypes. The methods we describe here will be useful for studying population structure of crop pest and for tracking the patterns of migratory pest like the rice planthoppers.
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Molecular Genetic Diagnosis of Four Fruit Fly Species(Tephritidae)
Mun, Jeom-Hee,Kim, Ji-Soo,Song, Yoo-Han,Kim, Tae-Heung,George K. Roderick Korean Society of Applied Entomology 2000 Journal of Asia-Pacific Entomology Vol.3 No.2
The true fruit flies (Diptera: Tephritidae) are major agricultural pests worldwide. In Korea, imported crops are carefully scrutinized to prevent infestation by exotic tephritids and rapid species idnetification is required to make quarantine decisions. At quarantine checkpoints, tephritids are most commonly intercepted at the larval stage, however larvae have few diagnostic morphological features. Here we describe a molecular genetic method using restriction enzyme digestions of PCR products from the mitochondrial gene, cytochrome oxidase I(COI). This technique provides a simple diagnostic marker for Korean fruit flies in the genus Bacterocera(B. depressa, pumpkin fruit fly; and B. scutellata, pumpkin flower fruit fly) and wide spread fruit flies (B. dorsalis, oriental fruit fly; and Ceratitis capitata, medfly). The restriction enzymes ApaI, NheI and SacI were used to digest the 815 bp PCR product into fragments that uniquely diagnose each species. The simplicity and relatively low cost of this molecular approach will facilitate rapid quarantine decisions concerning exotic fruit flies.
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Min Zhu,Song, Yoo-Han,Uhm, Ki-Baek,Richard W. Turner,Lee, Hoon-Ho,George K. Roderick Korean Society of Applied Entomology 2000 Journal of Asia-Pacific Entomology Vol.3 No.1
Brown planthopper (nilaparvata Lugens) is a migrant pest which can migrate from tropical area into subtropical and temperate area every year and caused a lot of damage in rice production. To understand the migration process of this pest, the Boundary Layer Atmospheric(BLAYER) model and Geographic Information System(GIS) were used to analyze the migration waves from June to July in 1997, 1998 and 1999 in South Korea. The simulation results showed: 1) Each migration wave had different mass distribution and immigration area at different time; 2) The vertical air current value distributed in BPH taking-off and landing area was about several centimeters per second, which is lower than BPH flight ability. 3) The trajectory route showed that BPH had different migration routes emigrated from different source areas according to the weather system. The main source of BPH immigrated in South Korea was from the East part of Guangdong Province and South East part of Fujian Province, Perple's Republic of China. 4) Comparing with the different migration heights (733m, 1,348m and 1,963m above ground level), BPH mass distributed more northwestward in low height than in high height. 5) BPH mass movement also gave another evidence to identify the light trap data in late July.
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