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RISS 인기검색어
- 검색키워드
- 저자 : Gen-Da Chen (검색결과 4 건)
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Gold Immunolocalization of Rubisco and Rubisco Activase in Pyrenoid of Chlamydomonas reinhardtii
He, Pei-Min,Zhang, Da-Bing,Chen, Geng-Yun,Liu, Qi-Gen,Wu, Wei-Ning The Korean Society of Phycology 2003 ALGAE Vol.18 No.2
The pyrenoid ultrastructure and distribution of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and Rubisco activase in the chloroplasts of Chlamydomonas reinhardtii was studied using the immunogold localization technology with electron microscopy. There were several tubular thylakoids invading in the pyrenoid matrix to form several spokewise channels. The connections between pyrenoid matrix and stroma of chloroplast were the partial of channels. The starch sheath surrounding the pyrenoid was separated into several parts by the connections in transection. Some thylakoids were packed together near the connections in one side of the pyrenoid. Those special structures might be used to transport substance between pyrenoid and stroma of chloroplasts. With the antibody raised against the large subunits of Rubsico from C. protothecoides, the result of the gold immunolocalization of Rubisco in Chlamydomonas reinhardtii showed most of the gold particles heavily labeled the pyrenoid matrix, as well as the starch sheath matrix, and very few in the stroma of chloroplasts. The gold particle density was 880.00 $\pm$ 164.32, 190.00 $\pm$ 152.39 and 9.60 $\pm$ 5.37 ${\mu}m^{-2}$ in pyrenoid matrix, starch sheath and stroma region of chloroplast respectively (background: 5.67 $\pm$ 1.53 ${\mu}m^{-2}$). 99.59% of the total Rubiscos was calculated to be concentrated in the pyrenoid matrix and starch sheath by spatial densities. The gold immunolocalization of Rubisco activase also showed that Rubisco activase was mainly concentrated in the periphery of the pyrenoid and the starch sheath (the density was as high as 229.69 $\pm$ 96.96 ${\mu}m^{-2}$). There were very few gold particles located in the stroma of chloroplasts. These results indicated that pyrenoid surface and starch sheath was the site for Rubisco activation and $CO_2$ fixation, which supported the suggestion that pyrenoids perform photosynthesis function.
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In-situ test and dynamic response of a double-deck tied-arch bridge
Hongye Gou,Wen Zhou,Gen-Da Chen,Yi Bao,Qianhui Pu 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.27 No.2
In this study, in-situ dynamic tests of the world's longest steel box tied-arch bridge over the Yangtze River, China, are reported. The double deck bridge supports highway and monorail systems at upper and lower levels, respectively. Strain, displacement, and acceleration responses were measured and used to investigate the vibration characteristics of the bridge when excited by running trains and/or trucks at a speed of 5-60 km/h, train braking, and truck bouncing. Impact factors were correlated with the running speed of trains and trucks. A three-dimensional finite element model of the coupled monorail-train-bridge vibration system accounting for track irregularities was established to understand the system behavior and validated by the experimental results. Truck bouncing was the dominant impact factor on bridge responses. The running speed of vehicles determined the riding comfort of traveling trains.
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Damage detection of nonlinear structures with analytical mode decomposition and Hilbert transform
Zuo-Cai Wang,Dong Geng,Wei-Xin Ren,Guang-Feng Zhang,Gen-Da Chen 국제구조공학회 2015 Smart Structures and Systems, An International Jou Vol.15 No.1
This paper proposes an analytical mode decomposition (AMD) and Hilbert transform method for structural nonlinearity quantification and damage detection under earthquake loads. The measured structural response is first decomposed into several intrinsic mode functions (IMF) using the proposed AMD method. Each IMF is an amplitude modulated-frequency modulated signal with narrow frequency bandwidth. Then, the instantaneous frequencies of the decomposed IMF can be defined with Hilbert transform. However, for a nonlinear structure, the defined instantaneous frequencies from the decomposed IMF are not equal to the instantaneous frequencies of the structure itself. The theoretical derivation in this paper indicates that the instantaneous frequency of the decomposed measured response includes a slowly-varying part which represents the instantaneous frequency of the structure and rapidly-varying part for a nonlinear structure subjected to earthquake excitations. To eliminate the rapidly-varying part effects, the instantaneous frequency is integrated over time duration. Then the degree of nonlinearity index, which represents the damage severity of structure, is defined based on the integrated instantaneous frequency in this paper. A one-story hysteretic nonlinear structure with various earthquake excitations are simulated as numerical examples and the degree of nonlinearity index is obtained. Finally, the degree of nonlinearity index is estimated from the experimental data of a seven-story building under four earthquake excitations. The index values for the building subjected to a low intensity earthquake excitation, two medium intensity earthquake excitations, and a large intensity earthquake excitation are calculated as 12.8%, 23.0%, 23.2%, and 39.5%, respectively.
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Damage detection of nonlinear structures with analytical mode decomposition and Hilbert transform
Wang, Zuo-Cai,Geng, Dong,Ren, Wei-Xin,Chen, Gen-Da,Zhang, Guang-Feng Techno-Press 2015 Smart Structures and Systems, An International Jou Vol.15 No.1
This paper proposes an analytical mode decomposition (AMD) and Hilbert transform method for structural nonlinearity quantification and damage detection under earthquake loads. The measured structural response is first decomposed into several intrinsic mode functions (IMF) using the proposed AMD method. Each IMF is an amplitude modulated-frequency modulated signal with narrow frequency bandwidth. Then, the instantaneous frequencies of the decomposed IMF can be defined with Hilbert transform. However, for a nonlinear structure, the defined instantaneous frequencies from the decomposed IMF are not equal to the instantaneous frequencies of the structure itself. The theoretical derivation in this paper indicates that the instantaneous frequency of the decomposed measured response includes a slowly-varying part which represents the instantaneous frequency of the structure and rapidly-varying part for a nonlinear structure subjected to earthquake excitations. To eliminate the rapidly-varying part effects, the instantaneous frequency is integrated over time duration. Then the degree of nonlinearity index, which represents the damage severity of structure, is defined based on the integrated instantaneous frequency in this paper. A one-story hysteretic nonlinear structure with various earthquake excitations are simulated as numerical examples and the degree of nonlinearity index is obtained. Finally, the degree of nonlinearity index is estimated from the experimental data of a seven-story building under four earthquake excitations. The index values for the building subjected to a low intensity earthquake excitation, two medium intensity earthquake excitations, and a large intensity earthquake excitation are calculated as 12.8%, 23.0%, 23.2%, and 39.5%, respectively.
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