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Gündüz Gizem,Beler Merih,Ünal İsmail,Cansız Derya,Emekli-Alturfan Ebru,Kose Kemal Naci 한국독성학회 2023 Toxicological Research Vol.39 No.4
Porphyromonas gingivalis (P. gingivalis), a key pathogen in periodontal diseases, is also associated with hyperglycemiaassociated systemic diseases, including diabetes mellitus (DM). Gingipains are the most important endotoxins of P. gingivalis, and in vivo studies using gingipains are scarce. Zebrafish (Danio rerio) is a vertebrate with high physiological and genetic homology with humans that has multiple co-orthologs for human genes, including inflammation-related proteins. The aim of our study was to determine the effects of gingipain in a hyperglycemia-induced zebrafish model by evaluating inflammation, oxidant-antioxidant status, and the cholinergic system. Adult zebrafish were grouped into the control group (C), hyperglycemia-induced group subjected to 15 days of overfeeding (OF), gingipain-injected group (GP), and gingipain-injected hyperglycemic group (OF + GP). At the end of 15 days, an oral glucose tolerance test (OGTT) was performed, and fasting blood glucose (FBG) levels were measured. Lipid peroxidation (LPO), nitric oxide (NO), glutathione (GSH), glutathione S-transferase, catalase, acetylcholinesterase (AChE), alkaline phosphatase (ALP), and sialic acid (SA) levels were determined spectrophotometrically in the hepatopancreas. The expression levels of tnf-⍺, il-1β, ins, crp, and the acute phase protein YKL-40 analogs chia.5 and chia.6 were evaluated by RT‒PCR. After two weeks of overfeeding, significantly increased weight gain, FBG, and OGTT confirmed that the zebrafish were hyperglycemic. Increased oxidative stress, inflammation, and AChE and ALP activities were observed in both the overfeeding and GP groups. Amplification of inflammation and oxidative stress was evident in the OF + GP group through increased expression of crp, il-1β, chia.5, and chia.6 and increased LPO and NO levels. Our results support the role of gingipains in the increased inflammatory response in hyperglycemia-associated diseases.
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Helvacı Gizem,Uçar Aslı,Çelebi Mehmet Mesut,Çetinkaya Haydar,Gündüz Ayşe Zülal 한국영양학회 2023 Nutrition Research and Practice Vol.17 No.4
BACKGROUND/OBJECTIVES: Mediterranean diet is an environmentally friendly and healthy diet model. The diet offers many vegetables, fruits, nuts, and olive oil to consumers. In addition, it provides moderate amounts of fish and chicken, smaller quantities of dair y products, red meat, and processed meat. The Mediterranean diet has a high anti- inflammator y and antioxidant content, and it causes many physiological changes that can provide a physical performance advantage. This study examined the effects of a 15-day menu, which was planned using foods with a low acid load within the Mediterranean diet rules, on the exercise performance, lactate elimination, anthropometric measurements, and body composition. SUBJECTS/METHODS: Fifteen professional male athletes between the ages of 13 and 18, who were engaged in ski running, were included in the experimental study. Dietar y inter vention was applied for 15 days. The athlete performances were evaluated by applying the vertical jump test, hand grip strength, 20 meters shuttle run test, and Borg fatigue scale. After the shuttle run test (ever y 3 min for 30 min), blood was drawn from the finger, and the lactate elimination time was calculated. Performance and lactate measurements, body analysis, and anthropometric measurements were taken before and after dietar y inter vention. RESULTS: The vertical jump height and hand grip strength increased after the inter vention (P < 0.05). The test duration, total distance, the number of shuttles, and maximum oxygen consumption parameters of the shuttle run test increased (P < 0.05). After the inter vention, the athletes’ perceived fatigue scores decreased in several stages of the shuttle run test (P < 0.05). The lactate elimination time and athlete’s body composition were similar in repeated measurements (P > 0.05). In the last measurements, the upper middle arm circumference decreased while the height of the athletes increased (P < 0.05). CONCLUSIONS: These results show that the Mediterranean diet is a safe and feasible dietar y approach for aerobic performance and strength increase.
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Rıza Atav,Öner Gündüz,Sercan Yaz,Gizem Çakan,Bahadırhan Sevgili 한국섬유공학회 2023 Fibers and polymers Vol.24 No.8
Today, sustainable production has become an important concept. As the trends in the world bring about the widespread use of used-looking clothes, it has become mandatory to apply processes such as spray rodeo, which is the process done by spraying permanganate with a special spray gun or applying it with a brush to the desired area. However, permanganate has risk of causing irritation to the face and eyes, and cough, mucosal irritations, shortness of breath, etc. Therefore, it is of great importance to replace permanganate with clean alternatives. In this study, it was aimed to obtain high bleaching efficiency via laser-activated organic bleaching process and thus to obtain permanganate-like results in a sustainable way. It was found that the effects of organic bleaching agent concentration and pixel time on the degree of color fading are statistically significant and the optimum color fading could be achieved at 30% bleaching agent concentration and 300 μs pixel time. However, the bleaching agent concentration and/or pixel time can be changed according to the desired degree of color fading. Results indicated that by using laser-activated organic bleaching process, it is possible to obtain nearly same color fading effects with that of permanganate bleaching, which means a cleaner alternative has been developed.
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