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RISS 인기검색어
- 검색키워드
- 저자 : Filomena Nazzaro (검색결과 4 건)
- 무료
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Florinda Fratianni,Filomena Nazzaro,Aurelio Marandino,Maria del Rosario Fusco,Raffaele Coppola,Vincenzo De Feo,Laura De Martino 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.5
We studied the polyphenol composition and antioxidant activity of the ethanolic and ethyl acetate extracts of Hypericum connatum (Guttiferae), in addition to their antimicrobial and antiquorum-sensing (AQS) properties. The extracts exhibited a similar polyphenol content and a notable antioxidant activity, which was stronger in the ethanolic extract (EC50=3.2 μg/mL). Ultrahigh-performance liquid chromatography analyses revealed different polyphenol profiles, with rutin and apigen being the main components of the ethanolic extract and caffeic acid, (–)-epicatechin and p-coumaric acid being abundant in the ethyl acetate extract. The ethyl acetate extract exhibited a more effective antimicrobial activity than the ethanolic extract against the pathogens Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa. For the first time, AQS activity was detected in H. connatum: the ethanolic extract inhibited the production of violacein, a quorum-sensing–regulated pigment, in a Chromobacterium violaceum tester strain, without interfering with its growth.
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Florinda Fratianni,Raffaele Coppola,Filomena Nazzaro 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.9
This study investigated the phenolic composition and antioxidant capacity of an ethanolic extract from the peel of the Annurca (Malus domestica var. Annurca), which is widespread in the Campania region of southern Italy. The antimicrobial effect of the extract on different pathogens was also examined. The potential antiquorum sensing activity of the extract was tested by using the microorganism Chromobacterium violaceum. Ultra-performance liquid chromatography revealed that rutin, epicatechin, dicaffeoylquinic acid, and caffeic acid were the most abundant phenolic compounds in the extract; these compounds constituted 27.43%, 24.93%, 16.14%, and 15.3% of the total phenols, respectively. The test for 2,2-diphenyl-1-picryl-hydrazyl free radical–scavenging activity showed that the extract possessed an impressive antioxidant capacity (50% effective concentration of 2.50 μg/g of product). Furthermore, the extract clearly exhibited antimicrobial activity against Bacillus cereus (11- to 14-mm diameter of inhibition halo, depending on the strain) and Escherichia coli serotype O157:H7 (10-mm diameter of inhibition halo). No activity was observed against the probiotic lactobacilli tested or against Staphylococcus aureus. Antiquorum sensing activity was reported for the first time for apple extracts. In conclusion, these results indicate the potential of this extract for treating some microbial infections through cell growth inhibition or quorum sensing antagonism, thereby validating the health benefits of apples.
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Biochemical Characteristics and Biological Properties of Annurca Apple Cider
Florinda Fratianni,Alfonso De Giulio,Alfonso Sada,Filomena Nazzaro 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.1
Our work aimed to investigate the phenolic composition and antioxidant capacity of the de-alcoholized extract of cider obtained from the Annurca apple (Malus domestica var. Annurca). The antimicrobial effect of the extract against different pathogens, including Chronobacter sakazakii, was also examined. The extract’s potential anti-quorum-sensing (AQS) activity was assessed using the test microorganism Chromobacterium violaceum. Biochemical analysis of the extract using ultra-performance liquid chromatography revealed catechin and caffeic acid as the most abundant polyphenols present,which represented about 35.5% and 36.6% of the total phenolics identified, respectively. An antioxidant capacity was also found (50% effective concentraiton = 10 lL). The extract exhibited clear antimicrobial activity against all strains used in the experiments. Escherichia coli and Bacillus cereus were the most sensitive bacteria to the antimicrobial activity. The extract also inhibited the growth of the emergent pathogen strain C. sakazakii. The AQS activity of apple cider is reported here for the first time. In conclusion, our results demonstrate some biological properties of the apple cider and contribute to reinforcing the potential of the apple and its derivatives as functional components of the diet.
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Florinda Fratianni,Riccardo Riccardi,Patrizia Spigno,Maria Neve Ombra,Autilia Cozzolino,Patrizio Tremonte,Raffaele Coppola,Filomena Nazzaro 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.7
Extracts of the bulbs of the two endemic varieties “Rosato” and “Caposele” of Allium sativum of the Campania region, Southern Italy, were analyzed. The phenolic content, ascorbic acid, allicin content, and in vitro antimicrobial and antifungal activity were determined. Ultra performance liquid chromatography with diode array detector performed polyphenol profile. The polyphenolic extracts showed antioxidant activity (EC50) lower than 120 mg. The amount of ascorbic acid and allicin in the two extracts was similar. Polyphenol extract exhibited antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and (only by the extract of Rosato) against Bacillus cereus. The extract of Caposele was more effective in inhibiting the growth of Aspergillus versicolor and Penicillum citrinum. On the other hand, the extract of Rosato was effective against Penicillium expansum.
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