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박성빈,Kyoung Kwon,차인석,장호빈,노성원,Fernand F. Fagutao,Young Kyu Kim,류종언,정태성 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.1
A multiplex PCR protocol was established tosimultaneously detect major bacterial pathogens in oliveflounder (Paralichthys olivaceus) including Edwardsiella(E.) tarda, Streptococcus (S.) parauberis, and S. iniae. ThePCR assay was able to detect 0.01 ng of E. tarda, 0.1 ng of S. parauberis, and 1 ng of S. iniae genomic DNA. Furthermore,this technique was found to have high specificity when testedwith related bacterial species. This method represents acheaper, faster, and reliable alternative for identifyingmajor bacterial pathogens in olive flounder, the mostimportant farmed fish in Korea.