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Wang Jie,Wang Shuangyu,Sun Fenglin,Liu Chang,Zhao Jinquan,Yu Hongwei,Lv Xiaojing,Liu Ze,Bu Shuhua,Yu Weisen 한국식물생명공학회 2024 Plant biotechnology reports Vol.18 No.2
Some edible Leguminous are toxic when raw, and the Chinese are particularly fond of beans, so Leguminous poisoning is very common in China. Rapid and accurate identification of poisoned species and determination of their toxic components would better assist physicians in treating patients. However, traditional morphology-based identification methods possess many limitations. DNA barcoding technique is a new species identification technique developed in recent years, which is expected to make up for the shortcomings of traditional morphological identification. In this study, a comprehensive evaluation system based on DNA barcoding and ELISA kits was attempted. A total of 30 Leguminous toxic plants were collected, involving 9 genera and 10 species. We used simulated gastric fluid (SGF) to simulate the human gastric environment. Three markers (rbcL, trnH-psbA, and ITS) were amplified and sequenced for all untreated and 15 mock-digested samples. The validity of DNA barcoding for species identification was assessed using the Basic Local Alignment Search Tool (BLAST) method and the tree construction method. The levels of three toxic components (saponin, phytoagglutin and trasylol) were determined in all samples using ELISA kits. The amplification success rate of all three regions was high (rbcL 96.67%, trnH-psbA 100%, and ITS 100%), but the sequencing of the trnH-psbA region was less satisfactory (66.67%), and SGF had a significant impact on the sequencing of the ITS region (After 40 min of SGF treatment, the sequencing success rate decreased by 46.67%). The samples from different species and origins contained different levels of toxic components, and the levels of all three sub- stances decreased significantly after undergoing SGF digestion. After 1 h of SGF treatment, the saponin content decreased to 0–8.60% in untreated content (PHA decreased to 8.62–36.88%, trasylol decreased to 4.70–47.06%). The current results suggest that DNA barcoding has great potential for rapid identification of Leguminous poisoning in clinical settings. Toxins are probably not detectable in the patient for longer periods of poisoning. We recommend DNA barcoding technology as a first step for rapid screening and combined with toxin analysis for clinical diagnosis.
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Yanjun Zhao,Chenglin Liu,Hua Zhang,Licheng Wang,Baokun Liu,Fenglin Lv 한국지질과학협의회 2015 Geosciences Journal Vol.19 No.1
The Shulu Sag is a typical rift valley basin in easternChina, where the formation mechanism of Cenozoic salt-bearingseries has been poorly understood. In this paper, petrologic analysis,X-ray powder Diffraction (XRD) analysis and the analysis ofhalite ion concentrations of the first member of the Shahejie Formationfrom two drilling cores were conducted to interpret theformation mechanism of these salt-bearing series in Shulu Sag. Theresults indicated that the salt-bearing series is mainly composed of calcilutiteor mudstone at top and bottom, and anhydrite-bearing haliteand thin interbeds of salt-bearing calcilutite and dark grey shale inthe middle part. These thin interbeds with porphyritic or petalshapedglauberite crystals or some thinner dark grey shale interbedsseparated halites into several members with the average thicknessof only 9~12 cm in the two drilling holes. This typical rhythmicpattern due to frequent alternation of concentration was evidencedby the variation of chemical composition and hydrogen isotopes ofhalite fluid inclusions. The presence of large number of primaryfluid inclusions within chevron crystals and vertically orientedbottom-growth crystals showed that the halite were precipitated inshallow water (<60 cm). These features are direct evidence for thisfrequent alternation of dissolution and concentration of brines andterrigenous detrital-chemical shallow-water sedimentary model ofthese small rift-valley basins in eastern China.
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