Mapping Quantitative Trait Loci for Awnness and Yield Component Traits in Isogenic Lines Derived from an Oryza sativa / O. rufipogon Cross

Jin, Feng-Xue,Kim, Dong-Min,Ju, Hong-Guang,Ahn, Sang-Nag 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.1

An advanced backcross line, HR9118, was produced from a single plant of $BC_{2}F_{3}$ families derived from a cross between Oryza rufipogon Griff. (IRGC 105491) as a donor parent and the O. sativa subsp. japonica cv. Hwaseongbyeo as a recurrent parent. Although HR9118 resembled Hwaseongbyeo, several traits were different from those of Hwasoengbyeo, including days to heading, plant height, and awn. These differences between Hwasongbyeo and HR9ll8 could be attributed to introgressed O. rufipogon chromosome segments into HR9118. Introgression analysis using 460 SSR markers revealed that three O. rufipogon-specific chromosome segments were detected in HR9118 genome. $F_{2:3}$ populations derived from the cross between Hwaseongbyo and HR9118, consisting of $340\;F_2$ plants and $137\;F_3$ lines, were used to map and characterize QTLs for 12 traits. QTL analysis identified a total of 17 QTLs in the $F_{2:3}$ populations. Of these, seven QTLs were shared by the $F_2$ and $F_3$, populations, whereas the other ten QTLs were identified only in the $F_3$ population. In seven (41.2%) QTLs identified in this study, the O. rufipogon-derived alleles contributed desirable agronomic effects despite the overall undesirable characteristics of the wild phenotype. Each of three O. rufipogon introgressed segments contained multiple QTLs, indicating linkage and/or pleotropic effects. A cluster of eight QTLs was detected on chromosome 8 including a major QTL for awn. Substitution mapping using F, population indicated that awn8 was located within an interval between two SSR makers RM23326 and RM23356 which are 590 kb apart. SSR markers tightly linked to QTLs for yield components detected in this study will facilitate cloning of the gene underlying this QTL as well as marker-assisted selection for variation in grain weight in an applied breeding program.

Detection of epistatic interaction of two QTLs, <i>gw8.1</i> and <i>gw9.1</i>, underlying grain weight using nearly isogenic lines in rice

Jin, Feng-Xue,Ji, Shi-Dong,Xie, Xiao-Bo,Kang, Ju-Won,Ju, Hong-Guang,Ahn, Sang-Nag Japanese Society of Breeding 2011 Breeding science Vol.61 No.1

<P>Grain weight (GW) is one of the most important targets for grain yield in rice breeding. In previous studies, two quantitative trait loci (QTLs) for GW, <I>gw8.1</I> and <I>gw9.1</I>, have been identified using progeny derived from a cross between the <I>japonica</I> cultivar Hwaseong and <I>Oryza rufipogon </I>(IRGC 105491). To test whether these quantitative trait loci (QTLs) have an epistatic interaction, we developed an F<SUB>2</SUB> population by crossing two nearly isogenic lines (NILs) harboring <I>gw8.1</I> and <I>gw9.1</I>. Simple sequence repeat (SSR) markers tightly linked to the QTLs were used to select F<SUB>3</SUB> QTL-NILs from the F<SUB>2</SUB> population. A two-way ANOVA revealed an epistatic interaction between the two QTLs in the F<SUB>2</SUB> population (<I>P</I> = 0.0084). This interaction was confirmed by an analysis of F<SUB>3</SUB> QTL-NILs indicating that both QTLs are involved in the same genetic mechanism controlling GW. The <I>gw8.1</I> QTL was further mapped between two SSR markers, RM23204 and RM23211, which are 110.1 kb apart. To our knowledge, this is the first report using QTL-NILs to reveal an epistatic interaction between QTLs for GW.</P>

Pyramiding of 2 QTLs, gw8 and gw9, underlying grain weight using nearly isogenic Lines (NILs) in rice

Feng Xue Jin,Xiao Bo Xie,Shi Dong Ji,Hong Guang Ju,Jung Pil Suh,Hung Goo Hwang,Sang Nag Ahn 한국육종학회 2008 한국육종학회 학술발표회 발표요지 Vol.40 No.1

Mapping Quantitative Trait Loci for Grain Traits Using Near Isogenic Line from a Cross between Oryza minuta and O. sativa

Feng Xue Jin,Le Hung Linh,Kyung Ho Kang,Sang Nag Ahn 한국육종학회 2005 한국육종학회지 Vol.37 No.4

Mapping Quantitative Trait Loci for Grain Traits Using Near Isogenic Line from a Cross between Oryza minuta and O. sativa

Feng-Xue Jin,Lehung Linh,Kyung-Ho Kang,Sang-Nag Ahn 한국육종학회 2005 한국육종학회지 Vol.37 No.5

Introgression has been achieved from wild species Oryza minuta (2n=48, BBCC, Acc. No. 101141) into O. sativa subsp. japonica cv. Hwaseongbyeo. Hwaseongbyeo was used as a recurrent parent and O. minuta as a donor parent. An advanced backcross line, WH79006 produced from a single plant from BC5F3 families, resembled Hwaseongbyeo, but it showed differences from Hwaseongbyeo in grain traits including 1,000 seed weight (TSW). These differences between Hwaseongbyeo and WH79006 could be attributed to the introgression of O. minuta chromosome segments into WH79006. To identify and characterize the effects of alien gene(s) introgressed into WH79006, the F2 and F3 populations from the cross between Hwaseongbyeo and WH79006 were developed. The 75 F2 plants were evaluated for 8 traits in 2003 and their progeny, the 75 F3 lines for 12 agronomic traits in 2004. Genotypes were determined for 75 F2 plants using simple sequence repeat (SSR) markers. QTL analysis was carried out to determine the relationship between marker genotype and the traits evaluated. A total of nineteen QTLs were identified using single point analysis. Among these, 4 QTLs were detected on chromosomes 7, 10 and 11 in the F2 population, which explained ranging from 5.7 to 12.6% of the total phenotype variation. Fifteen QTLs were detected on chromosomes 3, 5, 7, 8, 10 and 11 in the F3 population, which explained 4.3 to 13.5% of the phenotype variation. The number of QTLs detected per chromosome ranged from 1 to 4 in both populations. Comparison of QTLs indicated that four QTLs associated with days to heading (dth), 1,000 seed weight (tsw), seed length (sl) and L/W ratio of seed (lw) were shared in both populations and the remaining 11 only in the F3 population. Among these QTLs identified, nine QTLs have not been detected in previous QTL studies between Oryza cultivars, indicating potentially novel alleles from O. minuta. The QTLs detected in this study could be a rich source of natural genetic variation underlying the evolution and breeding of rice.

Mapping Quantitative Trait Loci for Awnness and Yield Component Traits in Isogenic Lines Derived from an Oryza sativa/O. rufipogon Cross

Feng-Xue Jin,Dong-Min Kim,Hong-Guang Ju,안상낙 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.1

An advanced backcross line, HR9118, was produced from a single plant of BC3F3 families derived from a cross between Oryzarufipogon Griff. (IRGC 105491) as a donor parent and the O. sativa subsp. japonica cv. Hwaseongbyeo as a recurrent parent. Although HR9118 resembled Hwaseongbyeo, several traits were different from those of Hwasoengbyeo, including days to heading,plant height, and awn. These differences between Hwasongbyeo and HR9118 could be attributed to introgressed O. rufipogon chromosomesegments into HR9118. Introgression analysis using 460 SSR markers revealed that three O. rufipogon-specific chromosomesegments were detected in HR9118 genome. F2:3 populations derived from the cross between Hwaseongbyo and HR9118, consistingof 340 F2 plants and 137 F3 lines, were used to map and characterize QTLs for 12 traits. QTL analysis identified a total of 17QTLs in the F2:3 populations. Of these, seven QTLs were shared by the F2 and F3 populations, whereas the other ten QTLs were identifiedonly in the F3 population. In seven (41.2%) QTLs identified in this study, the O. rufipogon-derived alleles contributed desirableagronomic effects despite the overall undesirable characteristics of the wild phenotype. Each of three O. rufipogon introgressed segmentscontained multiple QTLs, indicating linkage and/or pleotropic effects. A cluster of eight QTLs was detected on chromosome 8including a major QTL for awn. Substitution mapping using F2 population indicated that awn8 was located within an intervalbetween two SSR makers RM23326 and RM23356 which are 590 kb apart. SSR markers tightly linked to QTLs for yield componentsdetected in this study will facilitate cloning of the gene underlying this QTL as well as marker-assisted selection for variationin grain weight in an applied breeding program.

Pyramiding of 2 QTLs, gw8 and gw9, underlying grain weight using nearly isogenic lines (NILs) in rice

Feng-Xue Jin,Xiao-Bo Xie,Shi-Dong Ji,Hong-Guang Ju,Jung-Pil Suh,Hung-Goo Hwang,Sang-Nag Ahn 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10

Grain weight (GW) or grain size is the most important target not only as a major component of grain yield, but also associated with the cooking quality in rice breeding program. In a previous study, two loci for grain weight, gw8 and gw9, have been fine map to about 306.4kb and 37.4kb, respectively, in backcross populations derived from a cross between the Korean japonica cultivar Hwaseongbyeo and Oryza rufipigon (IRGC 105491). Both O.rufipogon alleles increased GW in the Hwaseongbyeo background despite the fact that O. rufipogon was the small-seeded parent. To test the effect of pyramiding the QTLs for grain weight, an F2 population consisted of 226 individuals was developed derived from the cross between the two QTLs (gw8 and gw9), and used MAS to select nearly isgenic lines for two QTLs and a pyramiding line. Molecular genotyping of F2 population revealed the existence of interaction between gw8 and gw9. Moreover, the interaction was also confirmed by phenotypic analysis of four QTL-NILs. The results suggest that epistasis interaction is important genetic basis in determining yield traits in rice.

Kinetics of a Cloned Special Ginsenosidase Hydrolyzing 3-O-Glucoside of Multi-Protopanaxadiol-Type Ginsenosides, Named Ginsenosidase Type 3

( Xue Feng Jin ),( Hong Shan Yu ),( Dong Ming Wang ),( Ting Qiang Liu ),( Chun Ying Liu ),( Dong Shan An ),( Wan Taek Im ),( Song Gun Kim ),( Feng Xie Jin ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.3

In this paper, the kinetics of a cloned special glucosidase, named ginsenosidase type III hydrolyzing 3-O-glucoside of multi-protopanaxadiol (PPD)-type ginsenosides, were investigated. The gene (bgpA) encoding this enzyme was cloned from a Terrabacter ginsenosidimutans strain and then expressed in E. coli cells. Ginsenosidase type III was able to hydrolyze 3-O-glucoside of multi-PPD-type ginsenosides. For instance, it was able to hydrolyze the 3- O-β-D-(1→2)-glucopyranosyl of Rb1 to gypenoside XVII, and then to further hydrolyze the 3-O-β-D-glucopyranosyl of gypenoside XVII to gypenoside LXXV. Similarly, the enzyme could hydrolyze the glucopyranosyls linked to the 3-O- position of Rb2, Rc, Rd, Rb3, and Rg3. With a larger enzyme reaction Km value, there was a slower enzyme reaction speed; and the larger the enzyme reaction Vmax value, the faster the enzyme reaction speed was. The Km values from small to large were 3.85 mM for Rc, 4.08 mM for Rb1, 8.85 mM for Rb3, 9.09 mM for Rb2, 9.70 mM for Rg3(S), 11.4 mM for Rd and 12.9 mM for F2; and Vmax value from large to small was 23.2 mM/h for Rc, 16.6 mM/h for Rb1, 14.6 mM/h for Rb3, 14.3 mM/h for Rb2, 1.81mM/h for Rg3(S), 1.40 mM/h for Rd, and 0.41 mM/h for F2. According to the Vmax and Km values of the ginsenosidase type III, the hydrolysis speed of these substrates by the enzyme was Rc>Rb1>Rb3>Rb2>Rg3(S)>Rd>F2 in order.

S100A4 Expression is Closely Linked to Genesis and Progression of Glioma by Regulating Proliferation, Apoptosis, Migration and Invasion

Jin, Ting,Zhang, Zhuo,Yang, Xue-Feng,Luo, Jun-Sheng Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.7

Background: The calcium-binding S100A4 protein is involved in epithelial to mesenchymal transition, oncogenic transformation, angiogenesis, cytoskeletal integrity, mobility and metastasis of cancer cells. This study aimed to clarify the roles of S100A4 in genesis and progression of glioma. Materials and Methods: S100A4 expression was examined by real-time RT-CPR and Western blot in glioma and paired normal brain tissue (n=69), and compared with clinicopathological parameters of tumors. In addition, glioma U251 cells transfected with an S100A4-expressing plasmid were examined for proliferation by MTT, apoptosis by Annexin V-FITC, and migration and invasion with Transwell chambers. Results: Increased S100A4 mRNA expression was found in gliomas, compared with paired non-tumor tissue (p<0.001). Gradual elevation of overexpression of S100A4 was observed with increasing glioma grade (p<0.001). Astrocytoma showed lower S100A4 mRNA expression than oligodendrogliomas, with glioblastomas having highest values (p<0.001). Similar results were obtained for S100A4 protein, a positive link being found between mRNA and protein expression in gliomas (p<0.001). There was higher growth, lower apoptosis, stronger migration and invasion of S100A4 transfectants than control and mock transfected cells (p<0.001). Conclusions: These findings indicate that up-regulated S100A4 expression is positively linked to pathogenesis, progression and histogenesis of glioma by modulating proliferation, apoptosis, migration and invasion.

Two-stage Gradient-based Iterative Estimation Methods for Controlled Autoregressive Systems Using the Measurement Data

Feng Ding,Lei Lv,Jian Pan,Xiangkui Wan,Xue-Bo Jin 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.4

This paper considers the parameter identification problems of controlled autoregressive systems using observation information. According to the hierarchical identification principle, we decompose the controlled autoregressive system into two subsystems by introducing two fictitious output variables. Then a two-stage gradientbased iterative algorithm is proposed by means of the iterative technique. In order to improve the performance of the tracking the time-varying parameters, we derive a two-stage multi-innovation gradient-based iterative algorithm based on the multi-innovation identification theory. Finally, an example is provided to illustrate the effectiveness of the proposed algorithms.