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Televised Political interviews: A Paradigm for Analysis
Feldman, Ofer Center for Asian Public Opinion ResearchCollaborat 2016 Asian journal for public opinion research Vol.3 No.2
This paper proposes a paradigm for research on broadcast political interviews. Developed using a series of studies, the paradigm introduces core aspects and questions that should be addressed when examining political interviews in a particular society. This paradigm will enable researchers to collect detailed accounts of the interaction between interviewers and interviewees, the structure and tone of the questions posed to interviewees and the interviewees' communicative style when addressing such questions, and the strategies employed by the participants to pursue their goals. It is hoped that the paradigm will encourage the interest and facilitate the study of televised political interviews particularly from the nationally, culturally, and socially diversified Asian countries. Gathering detailed data from non-Western societies in Asia will also facilitate our understanding of the function and effect of political communication from a cross-cultural perspective.
In Vitro Real-Time Interactions of Cranberry Constituents with Immobilized Fructosyltransferase
Mark Feldman,Ervin I. Weiss,Itzhak Ofek,Moshe Shemesh,Doron Steinberg 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.5
Cranberry has been proposed as an anti-biofilm agent that does not kill bacteria, but rather prevents the pathogen from survival in the host. This can be achieved by inhibiting the function of virulent factors essential for the pathogen to persist in a host environment. The oral bacterial enzyme fructosyltransferase (FTF) plays an important role in the pathogenesis of dental diseases. The real-time interaction of cranberry nondialyzable material (NDM) with immobilized FTF was investigated using the surface plasmon resonance (SPR) technique. To determine its binding efficiency, NDM at concentrations between 0μg/mL and 200μg/mL was applied onto the immobilized FTF. The effect of NDM or other polyphenols, myricetin, and epicatechin on FTF enzymatic activity was evaluated by applying the above compounds and sucrose onto immobilized FTF. Salivary amylase was applied with NDM onto immobilized FTF to explore the effect on NDM–FTF interaction. Our results show that NDM firmly attaches to immobilized FTF in a dose-dependent manner, whereas the presence of salivary amylase reduced this binding interaction. Using nonlinear regression we calculated that the affinity constant of NDM applied alone (106 M−1) was fivefold higher than NDM in the presence of amylase (0.2×106 M−1). At 200μg/mL, NDM, introduced together with sucrose, inhibited the activity of immobilized FTF by 63% within minutes, in comparison with the control (sucrose alone). The effect of NDM was sustained even after it was washed off the immobilized FTF. Myricetin also strongly inhibited FTF activity, whereas epicatechin was less effective. The real-time SPR observation suggests that one of the anti-biofilm modes of action of NDM is an immediate and irreversible inhibitory effect on the activity of immobilized FTF, which is due to a strong binding affinity to the immobilized enzyme