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- 저자 : Fangbiao Zhang (검색결과 1 건)
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Evaluation of an Immune-privileged Scaffold for In vivo Implantation of Tissue-engineered Trachea
Shu Pan,Fei Sun,Hongcan Shi,Fangbiao Zhang,Xingchen Liu,Weidong Zhang 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.5
Forty tracheas were harvested from donor NewZealand rabbits. Thirty of the tracheas were randomlydivided into four treatment groups corresponding to 4, 5, 6,or 7% NaClO4 and one untreated group (n = 6 each group). Scanning electron microscopy distinctly revealed thecilium of epithelial cells in the fresh trachea. The internalsurface of the trachea was rough in the 4% treatment groupand smooth in the 5% treatment group, whereas the matrixwas fractured in the 6% treatment group and highlyfractured in the 7% treatment group. We observed that thenumber of nuclei in the cells of the 4, 5, 6, and 7%treatment groups decreased compared to the cells of theuntreated group (p < 0.05). Although there was a significantdecrease in maximum tensile strength, tensile strain atfracture and the elastic modulus (p < 0.05) with increasingconcentrations of NaClO4, the content of glycosaminoglycans(GAGs) did not significantly decline (p > 0.05) in the 5%treatment group. In addition, histopathological analysisshowed that the fiber component and basement membraneof the matrix in the 5% treatment group were retained afteroptimal decellularization. Despite the preserved cartilage,in vitro immunohistochemical analysis revealed that thematrix did not show the presence of major histocompatibilitycomplex (MHC) antigens. The remaining ten donor tracheas,which were divided into a positive control group and anoptimal decellularized group, were used for allogeneictransplantation. Blood samples were taken regularly, andhistologic examinations were performed at 30 days postimplantation,which showed no significant immune rejection. In conclusion, we surveyed the structural integrity throughmorphological observation and compared the biomechanicaland immunogenic changes in the tracheal matrix under thedifferent treatments. The optimal decellularized trachealmatrix with preserved cartilage, which was acquired via 5%NaClO4 treatment, exhibited structural integrity, antigen cellremoval and immune privilege and would be suitable for useas a tissue-engineered trachea for in vivo transplantation inrabbit models.
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