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Mark E. Fabreag,Dong-Min Kim,Ju-Won Kang,Soo-Jin Kwon,Yeo-Tae Yun,Sang-Nag Ahn 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
While a wealth of genetic diversity can be found from traditional rice varieties, wild rice species and wild relatives of rice, transfer of useful genes to modern varieties are often hampered by linkage drag. In this study, the previously identified blast resistance locus Pi45(t) from a cross between ‘Ilpumbyeo’ and ‘Moroberekan’ was showed to be linked with the spreading-type panicle caused by the SPR3 locus. Using InDel4 and RM17579 linked to the Pi45(t) and the SPR3, respectively, the distance between the two loci was estimated to be 6.9cM. This suggests a tight, yet incomplete linkage and provides the opportunity to utilize Pi45(t) in breeding programs without including SPR3. Two groups based on the genotype at the SPR3 locus were assembled; the CLosed Panicle (CLP) and SPReading panicle (SPR) groups with lines, which were homozygous for the Ilpumbyeo and Moroberekan alleles, respectively. A comparison between the traits of CLP and SPR groups revealed a decrease in 1000-grain weight and length and an increase in spikelets per panicle and secondary branches in the SPR group. This complicates selection against SPR3 as it is not clear whether these quantitative trait loci are linked to either SPR3 or Pi45(t). Re-evaluation of these traits using lines recombinant at the two loci would be necessary to clarify this issue.
( Mark Edward Fabreag ),( Dong Min Kim ),( Ju Won Kang ),( Soo Jin Kwon ),( Yeo Tae Yun ),( Sang Nag Ahn ) 한국육종학회 2014 Plant Breeding and Biotechnology Vol.2 No.2
Although traditional rice varieties and wild rice species exhibit vast genetic diversity, the transfer of useful genes to modern varieties is often hampered by linkage drag. In this study, the previously identified blast resistance locus Pi45(t) from a cross between ‘Ilpumbyeo’ and ‘Moroberekan’ was linked to the spreading-type panicle caused by the SPR3 locus. Using InDel4 and RM17579 linked to the Pi45(t) and the SPR3, respectively, the distance between the two loci was estimated to be 6.9cM. This suggests a tight, yet incomplete linkage and provides the opportunity to utilize Pi45(t) in breeding programs without including SPR3. Two groups based on the genotype at the SPR3 locus were assembled; the CLosed Panicle (CLP) and SPReading panicle (SPR) groups, with lines which were homozygous for the Ilpumbyeo and Moroberekan alleles, respectively. A comparison between the traits of CLP and SPR groups revealed a decrease in 1000-grain weight and length and an increase in spikelets per panicle and secondary branches in the SPR group. This complicates selection against SPR3 as it is not clear whether these quantitative trait loci are linked to either SPR3 or Pi45(t). Re-evaluation of these traits using lines recombinant at the two loci would be necessary to clarify this issue.
High-density mapping of quantitative trait loci for grain-weight and spikelet number in rice
Kim, Dong-Min,Lee, Hyun-Sook,Kwon, Soo-Jin,Fabreag, Mark Edward,Kang, Ju-Won,Yun, Yeo-Tae,Chung, Chong-Tae,Ahn, Sang-Nag Springer New York 2014 Rice Vol.7 No.-
<P><B>Background</B></P><P>High grain yield is one of the most important traits requiring improvement in rice breeding programs. Consequently, the genetic basis of spikelets per panicle (SPP) and grain weight (TGW) have received much research focus because of their importance in rice yield.</P><P><B>Results</B></P><P>In this study, IL28, which is a near isogenic line (NIL) developed by introgressing chromosomal segments of the cultivar ‘Moroberekan’ into the cultivar ‘Ilpumbyeo’, showed a significant increase in the number of spikelets per panicle (SPP) and 1,000-grain weight (TGW) compared to the recurrent parent, Ilpumbyeo. Quantitative trait locus (QTL) analysis in 243 F2 plants derived from a cross between IL28 and Ilpumbyeo indicated that both <I>qSPP6</I> and <I>qTGW6</I> are located in the interval RM3430–RM20580. Following substitution mapping with 50 F<SUB>3:4:5</SUB> lines, <I>qSPP6</I> was mapped to a 429-kb interval between RM20521 and InDel-1, while <I>qTGW6</I> was mapped to a 37.85-kb interval between InDel-1 and SNP--3 based on the <I>japonica</I> genome sequence. This result indicates that <I>qSPP6</I> and <I>qTGW6</I> are different genes. Yield trials with substitution lines indicated that lines harboring the homozygous Moroberekan segment at both the <I>qSPP6</I> and <I>qTGW6</I> region showed significantly higher grain yield than Ilpumbyeo.</P><P><B>Conclusion</B></P><P>Because the Moroberekan alleles for SPP and TGW have been shown to be beneficial in the genetic background of Ilpumbyeo, both the <I>qSPP6</I> and <I>qTGW6</I> alleles might prove valuable in improving rice yields. Closely linked SSR markers are expected to facilitate the cloning of genes that underlie these QTLs, as well as with marker-assisted selection for variation in SPP and TGW in rice breeding programs.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/s12284-014-0014-5) contains supplementary material, which is available to authorized users.</P>