http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Suhkneung Pyo,Eunwha Son 한국식품영양과학회 2006 Preventive Nutrition and Food Science Vol.11 No.4
Recently, Rhei Rhizoma extracts (RRE) have begun to receive more attention as potential biological response modifiers. In the present study, we studied the antiproliferative effect of RRE on tumor cells and the effect of RRE on macrophage function. A variety of tumor cells and macrophages were treated with RRE at various concentrations. The effect of RRE on cell proliferation was measured by MTT assay and the effect of RRE on the production of nitric oxide (NO) was determined in the macrophage-like cell lines Raw264.7, C6 and peritoneal macrophages (pMQ). RRE inhibited the growth of tumor cells (e.g., B16, HOS). However, the effects of RRE on the production of NO varied with macrophage types. RRE had no effect on C6 cell growth and slightly increased the growth of Raw264.7 cells. In addition, treatment of normal pMQ with RRE enhanced NO production in a concentration-dependent manner, whereas RRE suppressed NO production at 50 ㎍/mL in both Raw264.7 and C6 cells. However, RRE suppressed NO production in LPS/IFN-γ-stimulated C6 cells. Overall, these results suggest that RRE elicits an antiproliferative property and differentially modulates NO production in various macrophages, and have a potential for therapeutic application.
Immunomodulatory Effects of Cimicifugae Rhizoma Extracts in Macrophages
Suhkneung Pyo,Byung-Oh Kim,Hye-Sook Choi,Eunwha Son 한국식품영양과학회 2006 Preventive Nutrition and Food Science Vol.11 No.4
Cimicifugae Rhizoma (CR) belongs to the Ranunculaceae family, which has been traditionally used to treat climacteric complaints, antipyretics and diaphoresis as an alternative medicine for estrogen hormone replacement therapy with estrogens. Recently, it has been reported that different extract fractions of CR have various effects such as anti-allergic, anti-inflammatory and anti-proliferative activities. The current study investigated the immunomodulatory effects of Cimicifugae Rhizoma water extracts (CRE) in the macrophage-like cell line, Raw 264.7. Our results showed that CRE (1~50 ㎍/mL) stimulated tumoricidal activity and NO production, whereas phagocytic activity was inhibited at the same concentrations. Additionally, iNOS mRNA expression was significantly increased in Raw 264.7 exposed to CRE as demonstrated by RT-PCR. These results indicate that the tumoricidal activity induced by CRE may be mediated by the production of NO and these activities may be useful for the treatment of diseases such as cancer.
The Effects of Boron on the Proliferation of Osteoblastic and Neuroblastoma Cells
Hye-Sook Choi,Do Hang,Mi-Kyeong Choi,Sung-Ryul Lee,Suhkneung Pyo,Eunwha Son,Mi-Hyun Kim 한국식품영양과학회 2005 Preventive Nutrition and Food Science Vol.10 No.4
It has been recently reported that boron affects bone metabolism in humans and animals. In this study we examined whether boron affects the proliferation on various cell types, MG-63, HOS, Raw 264.7 and SK-N-SH. When treated with different concentrations of boron (1, 10, 100 μM) for 24 and 48 hr, the proliferation of MG-63 cells was enhanced at 10 μM (p<0.05), for 24 hr. In HOS cells, boron had no effect on cell proliferation at 24 or 48 hr. In addition, treatment of pre-osteoclastic cells (Raw 264.7) with 1, 10, 100 μM boron resulted in no effect on cell proliferation. Proliferation of neuronal cells (SK-N-SH) was enhanced by boron in a concentration dependent manner at low concentrations (0.1, 0.5, 1 μM). Besides proliferation activity, boron has an effect on the enhancement of NO production in SK-N-SH cells in a concentration-dependent manner. These studies showed that boron enhances proliferation of osteoblastic cells (especially MG-63), depending upon the concentration of boron. These results also provide further evidence of the positive effects of boron in neuronal disease.
Do Hang,Hye-Sook Choi,Se-Chan Kang,Kyung-Ran Kim,Eun-Soo Sohn,Mi-Hyun Kim,Suhkneung Pyo,Eunwha Son 한국식품영양과학회 2005 Preventive Nutrition and Food Science Vol.10 No.4
Fucoidans, high-molecular-weight sulfated polysaccharides extracted from brown seaweeds, have various biological activities. Here we examined whether fucoidan could increase the immunomodulation and proliferation capacity of cells in vitro. When peritoneal macrophages were treated with various concentrations of fucoidan (1~100 μg/mL) for 24 hours, NO production was significantly increased. In addition, exposure of macrophages to 10 μg/mL of fucoidan induced a phagocytic activity. Treatment of neuroblastoma cells (SK-N-SH) with fucoidan enhanced cell proliferation and NO production in a concentration-dependent manner. These results indicate that fucoidan has both immunomodulatory and cell proliferative properties, and may thus be a a candidate for development as an immunomodulating agent.