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새로이 분리된 Klebsiella pneumoniae 균주들의 글리세롤 기반 3-hydroxypropionic acid 및 1,3-propanediol 동시 생산성 평가
고연주(Yeounjoo Ko),설은희(Eunhee Seol),순달아 세칼 바라지(Balaji Sundara Sekar),권성진(Seongjin Kwon),이재현(Jaehyeon Lee),박성훈(Sunghoon Park) 한국생물공학회 2016 KSBB Journal Vol.31 No.4
Co-production of 3-hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PDO) was suggested as an innovative strategy to overcome several limitations occurring in the single production of 3-HP from glycerol. In this study, two new isolates of Klebsiella pneumoniae, which produce less lipopolysaccharide (LPS) thus considered less pathogenic than K. pneumoniae DSM 2026, were compared and evaluated for their potential for the co-production of 3-HP and 1,3-PDO. The newly isolated strains showed significantly faster sedimentation rate than DSM, which should be beneficial for downstream processing. Analysis of genome sequences of the isolates confirmed the presence of all genes necessary for glycerol assimilation, 1,3-PDO production and biosynthesis of coenzyme B12. Co-production yield was highest under anaerobic condition while cell growth was highest under aerobic condition. Both strains showed similarly good performance for the co-production although J2B gave the slightly higher co-production yield of 0.80 mol/mol than GSC021 (0.75 mol/mol). The evaluation of the newly developed strains presented here should be useful in designing similar evaluation experiments for other microorganisms.
이산화탄소로부터 생물전기화학적 아세트산 생산을 위한 미생물 농화배양 및 군집 분석
김준형(Junhyung Kim),김영은(Young-Eun Kim),박명화(Myeonghwa Park),송영은(Young Eun Song),설은희(Eunhee Seol),김중래(Jung Rae Kim),오유관(You-Kwan Oh) 한국신재생에너지학회 2020 신재생에너지 Vol.16 No.1
Microbial electrosynthesis has recently been considered a potentially sustainable biotechnology for converting carbon dioxide (CO₂) into valuable biochemicals. In this study, bioelectrochemical acetate production from CO₂ was studied in an H-type two-chambered reactor system with an anaerobic microbial consortium. Metal-rich mud flat was used as the inoculum and incubated electrochemically for 90 days under a cathode potential of -1.1 V (vs. Ag/AgCl). Four consecutive batch cultivations resulted in a high acetate concentration and productivity of 93 m㏖/L and 7.35 m㏖/L/day, respectively. The maximal coulombic efficiency (rate of recovered acetate from supplied electrons) was estimated to be 64%. Cyclic voltammetry showed a characteristic reduction peak at -0.2~-0.4 V, implying reductive acetate generation on the cathode electrode. Furthermore, several electroactive acetate-producing microorganisms were identified based on denaturing- gradient-gel-electrophoresis (DGGE) and 16S rRNA sequence analyses. These results suggest that the mud flat can be used effectively as a microbial source for bioelectrochemical CO₂ conversion.