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      • Survey of the Fresh Rice Straw Silage in Mid-Western Costal Area in Korea

        Eui Soo Chung,Jong Geun Kim,Meing Joong Kim,Sung Seo 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

        Recently, round bale silage is very popular preservation method in Korea. In autumn, many farmers usually make silage from fresh rice straw, but the quality of rice straw silage was not good. This study was conducted to find out the silage quality of fresh rice straw in the mid-western costal area in Korea. The average DM (dry matter) content was 40.5% and pH was 5.0. Forage quality of "A" and "D" farm silage was higher than that of others area. Silage quality was also higher in "A" and "D" farm silage of west costal area. The content of lactic acid of "A" and "D" farm silage was high and acetic and butyric acid was low. The result of this experiment indicated that the quality of fresh rice straw silage made by farmers was somewhat low. So we must guide farmers to making good quality of fresh rice straw silage.

      • SCOPUSKCI등재
      • Study on the Cultivation Method of New Rice Variety, "Nokyang" for whole crop

        Jong Geun Kim,Joung Kyong Lee,Sung Seo,Eui Soo Chung,Hyung Soo Park,Won Ho Kim,Nam Chul Cho,Young Chul Lim 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

        This experiment was conducted to evaluate the effect of seeding rate on the quality and yield of whole crop rice variety, "Nokyang". Whole crop rice variety, "Nokyong", was direct seeded at 25 April. The seeding rate were four level(30, 60, 90, and 120㎏/㏊). There was not found significantly difference plant height and dry matter(DM) content among seeding rate. The average DM content was 44.1%. Dry matter yield of 30㎏/㏊ seeding rate plot was decreased compared to others treatment. The CP yield of 60㎏/㏊ seeding rate plot was the highest among treatments. Acid detergent fiber(ADF) and neutral detergent fiber(NDF) content have not been affected in relation to seeding rate. The content of TDN(total digestible nutrient) increased with increased seeding rate until 90㎏/㏊ seeding rate. The highest fresh and DM yield showed at 120㎏/㏊ seeding rate. But there was not found significantly difference among 60, 90 and 120㎏/㏊ seeding rate. Although high seeding rate increase the DM yield, 60㎏/㏊ seeding rate will be recommendable as proper seeding rate for whole crop rice.

      • KCI등재

        증례 : 알레르기 ; 에리트로마이신에 의한 아나필락시스 1예

        박재근 ( Jae Geun Park ),서의근 ( Eui Keun Seo ),조영심 ( Young Shim Cho ),장종순 ( Jong Soon Jang ),주혜진 ( Hye Jin Joo ),이승호 ( Seung Ho Lee ),김미경 ( Mi Kyeong Kim ) 대한내과학회 2010 대한내과학회지 Vol.78 No.5

        내원 21년 전 목에 있는 농양을 제거하기 위해 수술을 받았을 당시 에리트로마이신에 노출되었고, 내원 13년 전 편도염 치료를 위해 에리트로마이신 정맥투여를 하던 중 현기증과 가슴답답함, 천명과 저혈압을 경험한 환자에게 에리트로 마이신에 함유된 부용제와 보존제로 피부검사를 시행하여 피내검사에서 에리트로마이신 자체에만 강양성 반응을 나타내었으며 동시에 어지러움, 가슴답답함, 전신 소양감과 머리로 뻗치는 열감 등의 증상과 두드러기 및 천명이 나타났으며, 효소면역법으로 혈청 내 에리트로마이신 특이 IgE 항체를 확인하여 에리트로마이신 자체에 의한 제1형 과민반응인 아나필락시스임을 확인하였다. 동일 계열인 스피라마이신으로 시행한 피내 반응검사에서 양성 소견을 보여 두 항생제간의 교차항원성을 확인하였다. Macrolides antibiotics synthesized by Streptomyces strains are prescribed widely and seldom produce hypersensitivity reactions, even when administered topically. Consequently, they are considered very safe drugs. We report the case of a 32-year-old woman who developed anaphylactic shock after ingesting erythromycin, which she had taken twice before. The previous exposure to erythromycin, clinical findings, and a positive skin intradermal test with erythromycin (10 mg/mL) support the postulate that anaphylaxis was induced by erythromycin. (Korean J Med 78:660-663, 2010)

      • KCI등재

        보리 사일리지용 미생물의 발효능력 평가

        김종근(Jong Geun Kim),함준상(Jun Sang Ham),정의수(Eui Soo Chung),박형수(Hyung Soo Park),이종경(Jong Kyung Lee),정민웅(Min Woong Jung),최기춘(Ki Choon Choi),조남철(Nam Chul Jo),서성(Sung Seo) 韓國草地學會 2009 한국초지조사료학회지 Vol.29 No.3

        본 시험은 사일리지 조제시 품질 개선을 위한 젖산균 첨가제를 개발하기 위하여 2000년부터 2002년까지 국립축산과학원 초지사료과 조사료 분석실험실에서 수행하였다. 보리는 한국에서 중요한 식량작물로서 대부분이 식량으로 이용되나 곡실부분이 많아 가축의 사료로도 활용되며 특히 총체보리로 하여 반추가축의 조사료로 활용되고 있다. 본 시험은 우량 보리 사일리지에서 젖산균을 수집하여 0.02% NaN2가 함유된 MRS agar에 도말하여 생장을 보며 1차 선발한 후 MRS broth에서 다시 배양하여 생장능력과 산생성 능력을 평가하여 총 4종의 균주를 선발하였다. 선발된 균주는 그람 양성, 로드형, 카탈라제를 생성하지 않으며 생화학적 특성과 기질 이용성을 평가한 결과 Lactobacillus plantarum으로 판명되었다. 선발된 균주는 시중판매되는 첨가제 등과 함께 호숙기의 보리 사일리지에 첨가하여 2개월후 사일리지의 품질을 조사한 결과 B2-2 미생물 처리구에서 pH가 낮았고 젖산함량도 높게 나타났다. 사일리지 품질 점수와 등급에 있어서도 B2-2 처리구가 높게 나타났다. 따라서 이상의 결과를 종합하여 볼 때 B2-2 균주는 보리 사일리지용 우량 미생물로 추천되었다. This experiment was conducted to develop a new silage inoculant for barley at forage analysis laboratory, Grassland and Forages Division, National Institute of Animal Science, RDA from 2000 to 2002. Barley is very important crop in Korea. The great part of them is utilized as forage. Generally, it contains a lot of grains that are feed of animal, especially whole crop silage in ruminant. Efficient lactic acid bacteria were isolated from good barley silage by plating MRS agar containing 0.02% sodium azide, and assessed by growing and acid producing ability in MRS broth. Four lactic acid bacteria were selected, and were found to be Gram positive, rods and catalase negative and were identified to be Lactobacillus plantarum on the basis of the biochemical characteristics and utilization of substrates. Barley was ensiled at dough stage following treatment with four lactic acid bacteria, commercial inoculant, and no additive (control). After 2 months, B2-2 bacteria inoculated silage was lower pH and higher lactic acid content than others treatments. The Flieg’s score and grade of B2-2 bacteria treated silage were higher than commercial inoculant. According to this experiment, Lactobacillus plantarum B2-2 (NLRI 201) was recommendable for good silage inoculant of whole crop barley silage.

      • KCI등재

        옥수수 사일리지용 미생물의 발효능력 평가

        김종근(Jong Geun Kim),함준상(Jun Sang Ham),정의수(Eui Soo Chung),서성(Sung Seo),박형수(Hyung Soo Park) 한국초지조사료학회 2010 한국초지조사료학회지 Vol.30 No.4

        옥수수는 우리나라에서 매우 중요한 사료작물이다. 대부분 사일리지 형태로 이용이 되고 있으며 많은 비율의 곡실을 함유하고 있어 반추가축의 우수한 사료로 이용된다. 본 시험은 옥수수 사일리지용 미생물 첨가제 개발을 위해 수집된 균주의 발효능력을 평가하기 위해 수행되었다. 양질의 옥수수 사일리지에서 선발한 젖산균을 0.02%의 Sodium azide가 들어있는 MRS 배지에 접종을 한 후 균을 분리하여 MRS broth에 접종하여 산 생성 능력을 조사하였다. 총 6종의 젖산균을 선발하였고 동정결과 C3-2, B13-1 및 CC9-1은 Lactobacillus plantarum으로 판명되었고 C11-4는 Lactoba-cillus fermentum, B14-1은 Lactobacillus paracasei, 그리고 A3-1은 Leuconostoc lactis로 동정되었다. 선발된 5종의 균주와 시판되는 2종 첨가제 그리고 무처리구를 두고 황숙기에 수확된 옥수수에 접종하여 사일리지를 제조하고 2개월 후에 개봉하여 분석한 결과 B13-1 및 CC9-1 처리구의 사일리지가 pH가 낮았고 젖산함량도 높았다. 프리그 점수와 품질등급에 있어서도 B13-1 및 CC9-1 처리구에서 가장 높게 나타났다. 이상의 결과를 종합하여 볼 때 Lactobacillus plantarum B13-1 및 CC9-1는 사일리지 접종제로 사용이 권장되었다. Corn is very important forage in Korea. The great part of them is utilized as silage. Generally, it contains a lot of grains that is feed of animal. This experiment was conducted to evaluation of fermentation ability of microbes for com silage inoculant. Good lactic acid bacteria were isolated from good com silage by plating MRS agar containing 0.02% sodium azide, and assessed by growing and acid producing ability in MRS broth. Six lactic acid bacteria were selected, and were found to be Gram positive, rods and catalase negative and were identified to be lactobacillus plantarum (C3-2, B13-1, CC9-1), Lactobacillus fermentum(C11-4), Lactobacillus paracasei(B14-1), and Leuconostoc lactis (A3-1) on the basis of the biochemical characteristics and utilization of substrates. Com was ensiled at ripen stage following treatment with selected five lactic acid bacteria, two commercial inoculant, and no additive (control). After 2 month, B13-1 and CC9-1 bacteria inoculated silage were lower pH and higher lactic acid content than others treatments. The Flieg's score and grade of B13-1 and CC9-1 bacteria treated silage were higher than commercial inoculant. According to this experiment, lactobacillus plantarum B13-1 and CC9-1 strain were recommendable for good inoculant of com silage.

      • KCI등재

        총체 벼 사일리지용 미생물의 발효능력 평가

        김종근(Jong Geun Kim),함준상(Jun Sang Ham),정의수(Eui Soo Chung),윤세형(Sei Hyung Yoon),김맹중(Meing Jung Kim),박형수(Hyung Soo Park),임영철(Young Chul Lim),서성(Sung Seo) 한국초지조사료학회 2008 한국초지조사료학회지 Vol.28 No.3

        본 시험은 사료용 총체 벼 사일리지 전용 미생물 첨가제 개발을 위하여 2003년부터 2005년까지 축산연구소에서 수행되었다. 새로운 총체 벼 전용 첨가제 개발을 위해 수집된 28점의 총체 벼 사일리지로부터 미생물을 선발하여 전체 5개 균주 (R4-1, R7-1, R7-2, R10-1 및 R12-1)를 분리하였다. 분리된 균주중 4종(R4-1, R7-1, R7-2 및 R10-1)은 Lactobacillus plantarum으로 동정되었고 R12-1은 L. pentosus로 동정되었다. 황숙기에 수확된 총체 벼는 사일리지를 제조할 때 선발된 5개 균주 이외에 시중에서 판매되는 첨가제 3종을 접종을 하여 60일간 실온에서 저장한 후 평가한 결과는 다음과 같다. 저장된 사일리지의 pH와 초산 함량은 첨가제를 처리한 구에서 낮았고 젖산 함량은 높았다 (p<0.05). 특히 R7-1은 초산 함량이 가장 낮았고 젖산 함량이 가장 높았다. 조단백질 함량은 R7-2 첨가제 처리구에서 가장 높았으며 ADF 및 NDF 함량은 R7-1에서 가장 낮았다 (p<0.05). 선발된 균주들은 대부분 사일리지 품질개선효과를 나타내었으나 R7-1이 그 중에서 가장 효과적이었다. 본 미생물은 한국농용미생물 협회에 NLRI 401로 등록되었다. This experiment was conducted to study on the evaluation of fermentation ability of microbes for whole crop rice silage Inoculant at National Institute of Animal Science, RDA from 2004 to 2005. We collected 28 strains of microbes from whole crop rice silage. According to acidity and growth ability, 5 strains of microbes was isolated (R4-1, R7-1, R7-2, R10-1, R12-1). The cultures of 4 strains were identified to be Lactobacillus plantarum (R4-1, R7-1, R7-2 and R10-1) and one was identified to be Lactobacillus pentosus (R12-1). Whole crop rice was harvested at the yellow ripen stage. It was ensiled in experimental silos (20 ℓ capacity) with or without microbial additives (R4-1, R7-1, R7-2, R10-1, R12-1 and three commercial inoculant) and stored at room temperature for 60d. The pH value and acetic acid content of additivetreated silages were lower and lactic acid content was higher than those of the control (p<0.05). There was a trend for acetic acid content to be lowest and lactic acid to be highest in R7-1 treated silage. Crude protein (CP) contents of R7-2 treated silage was higher and acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents of R7-1 treated silage was lower (p<0.05). Although some strains of inoculant could improve silage quality, L. plantarum R7-1 was more effective as an inoculant for whole crop rice silage. This microbe was named NLRI 401 and registered in the Korea Agricultural Culture Collection.

      • KCI등재후보
      • KCI등재

        유산균제 첨가가 라운드베일 목초 사일리지의 품질에 미치는 영향

        김종근(Jong Geun Kim),정의수(Eui Soo Chung),서성(Sung Seo),함준상(Jun Sang Ham),윤세형(Sei Hyung Yoon),임영철(Young Chul Lim) 한국초지조사료학회 2006 한국초지조사료학회지 Vol.26 No.3

        This experiment was conducted to determine the ef fect of microbial inoculant on the quality of round baled grass silage at ex perimental field of Grassland and Forage Crops Division, National Livestock Research Institute, RDA, Suwon from 1997 to 1998. The experiment was consist of randomized block design with three replications. The treatments were three different inoculant (control, inoculant A, B and C). The contents of ether extract (EE), crude protein (CP) and in vitro dry matter digestibility (IDMD) in inoculant treatment plots were higher than those in Control. Silages treated by Inoculant A, B and C had significantly lower acidity than that of control silage (p〈0.05). Dry matter (DM) content of control silage was higher than those of inoculant treated silage. There were significant differences in organic acid contents among treatments (p〈0.05). Lactic acid was increased with inoculant treatment, but, acetic and butyric acid was decreased. The DM loss of all silages were decreased with inoculant treatment and quality grade of inoculant treated silage was higher than that of control. Results of this study indicate that addition of microbial inoculant will improve the fermentation and quality of round baled grass silage.

      • KCI등재

        비닐색이 라운드베일 목초 사일리지의 품질에 미치는 영향

        김종근(Jong Geun Kim),정의수(Eui Soo Chung),서성(Sung Seo),김맹중(Meng Jung Kim),이종경(Joung Kyong Lee),김종덕(Jong Duk Kim) 한국초지조사료학회 2006 한국초지조사료학회지 Vol.26 No.3

        This experiment was conducted to determine the effect of wrap color on the quality of round baled grass silage at experimental field of Grassland and Forage Crops Division, National Livestock Research Institute, RDA, Suwon from 1997 to 1998. The experiment was consist of randomized block design with three replications. The treatments were three wrap color(white, black and light green). Wrap color did not affect chemical composition. Fiber components(ADF and NDF) of all silages after 2 months were higher than those of forages at ensiling. Among tested wrap colors, white color wrap resulted in lower pH than others (P〈0.05) but, there was no significant difference between black and green color. Dry matter content of green color was the highest among warp colors, but there was no signifiant difference (P〈0.05). Acetic and butyric acid contents of all plots were not found significant difference among wrap color and wrap color did not influence lactic acid and organic acid concentration. The effect of wrap color on the quality grade and DM loss also were not found significant difference. Results of this study indicate that wrap color does not influence the quality of silage.

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