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- 저자 : Erfeng Kou (검색결과 1 건)
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Lihua Zhong,Xinmin Huang,Yunna Zhu,Erfeng Kou,Houcheng Liu,Guangwen Sun,Riyuan Chen,Shiwei Song 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.4
Ammonium (NH 4+ ) is generated during many endogenous metabolic processes in the leaves of plants, and there is increasing evidence that ammonium transporters (AMTs) play important roles in NH 4 + transmembrane transport and distribution. However, the expression of diff erent AMT genes is tissue-type specifi c and their functions diff er. Information about AMT genes and their expression under diff erent environmental conditions in fl owering Chinese cabbage ( Brassica campestris L.) is currently limited. Here, we isolated and characterized an AMT gene, BcAMT1;4 , in fl owering Chinese cabbage. BcAMT1;4 was localized to the plasma membrane and complemented NH 4+ transport in NH 4+ uptake-defi cient yeast. The highest expression levels of BcAMT1;4 were detected in the fl owers and leaves of fl owering Chinese cabbage. The expression of BcAMT1;4 was induced by nitrogen defi ciency and signifi cantly inhibited by the reapplication of NH 4+ (NH 4 Cl or NH 4 NO 3 ). In contrast, when plants pre-cultured in nitrate were transferred to an NH 4+ nutrient solution, BcAMT1;4 expression was signifi cantly enhanced. BcAMT1;4 exhibited a diurnal expression pattern, with higher expression levels during the light period than during the dark period, and a peak expression at the later stage of the light period. Knowledge of AMT genes in fl owering Chinese cabbage will lay a foundation for enhancing our understanding of the functional roles of diff erent AMT members in the regulation of its growth by NH 4+ , as BcAMT1;4 seems to play an important role in leaf NH 4+ transport.
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