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Xanthophylls in Microalgae : From Biosynthesis to Biotechnological Mass Production and Application
JIN, EONSEON,POLLE, JUERGEN E.W.,LEE, HONG KUM,HYUN, SANG MIN,CHANG, MAN 한국미생물 · 생명공학회 2003 Journal of microbiology and biotechnology Vol.13 No.2
Xanthophylls are oxygenated carotenoids that serve a variety of functions in photosynthetic organisms and are essential for survival of organism. Within the last decade, major advances lave been made in the elucidation of the molecular genetics and biochemistry of the xanthophyll biosynthesis pathway. Microalgae, yeast, or other microorganisms produces of the xanthophylls that are being commercially used to their own color and antioxidant properties. Currently, only a few microalgae are being considered or already being exploited for the production of high-value xanthophylls. However, new developments in molecular biology have important implications for the commercialization of microalgae, and make the genetic manipulation of the xanthophyll content of microalgae more attractive for biotechnological purposes. Accordingly, the current review summarizes the general properties of xanthophylls in microalgae and the recent developments in the biotechnological production of xanthophylls.
Paik, Sang-Min,Jin, EonSeon,Sim, Sang Jun,Jeon, Noo Li Elsevier Applied Science 2018 Bioresource technology Vol.254 No.-
<P><B>Abstract</B></P> <P>Vibrational wave treatment has been used to increase proliferation of microalgae. When directly applied at large scale, however, turbulence can offset positive effects of vibration on microalgae proliferation. Moreover, severe hydrodynamic shear fields in the bioreactor decrease cell viability that detrimentally influence maximum yieldable biomass. In this study, vibration pretreatment (between 10–30 Hz and 0.15–0.45 G) was used to prime the cells for enhanced biomass. When exposed to 10 Hz at 0.15 G for 72 h and inoculated in baffled flasks of large shear fields (0.292 Pa for the average wall shear force (aveWSF) and 184 s<SUP>−1</SUP> for the average shear strain rate (aveSSR)), microalgae showed 27% increase in biomass as well as 39% increase in corresponding amount of heterologous protein (i.e. GFP-3HA). Our results show that stress primed microalgae with vibrations can lead to improved proliferation that results in increased biomass production at industrial scale bioprocesses.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Vibration put microalgal cells into a primed state where to endure severe stresses. </LI> <LI> Primed microalgal cells grew more in harsh shear field-cultivation. </LI> <LI> Stress-priming can contributes to biomass increase and efficient large-scale bioprocess. </LI> <LI> TRP11 was cross-activated linking stresses from mild vibration and harsh shaking. </LI> </UL> </P>
Park, Seunghye,Lee, Yew,Jin, EonSeon The Korean Society of Phycology 2013 ALGAE Vol.28 No.2
Dunaliella salina and Dunaliella bardawil are well known for carotenogenesis, the overproduction of carotenoids, under stress conditions. The effect of high light (HL) and low light (LL) on the growth, morphology, photosynthetic efficiency, and the ${\beta}$-carotene and zeaxanthin production of D. salina CCAP 19/18 and D. bardawil was investigated and compared. Both strains showed similar growth kinetics under LL growth condition, but D. salina CCAP 19/18 was faster. As the light intensity increased, D. salina CCAP 19/18 cells were elongated and D. bardawil cells became larger. Both strains showed decrease of the maximum quantum yield of PSII ($F_v/F_m$) and election transport rate (ETR) under HL growth condition and D. salina CCAP 19/18 was less liable to the light stress. Both strains had about 1.8 and 5 times difference in the $O_2$ evolution rate at LL and HL conditions, respectively. The ${\beta}$-carotene and zeaxanthin production were increased as the light intensity increased in both strains. D. bardawil was more sensitive to light intensity than D. salina CCAP 19/18. The possible application of D. salina CCAP 19/18 as a carotenogenic strain will be discussed.