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Antonio Jesu´ s Mun˜oz,Francisco Espı´nola,Encarnacio´n Ruiz 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.40 No.-
The purpose of this work was to confirm the effectiveness of the bacterium Klebsiella sp. 3S1 for theremoval of lead ions from aqueous solution in a fixed-bed column. The results provided evidence thatthe bacterial biofilm formation on the support clearly improved the lead biosorption capability of thesystem. FTIR, SEM and TEM techniques provided factual evidence of the role of the bacterial biofilm onlead biosorption and the different mechanisms involved, such as cell surface binding and cytoplasmicbioaccumulation. The dynamic behavior of the column in continuous mode was well-established through breakthroughcurves, with experimental data fitting using different mathematical models, especially the Yang model. Up to 70 mg of lead was retained per gram of microbial biofilm in each cycle without loss of biosorptioncapacity compared to previous work in batch mode. In addition, the bacteria maintained theireffectiveness when the cells were inactivated after acid desorption cycles. The column was reused fourtimes after biosorbent regeneration with an elution efficiency of 99% showing that it is appropriate forindustrial applications. The use of a Klebsiella sp. 3S1 biofilm immobilized on a porous support incommercial biofilters for the removal of heavy metals from wastewater could be a competitive option.