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- 저자 : Dreo, Tanja (검색결과 4 건)
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Zachary Zeigler(Zachary Zeigler ),Malachi Votaw(Malachi Votaw ),Connor Dreos(Connor Dreos ),Lydia Durnil(Lydia Durnil ),Jamie Terran(Jamie Terran ),Danielle Akin(Danielle Akin ),Trevor Nordin(Trevor N 사피엔시아 2018 Exercise Medicine Vol.2 No.-
Objectives: Acute exercise can result in post-exercise hypotension (PEH) lasting up to 24-h. Whether exercise performed on consecutive days would lead to an accumulating PEH effect has yet to be determined. The purpose of this study was to compare daily exercise (DE) to exercise performed on alternating days (AE) and control (CON) on PEH. Methods: Sedentary men 18-30 yr with elevated blood pressure (BP) participated in this three-arm randomized cross-over trial. The primary comparison was PEH between three groups (CON, AE, DE) over time (day 1,2,3). Both exercise groups were prescribed the same exercise intensity (70-75%HRmax), and total duration of exercise (90min) on a cycle ergometer. DE performed exercise on three consecutive days (three bouts 30min). AE performed exercise on two alternating days (2 bouts 45min). Following exercise subjects remained in the laboratory for 1-h while BP was taken every 5-min. Results: Nine overweight (BMI=29.2±4.5kg/m2), young (22.7±2.4years), moderately fit (VO2peak=35.6±7.3 ml.kg.min), male subjects with elevated BP (126.2±10.4 and 73.3±6.4 mmHg) completed the study. There was a significant systolic BP condition by day effect such that on day three systolic BP (CON 119.0±9.3, AE 118.9±15.0, DE 115.0±11.9 mmHg), and diastolic BP (CON 71.9±6.6, AE 68.4±10.3, DE 67.6±6.2 mmHg) were lowest during the post-exercise DE condition (P<0.001). Additionally, DE saw a significant reduction of resting systolic BP between day 1 and day 4 (122.8±10.2 mmHg vs 113.1±12.0 mmHg; P=0.022, respectively) and a borderline significant reduction between day 1 and day 3 (122.8±10.2 mmHg vs 114.8±10.0 mmHg; P=0.051, respectively). DE saw a borderline significant resting diastolic BP reduction between day 1 and day 3 (73.2±7.2 mmHg vs 68.6±6.5 mmHg; P=0.058, respectively) and a significant reduction between day 1 and day 4 (73.2±7.2 mmHg vs 66.4±4.3 mmHg; P=0.022, respectively). Conclusions: In conclusion, the post-exercise BP lowering effect of the prior exercise session appeared to accumulate during DE such that day 3 was the lowest of all conditions and days.
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Genome Organization in Coffee as Revealed by EST PCR-RFLP, SNPs and SSR Analysis
Mishra, Manoj Kumar,Tornincasa, Patrizia,Nardi, Barbara De,Asquini, Elisa,Dreos, Rene,Terra, Lorenzo Del,Rathinavelu, Rajkumar,Rovelli, Paola,Pallavicini, Alberto,Graziosi, Giorgio 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.1
An EST-based PCR-RFLP method was employed to gain insight into genome organization in eight allopolyploid Coffea arabica cultivars and seven diploid coffee species. The PCR-amplified products at 19 EST loci were digested with 46 different restriction enzymes and size fractioned in agarose gels. Most often, the sum of the fragments length was double or more than the PCR product. In arabica, this condition could be explained by assuming the presence of duplicated loci in paralogous chromosomes and this was supported by considerable evidence of multiple loci SSR amplification. Based on the RFLP analysis, 12 EST loci were polymorphic. The level of polymorphism was higher in different species compared to the arabica varieties. Sequencing of the amplified products revealed a SNP frequency of 0.021 among diploid species and of 0.007 among arabica varieties. We propose that the involvement of two genomes in C. arabica maintains a residual level of heterozygosity in the form of paralogous chromosomes, while the self-fertilization in this species tends to drive of homozygosity. The heterozygosity of paralogous chromosomes in arabica creates valuable polymorphism essential for species diversity and survival in various ecological niches, while self-fertility tends to preserve in homozygosity many genes of functional significance.
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Genome Organization in Coffee as Revealed by EST PCRRFLP, SNPs and SSR Analysis
Manoj Kumar Mishra,Patrizia Tornincasa,Barbara De Nardi,Elisa Asquini,René Dreos,Lorenzo Del Terra,Rajkumar Rathinavelu,Paola Rovelli,Alberto Pallavicini,Giorgio Graziosi 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.1
An EST-based PCR-RFLP method was employed to gain insight into genome organization in eight allopolyploid Coffea arabica cultivars and seven diploid coffee species. The PCR-amplified products at 19 EST loci were digested with 46 different restriction enzymes and size fractioned in agarose gels. Most often, the sum of the fragments length was double or more than the PCR product. In arabica, this condition could be explained by assuming the presence of duplicated loci in paralogous chromosomes and this was supported by considerable evidence of multiple loci SSR amplification. Based on the RFLP analysis, 12 EST loci were polymorphic. The level of polymorphism was higher in different species compared to the arabica varieties. Sequencing of the amplified products revealed a SNP frequency of 0.021 among diploid species and of 0.007 among arabica varieties. We propose that the involvement of two genomes in C. arabica maintains a residual level of heterozygosity in the form of paralogous chromosomes, while the self-fertilization in this species tends to drive of homozygosity. The heterozygosity of paralogous chromosomes in arabica creates valuable polymorphism essential for species diversity and survival in various ecological niches, while self-fertility tends to preserve in homozygosity many genes of functional significance.
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Whale, Alexandra S.,Jones, Gerwyn M.,Pavš,ič,, Jernej,Dreo, Tanja,Redshaw, Nicholas,Akyü,rek, Sema,Akgö,z, Mü,slü,m,Divieto, Carla,Sassi, Maria Paola,He, Hua-Jun,Cole, Kennet American Association for Clinical Chemistry, Inc. 2018 Clinical chemistry Vol.64 No.9
<P><B>BACKGROUND:</B></P><P>Genetic testing of tumor tissue and circulating cell-free DNA for somatic variants guides patient treatment of many cancers. Such measurements will be fundamental in the future support of precision medicine. However, there are currently no primary reference measurement procedures available for nucleic acid quantification that would support translation of tests for circulating tumor DNA into routine use.</P><P><B>METHODS:</B></P><P>We assessed the accuracy of digital PCR (dPCR) for copy number quantification of a frequently occurring single-nucleotide variant in colorectal cancer (<I>KRAS</I> c.35G>A, p.Gly12Asp, from hereon termed G12D) by evaluating potential sources of uncertainty that influence dPCR measurement.</P><P><B>RESULTS:</B></P><P>Concentration values for samples of <I>KRAS</I> G12D and wild-type plasmid templates varied by <1.2-fold when measured using 5 different assays with varying detection chemistry (hydrolysis, scorpion probes, and intercalating dyes) and <1.3-fold with 4 commercial dPCR platforms. Measurement trueness of a selected dPCR assay and platform was validated by comparison with an orthogonal method (inductively coupled plasma mass spectrometry). The candidate dPCR reference measurement procedure showed linear quantification over a wide range of copies per reaction and high repeatability and interlaboratory reproducibility (CV, 2%–8% and 5%–10%, respectively).</P><P><B>CONCLUSIONS:</B></P><P>This work validates dPCR as an SI-traceable reference measurement procedure based on enumeration and demonstrates how it can be applied for assignment of copy number concentration and fractional abundance values to DNA reference materials in an aqueous solution. High-accuracy measurements using dPCR will support the implementation and traceable standardization of molecular diagnostic procedures needed for advancements in precision medicine.</P>
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