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산란계 감염 살모넬라균 억제에 대한 감귤박 특이 발효 미생물 제제의 사료 첨가 효과
TaeYoon Kang(강태윤),SyungTae Kang(강승태),YoungHo Ihn(인용호),YangHo Lee(이양호),DonYoung Cho(조돈영),Sung-Jin Lee(이성진),WonGeun Son(손원근),MoonSoo Heo(허문수),DongKee Jeong(정동기) 한국생명과학회 2010 생명과학회지 Vol.20 No.2
안전한 먹거리 생산은 축산물의 사육에서부터 시작된다. 현재 병원성 바이러스 및 균에 의해 먹거리의 안전성이 위협을 받고 있으며, 또한 항생제의 남용으로 인하여 병원성 균이 면역력을 갖게 되고 그에 따라 슈퍼 박테리아 등이 계속 생겨나고 있는 추세이다. 이런 시점에 항생제 대체 물질 개발이 절실하게 요구되고 있다. 이에 본 연구는 안전한 축산물생산과 국민의 식품안전의 최초단계인 축산업에서 항생제를 대체할 천연항생 사료첨가제를 개발하고자 본 실험을 실시하였다. 본 연구에서는 감귤박 특이 균주, 토양미생물 균주, Coenzyme Q10을 이용하였고, 무 항생제사료에 첨가하였다. 병아리 때 일정 기간 동안 사료첨가제를 급여 후 살모넬라균(Salmonella gallinarium)을 구강을 통해 주입하였다. 그리고 일정시간 동안 혈액과 분변을 채취하고 배지를 이용 살모넬라균 검사를 실시하였으며, 결과적으로 감귤박 특이 균주에서 가장 좋은 억제 효과를 얻었다. 이 결과를 살펴볼 때 병아리에서는 이러한 첨가제들이 장내 다른 병원성 균이 선점하지 못하도록 하는 것으로 사료된다. 장기의 해부를 통해서 장기의 손상 상태를 확인한 결과 감귤박 특이 균주 첨가제인 경우 간장색의 선홍색인 반면 일반 항생제 및 무 항생제 사료는 간장의 색의 황색으로 변한 것으로 미뤄 보아서 첨가제의 효과로 살모넬라균이 억제되어 간장의 손상을 방지한다는 효과를 얻었다. 이러한 결과는 천연항생제를 이용한 사육 기반 확립은 물론 먹거리 안전성을 보장하며, 사육부산물은 식물재배의 비료로 이용하여 완전 순환농업을 위한 좋은 재료가 될 수 있을 것으로 사료된다. Nowadays many people use antibiotics to protect processed foods from many pathogenic bacteria. The abuse of antibiotics, however, can run the risk of creating resistant forms of bacterium. Our study focus is on making new substances that can not only replace antibiotics but also be friendly to the environment. In our experiments, we used fermented citrus fruit, soil microbes and coenzyme Q10 as probiotics and prebiotics. Chickens in the experimental group were fed these substances via oral route while those in the control group were not. After specific time periods, blood and feces samples were collected to test for Salmonella spp.. It is interesting that fermented citrus fruit was the most effective in suppressing this bacterium. Furthermore, dissection of the experiment group chickens shows that their livers did not change to a yellow color, in contrast to the control group. The results confirmed our proposal that the chickens fed with these materials can be protected from infection by Salmonella and other pathogens. These probiotics and prebiotics are highly practical because they are natural substances that can be easily recycled in the environment. It can also be used as an animal feed ingredient because of its safety.
한국인의 인구통계학적 특성에 따른 Gut Quotient 척도 특징
김민철(Min Cheol Kim),박하영(Ha Young Park),안정혁(Junghyuk An),김승호(Seung-Ho Kim),안수경(Su Gyeong An),최은정(Eun-Jung Choi),정준호(Juneho Jeong),신동기(Dongkee Shin),박종미(Jongmi Park),최윤영(Yoonyoung Choi),이동한(Dong Han Lee) 대한약학회 2019 약학회지 Vol.63 No.4
Abstract The objective of the present study was to analyze the gut quotient scale (GQ) that we had previously developed by assessing factors on subjective assessment of overall intestinal symptoms and to conduct a comparative analysis of GQ based on demographic information. The study was also aimed to analyze the differences in GQ based on characteristics of intestinal health. Data were gathered from the responses of 1,120 individuals (male: 565, female: 555) aged 20-69 years through a nationwide quantitative survey of the general public. The demographic data were used to test the reliability and validity of the GQ scale items. The total GQ score of Koreans collected in the present study was 79.0 points. The results confirmed that people in Korea had a more negative assessment of their intestinal status based on overall subjective feeling, more so than specific attributes. They also had more negative feelings toward “bowel movement control discomfort” than “perceived intestinal discomfort” or “bowel movement discomfort.” The analysis showed no gender-based differences in the GQ of Koreans; however, there was an age-based difference among females aged 20-39 years who had a lower GQ than other age groups. Regarding marital status, married people showed lower GQ than unmarried/single people. Although there was no significant difference in GQ according to drinking types, heavy drinkers showed greater discomfort with bowel control than light drinkers. Additionally, there were no statistically significant differences in mean GQ based on education level, income level, and region. Respondents with positive subjective feelings about their intestinal state and those with healthy bowel movements showed higher GQ. The findings of the present study could be used as reference data when establishing national health policies and as basic data for pharmaceutical companies to develop marketing strategies for content related to intestinal health.