Preparation of Egg White Liquid Hydrolysate (ELH) and Its Radical-Scavenging Activity

Noh, Dong Ouk,Suh, Hyung Joo The Korean Society of Food Science and Nutrition 2015 Preventive Nutrition and Food Science Vol.20 No.3

In the present study, an optimum protease was selected to hydrolyze the egg white liquid protein for the antioxidant peptides. Alcalase treatment yielded the highest amount of ${\alpha}$-amino groups (15.27 mg/mL), while the control (no enzymatic hydrolysis) showed the lowest amount of ${\alpha}$-amino groups (1.53 mg/mL). Alcalase also gave the highest degree of hydrolysis (DH) value (43.2%) and was more efficient for egg white liquid hydrolysis than the other enzymes. The Alcalase hydrolysate had the highest radical-scavenging activity (82.5%) at a concentration of 5.0 mg/mL. The conditions for enzymatic hydrolysis of egg white liquid with Alcalase were selected as substrate : water ratio of 2:1. Five percent Alacalse treatment did not show significant (P>0.05) increases of DH and ${\alpha}$-amino nitrogen content after 24 hhydrolysis. Thirty two hour-hydrolysis with 5% Alcalase is sufficient to make antioxidative egg white liquid hydrolysate from egg white liquid. DPPH and ABTS radical-scavenging activities were significantly (P<0.05) higher after enzymatic digestion. These results suggest that active peptides released from egg-white protein are effective radical-scavengers. Thus, this approach may be useful for the preparation of potent antioxidant products.

Influence of Bile on Cellular Integrity and β-Galactosidase Activity of Lactobacillus acidophilus

Noh, Dong-Ouk 가야대학교 2000 가야대학교 논문집 Vol.9 No.-

In the presence of oxgall, the β-galactosidase activities of all strains of Lactobacillus acidophilus was increased. Among them, strain 606 showed to have the highest enzyme activity. Meanwhile, strain 107 did not show any change in the enzyme activity. As for the bile tolerance, all tested strains with the exception of strains NCFM and 606 were tolerant to the presence of oxgall, and among them, strain 4356 was the most bile-tolerant. There was no change in cellular integrity for all strains tested. This means there was no lysis of the cells, but a change in the permeability of cellular membranes of the cells, resulting in letting more lactose into the cells and increasing the enzyme activity.

Effect of Bile on β-Galactosidase Activity of Streptococcus thermophilus

NOH, DONG-OUK 가야대학교 2001 가야대학교 논문집 Vol.10 No.-

In the presence of 0.15% oxgall. the β-galactosidase activity of Streptococcus thermophilus increased. When the cells were incubated in PMN broth, only one strain (HC11) grew almost twice as much as other 3 strains, meanwhile there was no difference among the strains when they grew in MRS broth. When they were incubated either in MRS broth or in PMN broth with oxgall, the enzyme activity increased for all strains of S. thermophilus tested. Especially, the growth in PMN broth resulted in more increase of enzyme activity than that in MRS broth for all strains tested.

Preparation of Egg White Liquid Hydrolysate (ELH) and Its Radical-Scavenging Activity

Dong Ouk Noh,Hyung Joo Suh 한국식품영양과학회 2015 Preventive Nutrition and Food Science Vol.20 No.3

In the present study, an optimum protease was selected to hydrolyze the egg white liquid protein for the antioxidant peptides. Alcalase treatment yielded the highest amount of α-amino groups (15.27 mg/mL), while the control (no enzymatic hydrolysis) showed the lowest amount of?α-amino groups (1.53 mg/mL). Alcalase also gave the highest degree of hydrolysis (DH) value (43.2%) and was more efficient for egg white liquid hydrolysis than the other enzymes. The Alcalase hydrolysate had the highest radical-scavenging activity (82.5%) at a concentration of 5.0 mg/mL. The conditions for enzymatic hydrolysis of egg white liquid with Alcalase were selected as substrate : water ratio of 2:1. Five percent Alacalse treatment did not show significant (P>0.05) increases of DH and α-amino nitrogen content after 24 hhydrolysis. Thirty two hour-hydrolysis with 5% Alcalase is sufficient to make antioxidative egg white liquid hydrolysate from egg white liquid. DPPH and ABTS radical-scavenging activities were significantly (P<0.05) higher after enzymatic digestion. These results suggest that active peptides released from egg-white protein are effective radical-scavengers. Thus, this approach may be useful for the preparation of potent antioxidant products.

Lactobacillus acidophilus에 의한 콜레스테롤의 흡착

노동욱 ( Dong Ouk Noh ) 한국유가공기술과학회 1996 Journal of Dairy Science and Biotechnology (JMSB) Vol.14 No.2

Cholesterol assimilated by Lactobacillus acidophilus ATCC 43121 was not metabolically degraded in that most of it was recovered with the cells. Cells grown in the presence of cholesterol micelles and bile salts were more resistant to Iysis by sonication than those grown in their absence, suggesting a possible alteration of cellular membranes. Cholesterol assimilation occurred during growth at pH 6.0, the amount of which was more than that by cells grown without pH control. Cholesterol assimilated by cells was recovered in the membrane fractions of cells both grown at pH 6.0 and without pH control. The effect of unsaturated fatty acids on cholesterol assimilation was not clear, since there was no significant (P> 0.05) difference in the amount taken up from micelles prepared using L- -phosphatidylcholine, dioleoyl or L- -phosphatidylcholine, distearoyl. Without Tween 80, little, if any, cell growth or cholesterol uptake was observed. In the presence of 0.05% Tween 80, cholesterol uptake increased dramatically as did growth. However, as the amount of Tween 80 increased beyond 0.05%, cholesterol uptake decreased while the amount of growth remained the same.

담즙이 불가리커스 유산균의 유당분해효소인 β-galactosidase의 활성에 미치는 영향

노동욱 가야대학교 1999 가야대학교 논문집 Vol.8 No.-

In the presence of 0.15% oxgall, the β-galactosidase activity of whole cells of Lactobacillus bulgaricus was increased. However, higher concentrations of oxgall decreased the enzyme activity. In the absence of oxgall, little or no β-galactosidase activity was exhibited by the whole cells. When the cells were lysed to obtain cell-free extracts prior to assay, there was some decrease in the enzyme activity in the presence of 0.15% oxgall. Increasing the concentrations of oxgall did not further reduce the activity. Apparently, the oxgall increases the permeability of the cells up to a certain concentration(0.15%) to allow more substrate to enter, resulting in more hydrolysis of the substrate. However, at the concentrations higher than that, the enzyme activity was inhibited by the oxgall

Stent containing CD44-targeting polymeric prodrug nanoparticles that release paclitaxel and gemcitabine in a time interval-controlled manner for synergistic human biliary cancer therapy

Yun, Dayeon,Kim, Hyun-Ouk,Son, Hye Young,Choi, Yuna,Noh, Ilkoo,Lim, Jong-Woo,Kim, Jihye,Chun, Haejin,Park, Geunseon,Lee, Dong Ki,Jang, Sung Il,Jang, Eunji,Huh, Yong-Min,Haam, Seungjoo Royal Society of Chemistry 2017 Journal of Materials Chemistry B Vol. No.

<P>The use of drug-eluting stents (DESs) is a promising strategy for non-vascular diseases, especially human biliary cancer. However, the implementation of DESs suffers from two major obstacles: the side effects of drugs and the difficulty of controlling the drug release. These problems can be overcome if the stent elutes targeting nanoparticles that release drugs at time intervals that are dictated by the mechanisms of those drugs. We designed temporally controlled polymeric multi-prodrug nanoparticles (TCMPNs) that can be eluted from stents comprising polyurethane (PU) nanofiber as a polymeric matrix and paclitaxel (PTX)-loaded, CD44-targeting, hyaluronic acid-conjugated poly(lactic-<I>co</I>-glycolic acid) and gemcitabine (GEM) (P-H-G). TCMPNs enable two different types of drugs to be released temporally; PTX is released first owing to the collapse of the structure in the endosomes, and GEM, which induces synergistic anticancer activities, is hydrolyzed from P-H-G later in response to low pH. Embedded in the PU nanofiber, the TCMPNs demonstrate low initial burst behavior and sustainable release of the prodrug <I>in vitro</I>. Furthermore, TCMPN-eluting stents (TESs) exhibit continuous synergistic efficacy as available targeted cellular uptake prodrug delivery systems in tumor-bearing mice. These results demonstrate that this technology will open up cancer therapy by combining localized delivery and functional multi-drug-loaded nanoparticles.</P>

Promotion Effects of Yeast Hydrolysates and a Mixture of Safflower Seed and Gasiogapi Extract on Longitudinal Bone, Proximal Epiphysis, and Growth Hormone in Rats

Hyun-Sun Lee,Dong-Ouk Noh,Hyung-Joo Suh 한국식품영양과학회 2011 Preventive Nutrition and Food Science Vol.16 No.2

This study examined the growth effects of yeast hydrolysate (YH) and a traditional Korean herbal mixture (HM, a mixture of safflower seed and gasiogapi extract). Three-week old male SD rats were divided into the following five groups: negative control (saline), positive control (foremilk 0.5 g/㎏/day), YH (YH 0.5 g/㎏/day), HM (HM 0.2 g/㎏/day), and YH+HM (YH 0.5 g/㎏/day and HM 0.2 g/㎏/day). Tibia bone length was 9.22 ㎜ in the normal control rats, while both the YH and YH+HM groups had significantly longer tibia bones than the control rats (9.75 ㎜ and 10.46 ㎜, respectively). The proximal epiphyses of YH, HM, and YH+HM measured 0.75, 0.70, and 0.75 ㎜, respectively, while the length in the control group was 0.50 ㎜. Plasma insulin growth factor-1 (IGF-1) level was slightly higher in the YH group (1.36 ㎎/㎖) than in the control rats (1.29 ㎎/㎖), but the difference was not significant. Plasma IGF-1 level was significantly increased in the HM (1.49 ㎎/㎖) and YH+HM (1.53 ㎎/㎖) groups compared to the control group (1.29 ㎎/㎖). Growth hormone (GH) levels in YH (17.45 ng/㎖), HM (15.49 ng/㎖), and YH+HM (16.07 ng/㎖) were significantly different compared to the control group (3.63 ng/㎖).

Promotion Effects of Yeast Hydrolysates and a Mixture of Safflower Seed and Gasiogapi Extract on Longitudinal Bone, Proximal Epiphysis, and Growth Hormone in Rats

Lee, Hyun-Sun,Noh, Dong-Ouk,Suh, Hyung-Joo The Korean Society of Food Science and Nutrition 2011 Preventive Nutrition and Food Science Vol.16 No.2

This study examined the growth effects of yeast hydrolysate (YH) and a traditional Korean herbal mixture (HM, a mixture of safflower seed and gasiogapi extract). Three-week old male SD rats were divided into the following five groups: negative control (saline), positive control (foremilk 0.5 g/kg/day), YH (YH 0.5 g/kg/day), HM (HM 0.2 g/kg/day), and YH+HM (YH 0.5 g/kg/day and HM 0.2 g/kg/day). Tibia bone length was 9.22 mm in the normal control rats, while both the YH and YH+HM groups had significantly longer tibia bones than the control rats (9.75 mm and 10.46 mm, respectively). The proximal epiphyses of YH, HM, and YH+HM measured 0.75, 0.70, and 0.75 mm, respectively, while the length in the control group was 0.50 mm. Plasma insulin growth factor-1 (IGF-1) level was slightly higher in the YH group (1.36 mg/mL) than in the control rats (1.29 mg/mL), but the difference was not significant. Plasma IGF-1 level was significantly increased in the HM (1.49 mg/mL) and YH+HM (1.53 mg/mL) groups compared to the control group (1.29 mg/mL). Growth hormone (GH) levels in YH (17.45 ng/mL), HM (15.49 ng/mL), and YH+HM (16.07 ng/mL) were significantly different compared to the control group (3.63 ng/mL).

Isolation of a Phytase-Producing Bacillus sp.KHU-10 and Its Phytase Production

Choi, Yang Mun,Noh, Dong Ouk,Cho, Sung Ho,Lee, Hyo Ku,Suh, Hyung Joo,Chung, Soo Hyun 한국미생물 · 생명공학회 1999 Journal of microbiology and biotechnology Vol.9 No.2

A bacterial strain producing high level of an extracellular phytase was isolated from cooked rice and identified as a strain of Bacillus sp. and designated as Bacillus sp. KHU-10. Optimum culture conditions were investigated for the maximum productivity of phytase by Bacillus sp. KHU-10. 1.0% Maltose and 1.0% peptone with 0.5% beef extract were the best carbon source and nitrogen source, respectively. The addition of CaC1_2 stimulated the enzyme productivity with concentration between 0.01% and 0.2%, in the medium. Although sodium phosphate increased the cell mass, the enzyme activity decreased. Calcium phytate and wheat bran containing phytate did not enhance the enzyme production. Under the optimum medium. the production of the phytase reached the highest level of 0.2 unit/㎖ after 4 days of incubation.