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RISS 인기검색어
- 검색키워드
- 저자 : Dode, M.A.N. (검색결과 6 건)
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Interaction analysis of a building frame supported on pile groups
Dode, P.A.,Chore, H.S.,Agrawal, D.K. Techno-Press 2014 Coupled systems mechanics Vol.3 No.3
The study deals with the physical modeling of a typical building frame resting on pile foundation and embedded in cohesive soil mass using complete three-dimensional finite element analysis. Two different pile groups comprising four piles ($2{\times}2$) and nine piles ($3{\times}3$) are considered. Further, three different pile diameters along with the various pile spacings are considered. The elements of the superstructure frame and those of the pile foundation are descretized using twenty-node isoparametric continuum elements. The interface between the pile and pile and soil is idealized using sixteen-node isoparametric surface elements. The current study is an improved version of finite element modeling for the soil elements compared to the one reported in the literature (Chore and Ingle 2008). The soil elements are discretized using eight-, nine- and twelve-node continuum elements. Both the elements of superstructure and substructure (i.e., foundation) including soil are assumed to remain in the elastic state at all the time. The interaction analysis is carried out using sub-structure approach in the parametric study. The total stress analysis is carried out considering the immediate behaviour of the soil. The effect of various parameters of the pile foundation such as spacing in a group and number piles in a group, along with pile diameter, is evaluated on the response of superstructure. The response includes the displacement at the top of the frame and bending moment in columns. The soil-structure interaction effect is found to increase displacement in the range of 58 -152% and increase the absolute maximum positive and negative moments in the column in the range of 14-15% and 26-28%, respectively. The effect of the soil- structure interaction is observed to be significant for the configuration of the pile groups and the soil considered in the present study.
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Interaction analysis of a building frame suppoted on pile groups
Dode, P.A.,Chore, H.S.,Agrawal, D.K. Techno-Press 2014 Interaction and multiscale mechanics Vol.7 No.1
The study deals with the physical modeling of a typical building frame resting on pile foundation and embedded in cohesive soil mass using complete three-dimensional finite element analysis. Two different pile groups comprising four piles ($2{\times}2$) and nine piles ($3{\times}3$) are considered. Further, three different pile diameters along with the various pile spacings are considered. The elements of the superstructure frame and those of the pile foundation are descretized using twenty-node isoparametric continuum elements. The interface between the pile and pile cap is idealized using sixteen-node isoparametric surface elements. The current study is an improved version of finite element modeling for the soil elements compared to the one reported in the literature (Chore and Ingle 2008). The soil elements are discretized using eight-, nine- and twelve-node continuum elements. Both the elements of superstructure and substructure (i.e., foundation) including soil are assumed to remain in the elastic state at all the time. The interaction analysis is carried out using sub-structure approach in the parametric study. The total stress analysis is carried out considering the immediate behaviour of the soil. The effect of various parameters of the pile foundation such as spacing in a group and number piles in a group, along with pile diameter, is evaluated on the response of superstructure. The response includes the displacement at the top of the frame and bending moment in columns. The soil-structure interaction effect is found to increase displacement in the range of 58-152% and increase the absolute maximum positive and negative moments in the column in the range of 14-15% and 26-28%, respectively. The effect of the soil- structure interaction is observed to be significant for the configuration of the pile groups and the soil considered in the present study.
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Spricigo, J.F.W.,Morais, K.S.,Yang, B.S.,Dode, M.A.N. Academic Press 2012 Cryobiology Vol.65 No.3
The present study aimed to evaluate the effect of methyl-β-cyclodextrin (MβCD) as a cholesterol loader to change oocyte plasma membrane and increase its tolerance toward cryopreservation. The first and second experiments were conducted to investigate if MβCD could improve nuclear and cytoplasmic maturation after oocyte exposure to cold stress for 10 or 30min, respectively. No differences (P>0.05) in either experiment in the metaphase II (MII) rate of oocytes exposed to MβCD and cold stress; but these oocytes presented lower maturation rates than control groups. In the second experiment, a lower percentage of oocytes showed degenerated chromatin (P<0.05) after exposure to 2mg/mL of MβCD compared to the group exposed to 0mg/mL. However, no differences among treatments were observed in cytoplasmic maturation. Groups exposed to cold stress demonstrated a lower (P<0.05) capacity for embryonic development compared to the control groups. In the third experiment immature oocytes were exposed to MβCD and then, vitrified (cryotop). After warming, we observed that the ability to reach MII and chromatin degeneration were altered (P<0.05) by MβCD. The blastocysts rate (P<0.05) on D7 was higher in the 2mg/mLMβCD group, but an identical finding was not observed on D8 (P>0.05). Chromatin degeneration was higher in the vitrification groups. We conclude that MβCD improved nuclear maturation by reducing oocyte degeneration after cold stress or vitrification; however, more studies are required to clarify the usefulness of MβCD use in oocyte cryopreservation.
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( Bruno P. Carvalho ),( Andrielle T. M. Cunha ),( Bianca D. M. Silva ),( Regivaldo V. Sousa ),( Ligiane O. Leme ),( Margot A. N. Dode ),( Eduardo O. Melo ) 한국축산학회(구 한국동물자원과학회) 2019 한국축산학회지 Vol.61 No.2
The hG-CSF (human Granulocyte Colony-Stimulating Factor) is a growth and stimulation factor capable of inducing the proliferation of bone marrow cells, several types of leukocytes, among other hematopoietic tissue cells. hG-CSF is used in used to treat anomalies that reder a small number of circulating white blood cells, which may compromise the immune defenses of the affected person. For these reasons, the production of hG-CSF in a bioreactor system using the mammary gland of genetic modified animals is a possibility of adding value to the bovine genetic material and reducing the costs of hG-CSF production in pharmaceutical industry. In this study, we aimed the production of transgenic hG-CSF bovine through the lipofection of bovine primary fibroblasts with an hG-CSF expression cassette and cloning these fibroblasts by the somatic cell nuclear transfer (SCNT) technique. The bovine fibroblasts transfected with the hG-CSF cassette presented a stable insertion of this construct into their genome and were efficiently synchronized to G0/G1 cell cycle stage. The transgenic fibroblasts were cloned by SCNT and produced 103 transferred embryos and 2 pregnancies, one of which reached 7 months of gestation.
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