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Oxidative Stress in the Heart of Rats Infected with Trypanosoma evansi
Matheus D. Baldissera,Carine de F. Souza,Claudia M. Bertoncheli,Karine L. da Silveira,Thirssa H. Grando,Bianca C. Z. Porto,Daniela B. R. Leal,Aleksandro S. Da Silva,Ricardo E. Mendes,Lenita M. Stefani 대한기생충학열대의학회 2016 The Korean Journal of Parasitology Vol.54 No.3
( Bruno P. Carvalho ),( Andrielle T. M. Cunha ),( Bianca D. M. Silva ),( Regivaldo V. Sousa ),( Ligiane O. Leme ),( Margot A. N. Dode ),( Eduardo O. Melo ) 한국축산학회(구 한국동물자원과학회) 2019 한국축산학회지 Vol.61 No.2
The hG-CSF (human Granulocyte Colony-Stimulating Factor) is a growth and stimulation factor capable of inducing the proliferation of bone marrow cells, several types of leukocytes, among other hematopoietic tissue cells. hG-CSF is used in used to treat anomalies that reder a small number of circulating white blood cells, which may compromise the immune defenses of the affected person. For these reasons, the production of hG-CSF in a bioreactor system using the mammary gland of genetic modified animals is a possibility of adding value to the bovine genetic material and reducing the costs of hG-CSF production in pharmaceutical industry. In this study, we aimed the production of transgenic hG-CSF bovine through the lipofection of bovine primary fibroblasts with an hG-CSF expression cassette and cloning these fibroblasts by the somatic cell nuclear transfer (SCNT) technique. The bovine fibroblasts transfected with the hG-CSF cassette presented a stable insertion of this construct into their genome and were efficiently synchronized to G0/G1 cell cycle stage. The transgenic fibroblasts were cloned by SCNT and produced 103 transferred embryos and 2 pregnancies, one of which reached 7 months of gestation.